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Automated quantification of Ki-67 index associates with pathologic grade of pulmonary neuroendocrine tumors
BACKGROUND: Classification of the pulmonary neuroendocrine tumor (pNET) categories is a step-wise process identified by the presence of necrosis and number of mitoses per 2 mm(2). In neuroendocrine tumor pathology, Ki-67 was first described as a prognostic factor in the pancreas and incorporated int...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wolters Kluwer Health
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6416093/ https://www.ncbi.nlm.nih.gov/pubmed/30807354 http://dx.doi.org/10.1097/CM9.0000000000000109 |
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author | Wang, Hai-Yue Li, Zhong-Wu Sun, Wei Yang, Xin Zhou, Li-Xin Huang, Xiao-Zheng Jia, Ling Lin, Dong-Mei |
author_facet | Wang, Hai-Yue Li, Zhong-Wu Sun, Wei Yang, Xin Zhou, Li-Xin Huang, Xiao-Zheng Jia, Ling Lin, Dong-Mei |
author_sort | Wang, Hai-Yue |
collection | PubMed |
description | BACKGROUND: Classification of the pulmonary neuroendocrine tumor (pNET) categories is a step-wise process identified by the presence of necrosis and number of mitoses per 2 mm(2). In neuroendocrine tumor pathology, Ki-67 was first described as a prognostic factor in the pancreas and incorporated into the grading system of digestive tract neuroendocrine neoplasms in the 2010 WHO classification. However, the significance of Ki-67 in pNETs was still a controversial issue. This study was to investigate the potentially diagnostic value of Ki-67 in pNETs. METHODS: We retrieved 159 surgical specimens of pNETs, including 35 typical carcinoids (TCs), 2 atypical carcinoid (ACs), 28 large-cell neuroendocrine carcinomas (LCNECs), 94 small-cell lung cancers (SCLCs). Manual conventional method (MCM) and computer-assisted image analysis method (CIAM) were used to calculate the Ki-67 proliferative index. In CIAM, 6 equivalent fields (500 × 500 μm) at 10× magnification were manually annotated for digital image analysis. RESULTS: The Ki-67 index among the 4 groups with ranges of 0.38% to 12.66% for TC, 4.34% to 29.48% for AC, 30.67% to 93.74% for LCNEC, and 40.71% to 96.87% for SCLC. The cutoff value of Ki-67 index to distinguish low grade with high grade was 30.07%. For the univariate survival analyses in pNETs, both the overall survival and progression-free survival correlated with Ki-67 index. In addition, the Ki-67 index performed by CIAM was proved to be of great positive correlation with MCM. CONCLUSIONS: Ki-67 index counted by CIAM is a reliable method and can be a useful adjunct to classify the low- and high-grade NETs. |
format | Online Article Text |
id | pubmed-6416093 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Wolters Kluwer Health |
record_format | MEDLINE/PubMed |
spelling | pubmed-64160932019-03-16 Automated quantification of Ki-67 index associates with pathologic grade of pulmonary neuroendocrine tumors Wang, Hai-Yue Li, Zhong-Wu Sun, Wei Yang, Xin Zhou, Li-Xin Huang, Xiao-Zheng Jia, Ling Lin, Dong-Mei Chin Med J (Engl) Original Articles BACKGROUND: Classification of the pulmonary neuroendocrine tumor (pNET) categories is a step-wise process identified by the presence of necrosis and number of mitoses per 2 mm(2). In neuroendocrine tumor pathology, Ki-67 was first described as a prognostic factor in the pancreas and incorporated into the grading system of digestive tract neuroendocrine neoplasms in the 2010 WHO classification. However, the significance of Ki-67 in pNETs was still a controversial issue. This study was to investigate the potentially diagnostic value of Ki-67 in pNETs. METHODS: We retrieved 159 surgical specimens of pNETs, including 35 typical carcinoids (TCs), 2 atypical carcinoid (ACs), 28 large-cell neuroendocrine carcinomas (LCNECs), 94 small-cell lung cancers (SCLCs). Manual conventional method (MCM) and computer-assisted image analysis method (CIAM) were used to calculate the Ki-67 proliferative index. In CIAM, 6 equivalent fields (500 × 500 μm) at 10× magnification were manually annotated for digital image analysis. RESULTS: The Ki-67 index among the 4 groups with ranges of 0.38% to 12.66% for TC, 4.34% to 29.48% for AC, 30.67% to 93.74% for LCNEC, and 40.71% to 96.87% for SCLC. The cutoff value of Ki-67 index to distinguish low grade with high grade was 30.07%. For the univariate survival analyses in pNETs, both the overall survival and progression-free survival correlated with Ki-67 index. In addition, the Ki-67 index performed by CIAM was proved to be of great positive correlation with MCM. CONCLUSIONS: Ki-67 index counted by CIAM is a reliable method and can be a useful adjunct to classify the low- and high-grade NETs. Wolters Kluwer Health 2019-03-05 2019-02-25 /pmc/articles/PMC6416093/ /pubmed/30807354 http://dx.doi.org/10.1097/CM9.0000000000000109 Text en Copyright © 2019 The Chinese Medical Association, produced by Wolters Kluwer, Inc. under the CC-BY-NC-ND license. http://creativecommons.org/licenses/by-nc-nd/4.0 This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. http://creativecommons.org/licenses/by-nc-nd/4.0 |
spellingShingle | Original Articles Wang, Hai-Yue Li, Zhong-Wu Sun, Wei Yang, Xin Zhou, Li-Xin Huang, Xiao-Zheng Jia, Ling Lin, Dong-Mei Automated quantification of Ki-67 index associates with pathologic grade of pulmonary neuroendocrine tumors |
title | Automated quantification of Ki-67 index associates with pathologic grade of pulmonary neuroendocrine tumors |
title_full | Automated quantification of Ki-67 index associates with pathologic grade of pulmonary neuroendocrine tumors |
title_fullStr | Automated quantification of Ki-67 index associates with pathologic grade of pulmonary neuroendocrine tumors |
title_full_unstemmed | Automated quantification of Ki-67 index associates with pathologic grade of pulmonary neuroendocrine tumors |
title_short | Automated quantification of Ki-67 index associates with pathologic grade of pulmonary neuroendocrine tumors |
title_sort | automated quantification of ki-67 index associates with pathologic grade of pulmonary neuroendocrine tumors |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6416093/ https://www.ncbi.nlm.nih.gov/pubmed/30807354 http://dx.doi.org/10.1097/CM9.0000000000000109 |
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