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GlcNAc6ST3 is a keratan sulfate sulfotransferase for the protein-tyrosine phosphatase PTPRZ in the adult brain

Keratan sulfate (KS) is a carbohydrate side chain covalently attached to extracellular proteoglycans. KS is composed of disaccharide units of 6-sulfated N-acetylglucosamine (GlcNAc) and galactose. We have previously shown that GlcNAc-6-O-sulfotransferase (GlcNAc6ST) 1 encoded by Chst2 is an enzyme n...

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Autores principales: Narentuya, Takeda-Uchimura, Yoshiko, Foyez, Tahmina, Zhang, Zui, Akama, Tomoya O., Yagi, Hirokazu, Kato, Koichi, Komatsu, Yukio, Kadomatsu, Kenji, Uchimura, Kenji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6416290/
https://www.ncbi.nlm.nih.gov/pubmed/30867513
http://dx.doi.org/10.1038/s41598-019-40901-2
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author Narentuya
Takeda-Uchimura, Yoshiko
Foyez, Tahmina
Zhang, Zui
Akama, Tomoya O.
Yagi, Hirokazu
Kato, Koichi
Komatsu, Yukio
Kadomatsu, Kenji
Uchimura, Kenji
author_facet Narentuya
Takeda-Uchimura, Yoshiko
Foyez, Tahmina
Zhang, Zui
Akama, Tomoya O.
Yagi, Hirokazu
Kato, Koichi
Komatsu, Yukio
Kadomatsu, Kenji
Uchimura, Kenji
author_sort Narentuya
collection PubMed
description Keratan sulfate (KS) is a carbohydrate side chain covalently attached to extracellular proteoglycans. KS is composed of disaccharide units of 6-sulfated N-acetylglucosamine (GlcNAc) and galactose. We have previously shown that GlcNAc-6-O-sulfotransferase (GlcNAc6ST) 1 encoded by Chst2 is an enzyme necessary for the synthesis of GlcNAc-6-sulfated KS chains that are required for neuronal plasticity in the visual cortex of the mouse brain during the critical period, but not in adulthood. Here, we show that GlcNAc-6-sulfated KS recognized by the R-10G anti-KS antibody, of which the minimum epitope structure is Galß1-4GlcNAc(6S)ß1-3Galß1-4GlcNAc(6S), distributes diffusely in neuropils and presents densely in close proximity to the perineuronal region of the perineuronal net (PNN)-positive neurons in the adult visual cortex. Surprisingly, GlcNAc6ST3, which was discovered as an intestinal GlcNAc6ST encoded by Chst5, is a major brain KS sulfotransferase expressed in oligodendrocytes in adulthood. Moreover, we identified an isoform of the protein-tyrosine phosphatase PTPRZ as a R-10G-reactive KS proteoglycan. These results indicate that GlcNAc6ST3 may play a role in synthesis of a component of PNN in the adult brain, and that the KS-modified isoform of PTPRZ encoded by Ptprz1 could be an extracellular molecule associated with PNNs.
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spelling pubmed-64162902019-03-15 GlcNAc6ST3 is a keratan sulfate sulfotransferase for the protein-tyrosine phosphatase PTPRZ in the adult brain Narentuya Takeda-Uchimura, Yoshiko Foyez, Tahmina Zhang, Zui Akama, Tomoya O. Yagi, Hirokazu Kato, Koichi Komatsu, Yukio Kadomatsu, Kenji Uchimura, Kenji Sci Rep Article Keratan sulfate (KS) is a carbohydrate side chain covalently attached to extracellular proteoglycans. KS is composed of disaccharide units of 6-sulfated N-acetylglucosamine (GlcNAc) and galactose. We have previously shown that GlcNAc-6-O-sulfotransferase (GlcNAc6ST) 1 encoded by Chst2 is an enzyme necessary for the synthesis of GlcNAc-6-sulfated KS chains that are required for neuronal plasticity in the visual cortex of the mouse brain during the critical period, but not in adulthood. Here, we show that GlcNAc-6-sulfated KS recognized by the R-10G anti-KS antibody, of which the minimum epitope structure is Galß1-4GlcNAc(6S)ß1-3Galß1-4GlcNAc(6S), distributes diffusely in neuropils and presents densely in close proximity to the perineuronal region of the perineuronal net (PNN)-positive neurons in the adult visual cortex. Surprisingly, GlcNAc6ST3, which was discovered as an intestinal GlcNAc6ST encoded by Chst5, is a major brain KS sulfotransferase expressed in oligodendrocytes in adulthood. Moreover, we identified an isoform of the protein-tyrosine phosphatase PTPRZ as a R-10G-reactive KS proteoglycan. These results indicate that GlcNAc6ST3 may play a role in synthesis of a component of PNN in the adult brain, and that the KS-modified isoform of PTPRZ encoded by Ptprz1 could be an extracellular molecule associated with PNNs. Nature Publishing Group UK 2019-03-13 /pmc/articles/PMC6416290/ /pubmed/30867513 http://dx.doi.org/10.1038/s41598-019-40901-2 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Narentuya
Takeda-Uchimura, Yoshiko
Foyez, Tahmina
Zhang, Zui
Akama, Tomoya O.
Yagi, Hirokazu
Kato, Koichi
Komatsu, Yukio
Kadomatsu, Kenji
Uchimura, Kenji
GlcNAc6ST3 is a keratan sulfate sulfotransferase for the protein-tyrosine phosphatase PTPRZ in the adult brain
title GlcNAc6ST3 is a keratan sulfate sulfotransferase for the protein-tyrosine phosphatase PTPRZ in the adult brain
title_full GlcNAc6ST3 is a keratan sulfate sulfotransferase for the protein-tyrosine phosphatase PTPRZ in the adult brain
title_fullStr GlcNAc6ST3 is a keratan sulfate sulfotransferase for the protein-tyrosine phosphatase PTPRZ in the adult brain
title_full_unstemmed GlcNAc6ST3 is a keratan sulfate sulfotransferase for the protein-tyrosine phosphatase PTPRZ in the adult brain
title_short GlcNAc6ST3 is a keratan sulfate sulfotransferase for the protein-tyrosine phosphatase PTPRZ in the adult brain
title_sort glcnac6st3 is a keratan sulfate sulfotransferase for the protein-tyrosine phosphatase ptprz in the adult brain
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6416290/
https://www.ncbi.nlm.nih.gov/pubmed/30867513
http://dx.doi.org/10.1038/s41598-019-40901-2
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