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Integrative approach detects natural hybridization of sympatric lambaris species and emergence of infertile hybrids
Despite its relevance for ecology, evolution and conservation of species, natural hybridization and hybrids biology are still poorly studied in freshwater fish. Here, we tested the hypothesis that sympatric species Astyanax paranae and A. fasciatus are able to interbreed in the natural environment a...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6416303/ https://www.ncbi.nlm.nih.gov/pubmed/30867523 http://dx.doi.org/10.1038/s41598-019-40856-4 |
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author | Pinheiro, Ana Paula Barbosa Melo, Rafael Magno Costa Teixeira, Daniel Fonseca Birindelli, José Luís Olivan Carvalho, Daniel Cardoso Rizzo, Elizete |
author_facet | Pinheiro, Ana Paula Barbosa Melo, Rafael Magno Costa Teixeira, Daniel Fonseca Birindelli, José Luís Olivan Carvalho, Daniel Cardoso Rizzo, Elizete |
author_sort | Pinheiro, Ana Paula Barbosa |
collection | PubMed |
description | Despite its relevance for ecology, evolution and conservation of species, natural hybridization and hybrids biology are still poorly studied in freshwater fish. Here, we tested the hypothesis that sympatric species Astyanax paranae and A. fasciatus are able to interbreed in the natural environment and presented evidence for the first record of hybridization between these species. We analyzed anatomical traits, gametogenesis, reproductive biology, and genetic variations of the COI and S7 genes of both species and putative hybrids. Intermediate morphometric and meristic features were observed in hybrids when compared to A. paranae and A. fasciatus. Overlap in reproductive season was showed for these species, with greater reproductive activity from August to January, but hybrids did not present any sign of gonadal maturation. Oogonia and perinucleolar follicles as well as spermatogonia and primary spermatocytes were found in hybrids, but previtellogenic and vitellogenic follicles, spermatids, and spermatozoa were absent. Moreover, several alterations in gametogenesis were detected, such as interrupted meiosis in both males and females, vacuolated and degenerated germ cells, increased interstitial tissue, and presence of immune cells. Molecular analyses supported the hypothesis of hybridization between A. paranae and A. fasciatus. Overall, our multidisciplinary approach also provides strong evidence that hybrids are infertile. |
format | Online Article Text |
id | pubmed-6416303 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-64163032019-03-15 Integrative approach detects natural hybridization of sympatric lambaris species and emergence of infertile hybrids Pinheiro, Ana Paula Barbosa Melo, Rafael Magno Costa Teixeira, Daniel Fonseca Birindelli, José Luís Olivan Carvalho, Daniel Cardoso Rizzo, Elizete Sci Rep Article Despite its relevance for ecology, evolution and conservation of species, natural hybridization and hybrids biology are still poorly studied in freshwater fish. Here, we tested the hypothesis that sympatric species Astyanax paranae and A. fasciatus are able to interbreed in the natural environment and presented evidence for the first record of hybridization between these species. We analyzed anatomical traits, gametogenesis, reproductive biology, and genetic variations of the COI and S7 genes of both species and putative hybrids. Intermediate morphometric and meristic features were observed in hybrids when compared to A. paranae and A. fasciatus. Overlap in reproductive season was showed for these species, with greater reproductive activity from August to January, but hybrids did not present any sign of gonadal maturation. Oogonia and perinucleolar follicles as well as spermatogonia and primary spermatocytes were found in hybrids, but previtellogenic and vitellogenic follicles, spermatids, and spermatozoa were absent. Moreover, several alterations in gametogenesis were detected, such as interrupted meiosis in both males and females, vacuolated and degenerated germ cells, increased interstitial tissue, and presence of immune cells. Molecular analyses supported the hypothesis of hybridization between A. paranae and A. fasciatus. Overall, our multidisciplinary approach also provides strong evidence that hybrids are infertile. Nature Publishing Group UK 2019-03-13 /pmc/articles/PMC6416303/ /pubmed/30867523 http://dx.doi.org/10.1038/s41598-019-40856-4 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Pinheiro, Ana Paula Barbosa Melo, Rafael Magno Costa Teixeira, Daniel Fonseca Birindelli, José Luís Olivan Carvalho, Daniel Cardoso Rizzo, Elizete Integrative approach detects natural hybridization of sympatric lambaris species and emergence of infertile hybrids |
title | Integrative approach detects natural hybridization of sympatric lambaris species and emergence of infertile hybrids |
title_full | Integrative approach detects natural hybridization of sympatric lambaris species and emergence of infertile hybrids |
title_fullStr | Integrative approach detects natural hybridization of sympatric lambaris species and emergence of infertile hybrids |
title_full_unstemmed | Integrative approach detects natural hybridization of sympatric lambaris species and emergence of infertile hybrids |
title_short | Integrative approach detects natural hybridization of sympatric lambaris species and emergence of infertile hybrids |
title_sort | integrative approach detects natural hybridization of sympatric lambaris species and emergence of infertile hybrids |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6416303/ https://www.ncbi.nlm.nih.gov/pubmed/30867523 http://dx.doi.org/10.1038/s41598-019-40856-4 |
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