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Quantitative proteomic changes in LPS-activated monocyte-derived dendritic cells: A SWATH-MS study

Dendritic cells are key immune cells that respond to pathogens and co-ordinate many innate and adaptive immune responses. Quantitative mass spectrometry using Sequential Window Acquisition of all THeoretical fragment-ion spectra-Mass Spectrometry (SWATH-MS) was performed here to determine the global...

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Autores principales: Arya, Swati, Wiatrek-Moumoulidis, Dagmara, Synowsky, Silvia A., Shirran, Sally L., Botting, Catherine H., Powis, Simon J., Stewart, Alan J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6416353/
https://www.ncbi.nlm.nih.gov/pubmed/30867486
http://dx.doi.org/10.1038/s41598-019-40773-6
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author Arya, Swati
Wiatrek-Moumoulidis, Dagmara
Synowsky, Silvia A.
Shirran, Sally L.
Botting, Catherine H.
Powis, Simon J.
Stewart, Alan J.
author_facet Arya, Swati
Wiatrek-Moumoulidis, Dagmara
Synowsky, Silvia A.
Shirran, Sally L.
Botting, Catherine H.
Powis, Simon J.
Stewart, Alan J.
author_sort Arya, Swati
collection PubMed
description Dendritic cells are key immune cells that respond to pathogens and co-ordinate many innate and adaptive immune responses. Quantitative mass spectrometry using Sequential Window Acquisition of all THeoretical fragment-ion spectra-Mass Spectrometry (SWATH-MS) was performed here to determine the global alterations in monocyte-derived dendritic cells (moDCs) in response to stimulation with lipopolysaccharide (LPS). A moDC library of 4,666 proteins was generated and proteins were quantified at 0, 6 and 24 h post-LPS stimulation using SWATH-MS. At 6 h and 24 h post-LPS exposure, the relative abundance of 227 and 282 proteins was statistically significantly altered (p-value ≤ 0.05), respectively. Functional annotation of proteins exhibiting significant changes in expression between the various time points led to the identification of clusters of proteins implicated in distinct cellular processes including interferon and interleukin signalling, endocytosis, the ER-phagosome pathway and antigen-presentation. In SWATH-MS major histocompatibility complex (MHC) class I proteins were highly upregulated at 24 h, whilst MHC class II proteins exhibited comparatively fewer changes over this period. This study provides new detailed insight into the global proteomic changes that occur in moDCs during antigen processing and presentation and further demonstrates the potential of SWATH-MS for the quantitative study of proteins involved in cellular processes.
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spelling pubmed-64163532019-03-15 Quantitative proteomic changes in LPS-activated monocyte-derived dendritic cells: A SWATH-MS study Arya, Swati Wiatrek-Moumoulidis, Dagmara Synowsky, Silvia A. Shirran, Sally L. Botting, Catherine H. Powis, Simon J. Stewart, Alan J. Sci Rep Article Dendritic cells are key immune cells that respond to pathogens and co-ordinate many innate and adaptive immune responses. Quantitative mass spectrometry using Sequential Window Acquisition of all THeoretical fragment-ion spectra-Mass Spectrometry (SWATH-MS) was performed here to determine the global alterations in monocyte-derived dendritic cells (moDCs) in response to stimulation with lipopolysaccharide (LPS). A moDC library of 4,666 proteins was generated and proteins were quantified at 0, 6 and 24 h post-LPS stimulation using SWATH-MS. At 6 h and 24 h post-LPS exposure, the relative abundance of 227 and 282 proteins was statistically significantly altered (p-value ≤ 0.05), respectively. Functional annotation of proteins exhibiting significant changes in expression between the various time points led to the identification of clusters of proteins implicated in distinct cellular processes including interferon and interleukin signalling, endocytosis, the ER-phagosome pathway and antigen-presentation. In SWATH-MS major histocompatibility complex (MHC) class I proteins were highly upregulated at 24 h, whilst MHC class II proteins exhibited comparatively fewer changes over this period. This study provides new detailed insight into the global proteomic changes that occur in moDCs during antigen processing and presentation and further demonstrates the potential of SWATH-MS for the quantitative study of proteins involved in cellular processes. Nature Publishing Group UK 2019-03-13 /pmc/articles/PMC6416353/ /pubmed/30867486 http://dx.doi.org/10.1038/s41598-019-40773-6 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Arya, Swati
Wiatrek-Moumoulidis, Dagmara
Synowsky, Silvia A.
Shirran, Sally L.
Botting, Catherine H.
Powis, Simon J.
Stewart, Alan J.
Quantitative proteomic changes in LPS-activated monocyte-derived dendritic cells: A SWATH-MS study
title Quantitative proteomic changes in LPS-activated monocyte-derived dendritic cells: A SWATH-MS study
title_full Quantitative proteomic changes in LPS-activated monocyte-derived dendritic cells: A SWATH-MS study
title_fullStr Quantitative proteomic changes in LPS-activated monocyte-derived dendritic cells: A SWATH-MS study
title_full_unstemmed Quantitative proteomic changes in LPS-activated monocyte-derived dendritic cells: A SWATH-MS study
title_short Quantitative proteomic changes in LPS-activated monocyte-derived dendritic cells: A SWATH-MS study
title_sort quantitative proteomic changes in lps-activated monocyte-derived dendritic cells: a swath-ms study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6416353/
https://www.ncbi.nlm.nih.gov/pubmed/30867486
http://dx.doi.org/10.1038/s41598-019-40773-6
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