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HIV-specific T-cell Responses and Generalized Activation in HIV-1 Infected Long-term Non-progressors and Progressors from South India

BACKGROUND: Anti-viral cytokine expressions by cytotoxic T-cells and lower activation rates have been reported to correlate with suppressed HIV replication in long-term non-progressors (LTNP). Immune mechanisms underlying disease non-progression in LTNP might vary with HIV-1 subtype and geographical...

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Autores principales: Swathirajan, Chinnambedu Ravichandran, Vignesh, Ramachandran, Waldrop, Greer, Shanmugasundaram, Uma, Nandagopal, Pannerselvam, Solomon, Sunil Suhas, Pradeep, Amrose, Saravanan, Shanmugam, Murugavel, Kailapuri Gangatharan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bentham Science Publishers 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6416489/
https://www.ncbi.nlm.nih.gov/pubmed/30543175
http://dx.doi.org/10.2174/1570162X17666181212122607
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author Swathirajan, Chinnambedu Ravichandran
Vignesh, Ramachandran
Waldrop, Greer
Shanmugasundaram, Uma
Nandagopal, Pannerselvam
Solomon, Sunil Suhas
Pradeep, Amrose
Saravanan, Shanmugam
Murugavel, Kailapuri Gangatharan
author_facet Swathirajan, Chinnambedu Ravichandran
Vignesh, Ramachandran
Waldrop, Greer
Shanmugasundaram, Uma
Nandagopal, Pannerselvam
Solomon, Sunil Suhas
Pradeep, Amrose
Saravanan, Shanmugam
Murugavel, Kailapuri Gangatharan
author_sort Swathirajan, Chinnambedu Ravichandran
collection PubMed
description BACKGROUND: Anti-viral cytokine expressions by cytotoxic T-cells and lower activation rates have been reported to correlate with suppressed HIV replication in long-term non-progressors (LTNP). Immune mechanisms underlying disease non-progression in LTNP might vary with HIV-1 subtype and geographical locations. OBJECTIVE: This study evaluates cytokine expression and T-cells activation in relation to disease non-progression in LTNP. METHODS: HIV-1 Subtype C infected LTNP (n=20) and progressors (n=15) were enrolled and flowcytometry assays were performed to study HIV-specific CD8 T-cells expressing IL-2, IFN-γ, TNF-α and MIP-1β against gag and env peptides. CD4+ T-cell activation was evaluated by surface expression of HLADR and CD38. RESULTS: Proportions of cytokines studied did not differ significantly between LTNP and progressors, while contrasting correlations with disease progression markers were observed in LTNP. CD4+ T-cell activation rates were significantly lower in LTNP compared to progressors which indicate the potential role of T-cell activation rates in disease non-progression in LTNP. CONCLUSION: LTNP and progressors showed similar CD8+ T-cell responses, but final conclusions can be drawn only by comparing multiple immune factors in larger LTNP cohort with HIV-1 infected individuals at various levels of disease progression. A possible role of HIV-1 subtype variation and ethnic differences in addition to host-genetic and viral factors cannot be ruled out.
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spelling pubmed-64164892019-04-10 HIV-specific T-cell Responses and Generalized Activation in HIV-1 Infected Long-term Non-progressors and Progressors from South India Swathirajan, Chinnambedu Ravichandran Vignesh, Ramachandran Waldrop, Greer Shanmugasundaram, Uma Nandagopal, Pannerselvam Solomon, Sunil Suhas Pradeep, Amrose Saravanan, Shanmugam Murugavel, Kailapuri Gangatharan Curr HIV Res Article BACKGROUND: Anti-viral cytokine expressions by cytotoxic T-cells and lower activation rates have been reported to correlate with suppressed HIV replication in long-term non-progressors (LTNP). Immune mechanisms underlying disease non-progression in LTNP might vary with HIV-1 subtype and geographical locations. OBJECTIVE: This study evaluates cytokine expression and T-cells activation in relation to disease non-progression in LTNP. METHODS: HIV-1 Subtype C infected LTNP (n=20) and progressors (n=15) were enrolled and flowcytometry assays were performed to study HIV-specific CD8 T-cells expressing IL-2, IFN-γ, TNF-α and MIP-1β against gag and env peptides. CD4+ T-cell activation was evaluated by surface expression of HLADR and CD38. RESULTS: Proportions of cytokines studied did not differ significantly between LTNP and progressors, while contrasting correlations with disease progression markers were observed in LTNP. CD4+ T-cell activation rates were significantly lower in LTNP compared to progressors which indicate the potential role of T-cell activation rates in disease non-progression in LTNP. CONCLUSION: LTNP and progressors showed similar CD8+ T-cell responses, but final conclusions can be drawn only by comparing multiple immune factors in larger LTNP cohort with HIV-1 infected individuals at various levels of disease progression. A possible role of HIV-1 subtype variation and ethnic differences in addition to host-genetic and viral factors cannot be ruled out. Bentham Science Publishers 2018-07 2018-07 /pmc/articles/PMC6416489/ /pubmed/30543175 http://dx.doi.org/10.2174/1570162X17666181212122607 Text en © 2018 Bentham Science Publishers https://creativecommons.org/licenses/by-nc/4.0/legalcode This is an open access article licensed under the terms of the Creative Commons Attribution-Non-Commercial 4.0 International Public License (CC BY-NC 4.0) (https://creativecommons.org/licenses/by-nc/4.0/legalcode), which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
spellingShingle Article
Swathirajan, Chinnambedu Ravichandran
Vignesh, Ramachandran
Waldrop, Greer
Shanmugasundaram, Uma
Nandagopal, Pannerselvam
Solomon, Sunil Suhas
Pradeep, Amrose
Saravanan, Shanmugam
Murugavel, Kailapuri Gangatharan
HIV-specific T-cell Responses and Generalized Activation in HIV-1 Infected Long-term Non-progressors and Progressors from South India
title HIV-specific T-cell Responses and Generalized Activation in HIV-1 Infected Long-term Non-progressors and Progressors from South India
title_full HIV-specific T-cell Responses and Generalized Activation in HIV-1 Infected Long-term Non-progressors and Progressors from South India
title_fullStr HIV-specific T-cell Responses and Generalized Activation in HIV-1 Infected Long-term Non-progressors and Progressors from South India
title_full_unstemmed HIV-specific T-cell Responses and Generalized Activation in HIV-1 Infected Long-term Non-progressors and Progressors from South India
title_short HIV-specific T-cell Responses and Generalized Activation in HIV-1 Infected Long-term Non-progressors and Progressors from South India
title_sort hiv-specific t-cell responses and generalized activation in hiv-1 infected long-term non-progressors and progressors from south india
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6416489/
https://www.ncbi.nlm.nih.gov/pubmed/30543175
http://dx.doi.org/10.2174/1570162X17666181212122607
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