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MiR-21 promotes pterygium cell proliferation through the PTEN/AKT pathway

PURPOSE: To evaluate the effect of the overexpression of miR-21 on the properties of pterygium and examine whether miR-21 promotes the proliferation of pterygium cells through targeting the PTEN/AKT signaling pathway. METHODS: Information regarding patient gender, age, and pterygium severity was gat...

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Autores principales: Li, Xia, Dai, Yiqin, Xu, Jianjiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Vision 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6416795/
https://www.ncbi.nlm.nih.gov/pubmed/30967746
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author Li, Xia
Dai, Yiqin
Xu, Jianjiang
author_facet Li, Xia
Dai, Yiqin
Xu, Jianjiang
author_sort Li, Xia
collection PubMed
description PURPOSE: To evaluate the effect of the overexpression of miR-21 on the properties of pterygium and examine whether miR-21 promotes the proliferation of pterygium cells through targeting the PTEN/AKT signaling pathway. METHODS: Information regarding patient gender, age, and pterygium severity was gathered. Expression of miR-21 was obtained through examination of excised pterygium tissues and superior conjunctiva tissues with real-time PCR. Human pterygium fibroblasts (HPFs) were obtained from pterygium surgery and subjected to primary culture. The HPF cell lines were divided into a negative control group, an miR-21 inhibitor group, and an miR-21 inhibitor + VO-Ohpic trihydrate group, and then the cell viability and apoptosis and the expression of PTEN and AKT were examined. RESULTS: Fifty-eight subjects with unilateral primary pterygium were included. An increase in the miR-21 levels in pterygium tissue was evident compared with that in the paired normal conjunctival tissues (independent-samples t test, p<0.01). As the severity of the pterygium increased, the miR-21 levels increased (p=0.004, rs=0.373, Spearman’s rank correlation coefficient). The miR-21 inhibitor suppressed the proliferation and induced apoptosis of HPF cells through increasing the PTEN expression, and further decreasing the expression of p-AKT, which could be reversed by the PTEN inhibitor VO-Ohpic trihydrate. CONCLUSIONS: Aberrant miR-21 overexpression in the pterygium could target PTEN, which contributes to abnormal proliferation of the HPF cells through depressing the PTEN/AKT pathway. The results also suggested the potential of miR-21 and the PTEN/AKT pathway as a novel therapeutic strategy for pterygium.
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spelling pubmed-64167952019-04-09 MiR-21 promotes pterygium cell proliferation through the PTEN/AKT pathway Li, Xia Dai, Yiqin Xu, Jianjiang Mol Vis Research Article PURPOSE: To evaluate the effect of the overexpression of miR-21 on the properties of pterygium and examine whether miR-21 promotes the proliferation of pterygium cells through targeting the PTEN/AKT signaling pathway. METHODS: Information regarding patient gender, age, and pterygium severity was gathered. Expression of miR-21 was obtained through examination of excised pterygium tissues and superior conjunctiva tissues with real-time PCR. Human pterygium fibroblasts (HPFs) were obtained from pterygium surgery and subjected to primary culture. The HPF cell lines were divided into a negative control group, an miR-21 inhibitor group, and an miR-21 inhibitor + VO-Ohpic trihydrate group, and then the cell viability and apoptosis and the expression of PTEN and AKT were examined. RESULTS: Fifty-eight subjects with unilateral primary pterygium were included. An increase in the miR-21 levels in pterygium tissue was evident compared with that in the paired normal conjunctival tissues (independent-samples t test, p<0.01). As the severity of the pterygium increased, the miR-21 levels increased (p=0.004, rs=0.373, Spearman’s rank correlation coefficient). The miR-21 inhibitor suppressed the proliferation and induced apoptosis of HPF cells through increasing the PTEN expression, and further decreasing the expression of p-AKT, which could be reversed by the PTEN inhibitor VO-Ohpic trihydrate. CONCLUSIONS: Aberrant miR-21 overexpression in the pterygium could target PTEN, which contributes to abnormal proliferation of the HPF cells through depressing the PTEN/AKT pathway. The results also suggested the potential of miR-21 and the PTEN/AKT pathway as a novel therapeutic strategy for pterygium. Molecular Vision 2018-07-23 /pmc/articles/PMC6416795/ /pubmed/30967746 Text en Copyright © 2018 Molecular Vision. http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited, used for non-commercial purposes, and is not altered or transformed.
spellingShingle Research Article
Li, Xia
Dai, Yiqin
Xu, Jianjiang
MiR-21 promotes pterygium cell proliferation through the PTEN/AKT pathway
title MiR-21 promotes pterygium cell proliferation through the PTEN/AKT pathway
title_full MiR-21 promotes pterygium cell proliferation through the PTEN/AKT pathway
title_fullStr MiR-21 promotes pterygium cell proliferation through the PTEN/AKT pathway
title_full_unstemmed MiR-21 promotes pterygium cell proliferation through the PTEN/AKT pathway
title_short MiR-21 promotes pterygium cell proliferation through the PTEN/AKT pathway
title_sort mir-21 promotes pterygium cell proliferation through the pten/akt pathway
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6416795/
https://www.ncbi.nlm.nih.gov/pubmed/30967746
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AT xujianjiang mir21promotespterygiumcellproliferationthroughtheptenaktpathway