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MiR-21 promotes pterygium cell proliferation through the PTEN/AKT pathway
PURPOSE: To evaluate the effect of the overexpression of miR-21 on the properties of pterygium and examine whether miR-21 promotes the proliferation of pterygium cells through targeting the PTEN/AKT signaling pathway. METHODS: Information regarding patient gender, age, and pterygium severity was gat...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Vision
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6416795/ https://www.ncbi.nlm.nih.gov/pubmed/30967746 |
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author | Li, Xia Dai, Yiqin Xu, Jianjiang |
author_facet | Li, Xia Dai, Yiqin Xu, Jianjiang |
author_sort | Li, Xia |
collection | PubMed |
description | PURPOSE: To evaluate the effect of the overexpression of miR-21 on the properties of pterygium and examine whether miR-21 promotes the proliferation of pterygium cells through targeting the PTEN/AKT signaling pathway. METHODS: Information regarding patient gender, age, and pterygium severity was gathered. Expression of miR-21 was obtained through examination of excised pterygium tissues and superior conjunctiva tissues with real-time PCR. Human pterygium fibroblasts (HPFs) were obtained from pterygium surgery and subjected to primary culture. The HPF cell lines were divided into a negative control group, an miR-21 inhibitor group, and an miR-21 inhibitor + VO-Ohpic trihydrate group, and then the cell viability and apoptosis and the expression of PTEN and AKT were examined. RESULTS: Fifty-eight subjects with unilateral primary pterygium were included. An increase in the miR-21 levels in pterygium tissue was evident compared with that in the paired normal conjunctival tissues (independent-samples t test, p<0.01). As the severity of the pterygium increased, the miR-21 levels increased (p=0.004, rs=0.373, Spearman’s rank correlation coefficient). The miR-21 inhibitor suppressed the proliferation and induced apoptosis of HPF cells through increasing the PTEN expression, and further decreasing the expression of p-AKT, which could be reversed by the PTEN inhibitor VO-Ohpic trihydrate. CONCLUSIONS: Aberrant miR-21 overexpression in the pterygium could target PTEN, which contributes to abnormal proliferation of the HPF cells through depressing the PTEN/AKT pathway. The results also suggested the potential of miR-21 and the PTEN/AKT pathway as a novel therapeutic strategy for pterygium. |
format | Online Article Text |
id | pubmed-6416795 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Molecular Vision |
record_format | MEDLINE/PubMed |
spelling | pubmed-64167952019-04-09 MiR-21 promotes pterygium cell proliferation through the PTEN/AKT pathway Li, Xia Dai, Yiqin Xu, Jianjiang Mol Vis Research Article PURPOSE: To evaluate the effect of the overexpression of miR-21 on the properties of pterygium and examine whether miR-21 promotes the proliferation of pterygium cells through targeting the PTEN/AKT signaling pathway. METHODS: Information regarding patient gender, age, and pterygium severity was gathered. Expression of miR-21 was obtained through examination of excised pterygium tissues and superior conjunctiva tissues with real-time PCR. Human pterygium fibroblasts (HPFs) were obtained from pterygium surgery and subjected to primary culture. The HPF cell lines were divided into a negative control group, an miR-21 inhibitor group, and an miR-21 inhibitor + VO-Ohpic trihydrate group, and then the cell viability and apoptosis and the expression of PTEN and AKT were examined. RESULTS: Fifty-eight subjects with unilateral primary pterygium were included. An increase in the miR-21 levels in pterygium tissue was evident compared with that in the paired normal conjunctival tissues (independent-samples t test, p<0.01). As the severity of the pterygium increased, the miR-21 levels increased (p=0.004, rs=0.373, Spearman’s rank correlation coefficient). The miR-21 inhibitor suppressed the proliferation and induced apoptosis of HPF cells through increasing the PTEN expression, and further decreasing the expression of p-AKT, which could be reversed by the PTEN inhibitor VO-Ohpic trihydrate. CONCLUSIONS: Aberrant miR-21 overexpression in the pterygium could target PTEN, which contributes to abnormal proliferation of the HPF cells through depressing the PTEN/AKT pathway. The results also suggested the potential of miR-21 and the PTEN/AKT pathway as a novel therapeutic strategy for pterygium. Molecular Vision 2018-07-23 /pmc/articles/PMC6416795/ /pubmed/30967746 Text en Copyright © 2018 Molecular Vision. http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited, used for non-commercial purposes, and is not altered or transformed. |
spellingShingle | Research Article Li, Xia Dai, Yiqin Xu, Jianjiang MiR-21 promotes pterygium cell proliferation through the PTEN/AKT pathway |
title | MiR-21 promotes pterygium cell proliferation through the PTEN/AKT pathway |
title_full | MiR-21 promotes pterygium cell proliferation through the PTEN/AKT pathway |
title_fullStr | MiR-21 promotes pterygium cell proliferation through the PTEN/AKT pathway |
title_full_unstemmed | MiR-21 promotes pterygium cell proliferation through the PTEN/AKT pathway |
title_short | MiR-21 promotes pterygium cell proliferation through the PTEN/AKT pathway |
title_sort | mir-21 promotes pterygium cell proliferation through the pten/akt pathway |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6416795/ https://www.ncbi.nlm.nih.gov/pubmed/30967746 |
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