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The molecular mechanisms driving physiological changes after long duration space flights revealed by quantitative analysis of human blood proteins

BACKGROUND: The conditions of space flight have a significant effect on the physiological processes in the human body, yet the molecular mechanisms driving physiological changes remain unknown. METHODS: Blood samples of 18 Russian cosmonauts who had conducted long-duration missions to the Internatio...

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Autores principales: Kashirina, Daria N., Percy, Andrew J., Pastushkova, Liudmila Kh., Borchers, Christoph H., Kireev, Kirill S., Ivanisenko, Vladimir A., Kononikhin, Alexey S., Nikolaev, Eugene N., Larina, Irina M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6416832/
https://www.ncbi.nlm.nih.gov/pubmed/30871558
http://dx.doi.org/10.1186/s12920-019-0490-y
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author Kashirina, Daria N.
Percy, Andrew J.
Pastushkova, Liudmila Kh.
Borchers, Christoph H.
Kireev, Kirill S.
Ivanisenko, Vladimir A.
Kononikhin, Alexey S.
Nikolaev, Eugene N.
Larina, Irina M.
author_facet Kashirina, Daria N.
Percy, Andrew J.
Pastushkova, Liudmila Kh.
Borchers, Christoph H.
Kireev, Kirill S.
Ivanisenko, Vladimir A.
Kononikhin, Alexey S.
Nikolaev, Eugene N.
Larina, Irina M.
author_sort Kashirina, Daria N.
collection PubMed
description BACKGROUND: The conditions of space flight have a significant effect on the physiological processes in the human body, yet the molecular mechanisms driving physiological changes remain unknown. METHODS: Blood samples of 18 Russian cosmonauts who had conducted long-duration missions to the International Space Station were collected 30 days before launch and on the first and seventh days after landing. RESULTS: A panel of 125 proteins in the blood plasma was quantitated by a well-established and highly regarded targeted mass spectrometry approach. This method involves the monitoring of multiple reactions in conjunction with stable isotope-labeled standards at the University of Victoria - Genome BC Proteomics Centre. CONCLUSIONS: Reduction of circulating plasma volume during space flight and activation of fluid retention at the final stage of the flight affect the changes in plasma protein concentrations present in the first days after landing. Using an ANOVA approach, it was revealed that only 1 protein (S100A9) reliably responded to space flight conditions. This protein plays an important role in the functioning of the endothelium and can serve as a marker for activation of inflammatory reactions. Concentrations of the proteins of complement, coagulation cascades, and acute phase reactants increase in the blood of cosmonauts as measured the first day after landing. Most of these proteins’ concentrations continue to increase by the 7th day after space flight. Similar dynamics are observed for proteases and their inhibitors. Thus, there is a shift in proteolytic blood systems, which is necessary for the restoration of muscle tissue and maintenance of oncotic homeostasis. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12920-019-0490-y) contains supplementary material, which is available to authorized users.
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spelling pubmed-64168322019-03-25 The molecular mechanisms driving physiological changes after long duration space flights revealed by quantitative analysis of human blood proteins Kashirina, Daria N. Percy, Andrew J. Pastushkova, Liudmila Kh. Borchers, Christoph H. Kireev, Kirill S. Ivanisenko, Vladimir A. Kononikhin, Alexey S. Nikolaev, Eugene N. Larina, Irina M. BMC Med Genomics Research BACKGROUND: The conditions of space flight have a significant effect on the physiological processes in the human body, yet the molecular mechanisms driving physiological changes remain unknown. METHODS: Blood samples of 18 Russian cosmonauts who had conducted long-duration missions to the International Space Station were collected 30 days before launch and on the first and seventh days after landing. RESULTS: A panel of 125 proteins in the blood plasma was quantitated by a well-established and highly regarded targeted mass spectrometry approach. This method involves the monitoring of multiple reactions in conjunction with stable isotope-labeled standards at the University of Victoria - Genome BC Proteomics Centre. CONCLUSIONS: Reduction of circulating plasma volume during space flight and activation of fluid retention at the final stage of the flight affect the changes in plasma protein concentrations present in the first days after landing. Using an ANOVA approach, it was revealed that only 1 protein (S100A9) reliably responded to space flight conditions. This protein plays an important role in the functioning of the endothelium and can serve as a marker for activation of inflammatory reactions. Concentrations of the proteins of complement, coagulation cascades, and acute phase reactants increase in the blood of cosmonauts as measured the first day after landing. Most of these proteins’ concentrations continue to increase by the 7th day after space flight. Similar dynamics are observed for proteases and their inhibitors. Thus, there is a shift in proteolytic blood systems, which is necessary for the restoration of muscle tissue and maintenance of oncotic homeostasis. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12920-019-0490-y) contains supplementary material, which is available to authorized users. BioMed Central 2019-03-13 /pmc/articles/PMC6416832/ /pubmed/30871558 http://dx.doi.org/10.1186/s12920-019-0490-y Text en © The Author(s). 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Kashirina, Daria N.
Percy, Andrew J.
Pastushkova, Liudmila Kh.
Borchers, Christoph H.
Kireev, Kirill S.
Ivanisenko, Vladimir A.
Kononikhin, Alexey S.
Nikolaev, Eugene N.
Larina, Irina M.
The molecular mechanisms driving physiological changes after long duration space flights revealed by quantitative analysis of human blood proteins
title The molecular mechanisms driving physiological changes after long duration space flights revealed by quantitative analysis of human blood proteins
title_full The molecular mechanisms driving physiological changes after long duration space flights revealed by quantitative analysis of human blood proteins
title_fullStr The molecular mechanisms driving physiological changes after long duration space flights revealed by quantitative analysis of human blood proteins
title_full_unstemmed The molecular mechanisms driving physiological changes after long duration space flights revealed by quantitative analysis of human blood proteins
title_short The molecular mechanisms driving physiological changes after long duration space flights revealed by quantitative analysis of human blood proteins
title_sort molecular mechanisms driving physiological changes after long duration space flights revealed by quantitative analysis of human blood proteins
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6416832/
https://www.ncbi.nlm.nih.gov/pubmed/30871558
http://dx.doi.org/10.1186/s12920-019-0490-y
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