A facile preparation of FePt-loaded few-layer MoS(2) nanosheets nanocomposites (F-MoS(2)-FePt NCs) and their application for colorimetric detection of H(2)O(2) in living cells

BACKGROUND: Rapid and sensitive detection of H(2)O(2) especially endogenous H(2)O(2) is of great importance for series of industries including disease diagnosis and therapy. In this work, uniform FePt nanoparticles are successfully anchored onto Few-layer molybdenum disulfide nanosheets (F-MoS(2) NSs). The powder X-ray diffraction, transmission electron microscopy, UV–Vis spectra and atomic force microscopy were employed to confirm the structure of the obtained nanocomposites (F-MoS(2)-FePt NCs). The prepared nanocomposites show efficient peroxidase-like catalytic activities verified by catalyzing the peroxidation substrate 4,4′-diamino-3,3′,5,5′-tetramethylbiphenyl (TMB) with the existence of H(2)O(2). RESULTS: The optimal conditions of the constructed colorimetric sensing platform is proved as 35 °C and pH 4.2. Under optimal catalytic conditions, the detection limit for H(2)O(2) detection reaches 2.24 μM and the linear ranger is 8 μM to 300 μM. Furthermore, the proposed colorimetric sensing platform was successfully utilized to detect the intracellular H(2)O(2) of cancer cells (MCF-7). CONCLUSIONS: These findings indicated that the F-MoS(2)-FePt-TMB-H(2)O(2) system provides a potential sensing platform for hydrogen peroxide monitoring in living cells. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12951-019-0465-3) contains supplementary material, which is available to authorized users.