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Collateral damage and CRISPR genome editing
The simplicity and the versatility of clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR-Cas) systems have enabled the genetic modification of virtually every organism and offer immense therapeutic potential for the treatment of human disease. Although these...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6417652/ https://www.ncbi.nlm.nih.gov/pubmed/30870431 http://dx.doi.org/10.1371/journal.pgen.1007994 |
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author | Thomas, Mark Burgio, Gaetan Adams, David J. Iyer, Vivek |
author_facet | Thomas, Mark Burgio, Gaetan Adams, David J. Iyer, Vivek |
author_sort | Thomas, Mark |
collection | PubMed |
description | The simplicity and the versatility of clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR-Cas) systems have enabled the genetic modification of virtually every organism and offer immense therapeutic potential for the treatment of human disease. Although these systems may function efficiently within eukaryotic cells, there remain concerns about the accuracy of Cas endonuclease effectors and their use for precise gene editing. Recently, two independent reports investigating the editing accuracy of the CRISPR-Cas9 system were published by separate groups at the Wellcome Sanger Institute; our study—Iyer and colleagues [1]—defined the landscape of off-target mutations, whereas the other by Kosicki and colleagues [2] detailed the existence of on-target, potentially deleterious deletions. Although both studies found evidence of large on-target CRISPR-induced deletions, they reached seemingly very different conclusions. |
format | Online Article Text |
id | pubmed-6417652 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-64176522019-04-01 Collateral damage and CRISPR genome editing Thomas, Mark Burgio, Gaetan Adams, David J. Iyer, Vivek PLoS Genet Viewpoints The simplicity and the versatility of clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR-Cas) systems have enabled the genetic modification of virtually every organism and offer immense therapeutic potential for the treatment of human disease. Although these systems may function efficiently within eukaryotic cells, there remain concerns about the accuracy of Cas endonuclease effectors and their use for precise gene editing. Recently, two independent reports investigating the editing accuracy of the CRISPR-Cas9 system were published by separate groups at the Wellcome Sanger Institute; our study—Iyer and colleagues [1]—defined the landscape of off-target mutations, whereas the other by Kosicki and colleagues [2] detailed the existence of on-target, potentially deleterious deletions. Although both studies found evidence of large on-target CRISPR-induced deletions, they reached seemingly very different conclusions. Public Library of Science 2019-03-14 /pmc/articles/PMC6417652/ /pubmed/30870431 http://dx.doi.org/10.1371/journal.pgen.1007994 Text en © 2019 Thomas et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Viewpoints Thomas, Mark Burgio, Gaetan Adams, David J. Iyer, Vivek Collateral damage and CRISPR genome editing |
title | Collateral damage and CRISPR genome editing |
title_full | Collateral damage and CRISPR genome editing |
title_fullStr | Collateral damage and CRISPR genome editing |
title_full_unstemmed | Collateral damage and CRISPR genome editing |
title_short | Collateral damage and CRISPR genome editing |
title_sort | collateral damage and crispr genome editing |
topic | Viewpoints |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6417652/ https://www.ncbi.nlm.nih.gov/pubmed/30870431 http://dx.doi.org/10.1371/journal.pgen.1007994 |
work_keys_str_mv | AT thomasmark collateraldamageandcrisprgenomeediting AT burgiogaetan collateraldamageandcrisprgenomeediting AT adamsdavidj collateraldamageandcrisprgenomeediting AT iyervivek collateraldamageandcrisprgenomeediting |