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Collateral damage and CRISPR genome editing

The simplicity and the versatility of clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR-Cas) systems have enabled the genetic modification of virtually every organism and offer immense therapeutic potential for the treatment of human disease. Although these...

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Detalles Bibliográficos
Autores principales: Thomas, Mark, Burgio, Gaetan, Adams, David J., Iyer, Vivek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6417652/
https://www.ncbi.nlm.nih.gov/pubmed/30870431
http://dx.doi.org/10.1371/journal.pgen.1007994
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author Thomas, Mark
Burgio, Gaetan
Adams, David J.
Iyer, Vivek
author_facet Thomas, Mark
Burgio, Gaetan
Adams, David J.
Iyer, Vivek
author_sort Thomas, Mark
collection PubMed
description The simplicity and the versatility of clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR-Cas) systems have enabled the genetic modification of virtually every organism and offer immense therapeutic potential for the treatment of human disease. Although these systems may function efficiently within eukaryotic cells, there remain concerns about the accuracy of Cas endonuclease effectors and their use for precise gene editing. Recently, two independent reports investigating the editing accuracy of the CRISPR-Cas9 system were published by separate groups at the Wellcome Sanger Institute; our study—Iyer and colleagues [1]—defined the landscape of off-target mutations, whereas the other by Kosicki and colleagues [2] detailed the existence of on-target, potentially deleterious deletions. Although both studies found evidence of large on-target CRISPR-induced deletions, they reached seemingly very different conclusions.
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spelling pubmed-64176522019-04-01 Collateral damage and CRISPR genome editing Thomas, Mark Burgio, Gaetan Adams, David J. Iyer, Vivek PLoS Genet Viewpoints The simplicity and the versatility of clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR-Cas) systems have enabled the genetic modification of virtually every organism and offer immense therapeutic potential for the treatment of human disease. Although these systems may function efficiently within eukaryotic cells, there remain concerns about the accuracy of Cas endonuclease effectors and their use for precise gene editing. Recently, two independent reports investigating the editing accuracy of the CRISPR-Cas9 system were published by separate groups at the Wellcome Sanger Institute; our study—Iyer and colleagues [1]—defined the landscape of off-target mutations, whereas the other by Kosicki and colleagues [2] detailed the existence of on-target, potentially deleterious deletions. Although both studies found evidence of large on-target CRISPR-induced deletions, they reached seemingly very different conclusions. Public Library of Science 2019-03-14 /pmc/articles/PMC6417652/ /pubmed/30870431 http://dx.doi.org/10.1371/journal.pgen.1007994 Text en © 2019 Thomas et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Viewpoints
Thomas, Mark
Burgio, Gaetan
Adams, David J.
Iyer, Vivek
Collateral damage and CRISPR genome editing
title Collateral damage and CRISPR genome editing
title_full Collateral damage and CRISPR genome editing
title_fullStr Collateral damage and CRISPR genome editing
title_full_unstemmed Collateral damage and CRISPR genome editing
title_short Collateral damage and CRISPR genome editing
title_sort collateral damage and crispr genome editing
topic Viewpoints
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6417652/
https://www.ncbi.nlm.nih.gov/pubmed/30870431
http://dx.doi.org/10.1371/journal.pgen.1007994
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