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Impaired hepatic mitochondrial function during early lactation in dairy cows: Association with protein lysine acetylation

Early lactation is an energy-demanding period for dairy cows which may lead to negative energy balance, threatening animal health and consequently productivity. Herein we studied hepatic mitochondrial function in Holstein-Friesian multiparous dairy cows during lactation, under two different feeding...

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Autores principales: García-Roche, Mercedes, Casal, Alberto, Mattiauda, Diego A., Ceriani, Mateo, Jasinsky, Alejandra, Mastrogiovanni, Mauricio, Trostchansky, Andrés, Carriquiry, Mariana, Cassina, Adriana, Quijano, Celia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6417696/
https://www.ncbi.nlm.nih.gov/pubmed/30870481
http://dx.doi.org/10.1371/journal.pone.0213780
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author García-Roche, Mercedes
Casal, Alberto
Mattiauda, Diego A.
Ceriani, Mateo
Jasinsky, Alejandra
Mastrogiovanni, Mauricio
Trostchansky, Andrés
Carriquiry, Mariana
Cassina, Adriana
Quijano, Celia
author_facet García-Roche, Mercedes
Casal, Alberto
Mattiauda, Diego A.
Ceriani, Mateo
Jasinsky, Alejandra
Mastrogiovanni, Mauricio
Trostchansky, Andrés
Carriquiry, Mariana
Cassina, Adriana
Quijano, Celia
author_sort García-Roche, Mercedes
collection PubMed
description Early lactation is an energy-demanding period for dairy cows which may lead to negative energy balance, threatening animal health and consequently productivity. Herein we studied hepatic mitochondrial function in Holstein-Friesian multiparous dairy cows during lactation, under two different feeding strategies. During the first 180 days postpartum the cows were fed a total mixed ration (70% forage: 30% concentrate) ad libitum (non-grazing group, G0) or grazed Festuca arundinacea or Mendicago sativa plus supplementation (grazing group, G1). From 180 to 250 days postpartum, all cows grazed Festuca arundinacea and were supplemented with total mixed ration. Mitochondrial function was assessed measuring oxygen consumption rate in liver biopsies and revealed that maximum respiratory rate decreased significantly in grazing cows during early lactation, yet was unchanged in non-grazing cows during the lactation curve. While no differences could be found in mitochondrial content or oxidative stress markers, a significant increase in protein lysine acetylation was found in grazing cows during early lactation but not in cows from the non-grazing group. Mitochondrial acetylation positively correlated with liver triglycerides and β-hydroxybutyrate plasma levels, well-known markers of negative energy balance, while a negative correlation was found with the maximum respiratory rate and sirtuin 3 levels. To our knowledge this is the first report of mitochondrial function in liver biopsies of dairy cows during lactation. On the whole our results indicate that mitochondrial function is impaired during early lactation in grazing cows and that acetylation may account for changes in mitochondrial function in this period. Additionally, our results suggest that feeding total mixed ration during early lactation may be an efficient protective strategy.
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spelling pubmed-64176962019-04-12 Impaired hepatic mitochondrial function during early lactation in dairy cows: Association with protein lysine acetylation García-Roche, Mercedes Casal, Alberto Mattiauda, Diego A. Ceriani, Mateo Jasinsky, Alejandra Mastrogiovanni, Mauricio Trostchansky, Andrés Carriquiry, Mariana Cassina, Adriana Quijano, Celia PLoS One Research Article Early lactation is an energy-demanding period for dairy cows which may lead to negative energy balance, threatening animal health and consequently productivity. Herein we studied hepatic mitochondrial function in Holstein-Friesian multiparous dairy cows during lactation, under two different feeding strategies. During the first 180 days postpartum the cows were fed a total mixed ration (70% forage: 30% concentrate) ad libitum (non-grazing group, G0) or grazed Festuca arundinacea or Mendicago sativa plus supplementation (grazing group, G1). From 180 to 250 days postpartum, all cows grazed Festuca arundinacea and were supplemented with total mixed ration. Mitochondrial function was assessed measuring oxygen consumption rate in liver biopsies and revealed that maximum respiratory rate decreased significantly in grazing cows during early lactation, yet was unchanged in non-grazing cows during the lactation curve. While no differences could be found in mitochondrial content or oxidative stress markers, a significant increase in protein lysine acetylation was found in grazing cows during early lactation but not in cows from the non-grazing group. Mitochondrial acetylation positively correlated with liver triglycerides and β-hydroxybutyrate plasma levels, well-known markers of negative energy balance, while a negative correlation was found with the maximum respiratory rate and sirtuin 3 levels. To our knowledge this is the first report of mitochondrial function in liver biopsies of dairy cows during lactation. On the whole our results indicate that mitochondrial function is impaired during early lactation in grazing cows and that acetylation may account for changes in mitochondrial function in this period. Additionally, our results suggest that feeding total mixed ration during early lactation may be an efficient protective strategy. Public Library of Science 2019-03-14 /pmc/articles/PMC6417696/ /pubmed/30870481 http://dx.doi.org/10.1371/journal.pone.0213780 Text en © 2019 García-Roche et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
García-Roche, Mercedes
Casal, Alberto
Mattiauda, Diego A.
Ceriani, Mateo
Jasinsky, Alejandra
Mastrogiovanni, Mauricio
Trostchansky, Andrés
Carriquiry, Mariana
Cassina, Adriana
Quijano, Celia
Impaired hepatic mitochondrial function during early lactation in dairy cows: Association with protein lysine acetylation
title Impaired hepatic mitochondrial function during early lactation in dairy cows: Association with protein lysine acetylation
title_full Impaired hepatic mitochondrial function during early lactation in dairy cows: Association with protein lysine acetylation
title_fullStr Impaired hepatic mitochondrial function during early lactation in dairy cows: Association with protein lysine acetylation
title_full_unstemmed Impaired hepatic mitochondrial function during early lactation in dairy cows: Association with protein lysine acetylation
title_short Impaired hepatic mitochondrial function during early lactation in dairy cows: Association with protein lysine acetylation
title_sort impaired hepatic mitochondrial function during early lactation in dairy cows: association with protein lysine acetylation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6417696/
https://www.ncbi.nlm.nih.gov/pubmed/30870481
http://dx.doi.org/10.1371/journal.pone.0213780
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