Cargando…

Adhesion and invasion of gingival epithelial cells by Porphyromonas gulae

Porphyromonas gulae, an animal periodontal pathogen, possess fimbriae classified into three genotypes (A-C) based on the diversity of fimA genes encoding FimA. Accumulating evidence suggests that P. gulae strains with type C fimbriae are more virulent as compared to those with other types. The abili...

Descripción completa

Detalles Bibliográficos
Autores principales: Inaba, Hiroaki, Nomura, Ryota, Kato, Yukio, Takeuchi, Hiroki, Amano, Atsuo, Asai, Fumitoshi, Nakano, Kazuhiko, Lamont, Richard J., Matsumoto-Nakano, Michiyo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6417775/
https://www.ncbi.nlm.nih.gov/pubmed/30870452
http://dx.doi.org/10.1371/journal.pone.0213309
_version_ 1783403621599150080
author Inaba, Hiroaki
Nomura, Ryota
Kato, Yukio
Takeuchi, Hiroki
Amano, Atsuo
Asai, Fumitoshi
Nakano, Kazuhiko
Lamont, Richard J.
Matsumoto-Nakano, Michiyo
author_facet Inaba, Hiroaki
Nomura, Ryota
Kato, Yukio
Takeuchi, Hiroki
Amano, Atsuo
Asai, Fumitoshi
Nakano, Kazuhiko
Lamont, Richard J.
Matsumoto-Nakano, Michiyo
author_sort Inaba, Hiroaki
collection PubMed
description Porphyromonas gulae, an animal periodontal pathogen, possess fimbriae classified into three genotypes (A-C) based on the diversity of fimA genes encoding FimA. Accumulating evidence suggests that P. gulae strains with type C fimbriae are more virulent as compared to those with other types. The ability of these organisms to adhere to and invade gingival epithelial cells has yet to be examined. P. gulae showed the greatest levels of adhesion and invasion at a multiplicity of infection of 100 for 90 min. P. gulae type C and some type B strains invaded gingival epithelial cells at significantly greater levels than the other strains, at the same level of efficiency as P. gingivalis with type II fimbriae. Adhesion and invasion of gingival epithelial cells by P. gulae were inhibited by cytochalasin D and sodium azide, indicating the requirements of actin polymerization and energy metabolism for those activities. Invasion within gingival epithelial cells was blocked by staurosporine, whereas those inhibitors showed little effects on adhesion, while nocodazole and cycloheximide had negligible effects on either adhesion or invasion. P. gulae proteases were found to be essential for adhesion and invasion of gingival epithelial cells, while its DNA and RNA, and protein synthesis were unnecessary for those activities. Additionally, α5β1 integrin antibodies significantly inhibited adhesion and invasion by P. gulae. This is the first report to characterize P. gulae adhesion and invasion of human gingival epithelial cells.
format Online
Article
Text
id pubmed-6417775
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-64177752019-04-01 Adhesion and invasion of gingival epithelial cells by Porphyromonas gulae Inaba, Hiroaki Nomura, Ryota Kato, Yukio Takeuchi, Hiroki Amano, Atsuo Asai, Fumitoshi Nakano, Kazuhiko Lamont, Richard J. Matsumoto-Nakano, Michiyo PLoS One Research Article Porphyromonas gulae, an animal periodontal pathogen, possess fimbriae classified into three genotypes (A-C) based on the diversity of fimA genes encoding FimA. Accumulating evidence suggests that P. gulae strains with type C fimbriae are more virulent as compared to those with other types. The ability of these organisms to adhere to and invade gingival epithelial cells has yet to be examined. P. gulae showed the greatest levels of adhesion and invasion at a multiplicity of infection of 100 for 90 min. P. gulae type C and some type B strains invaded gingival epithelial cells at significantly greater levels than the other strains, at the same level of efficiency as P. gingivalis with type II fimbriae. Adhesion and invasion of gingival epithelial cells by P. gulae were inhibited by cytochalasin D and sodium azide, indicating the requirements of actin polymerization and energy metabolism for those activities. Invasion within gingival epithelial cells was blocked by staurosporine, whereas those inhibitors showed little effects on adhesion, while nocodazole and cycloheximide had negligible effects on either adhesion or invasion. P. gulae proteases were found to be essential for adhesion and invasion of gingival epithelial cells, while its DNA and RNA, and protein synthesis were unnecessary for those activities. Additionally, α5β1 integrin antibodies significantly inhibited adhesion and invasion by P. gulae. This is the first report to characterize P. gulae adhesion and invasion of human gingival epithelial cells. Public Library of Science 2019-03-14 /pmc/articles/PMC6417775/ /pubmed/30870452 http://dx.doi.org/10.1371/journal.pone.0213309 Text en © 2019 Inaba et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Inaba, Hiroaki
Nomura, Ryota
Kato, Yukio
Takeuchi, Hiroki
Amano, Atsuo
Asai, Fumitoshi
Nakano, Kazuhiko
Lamont, Richard J.
Matsumoto-Nakano, Michiyo
Adhesion and invasion of gingival epithelial cells by Porphyromonas gulae
title Adhesion and invasion of gingival epithelial cells by Porphyromonas gulae
title_full Adhesion and invasion of gingival epithelial cells by Porphyromonas gulae
title_fullStr Adhesion and invasion of gingival epithelial cells by Porphyromonas gulae
title_full_unstemmed Adhesion and invasion of gingival epithelial cells by Porphyromonas gulae
title_short Adhesion and invasion of gingival epithelial cells by Porphyromonas gulae
title_sort adhesion and invasion of gingival epithelial cells by porphyromonas gulae
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6417775/
https://www.ncbi.nlm.nih.gov/pubmed/30870452
http://dx.doi.org/10.1371/journal.pone.0213309
work_keys_str_mv AT inabahiroaki adhesionandinvasionofgingivalepithelialcellsbyporphyromonasgulae
AT nomuraryota adhesionandinvasionofgingivalepithelialcellsbyporphyromonasgulae
AT katoyukio adhesionandinvasionofgingivalepithelialcellsbyporphyromonasgulae
AT takeuchihiroki adhesionandinvasionofgingivalepithelialcellsbyporphyromonasgulae
AT amanoatsuo adhesionandinvasionofgingivalepithelialcellsbyporphyromonasgulae
AT asaifumitoshi adhesionandinvasionofgingivalepithelialcellsbyporphyromonasgulae
AT nakanokazuhiko adhesionandinvasionofgingivalepithelialcellsbyporphyromonasgulae
AT lamontrichardj adhesionandinvasionofgingivalepithelialcellsbyporphyromonasgulae
AT matsumotonakanomichiyo adhesionandinvasionofgingivalepithelialcellsbyporphyromonasgulae