Cargando…
The Dietary Flavonoid, Luteolin, Negatively Affects Neuronal Differentiation
Luteolin, a polyphenolic plant flavonoid, has been attributed with numerous beneficial properties like anti-cancer, antioxidant, and anti-inflammatory action. Luteolin has been reported earlier to be neuroprotective in models of spinal cord injury and traumatic brain injury and also induces neurite...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6418693/ https://www.ncbi.nlm.nih.gov/pubmed/30906251 http://dx.doi.org/10.3389/fnmol.2019.00041 |
_version_ | 1783403789413253120 |
---|---|
author | Swaminathan, Amrutha Basu, Moumita Bekri, Abdelhamid Drapeau, Pierre Kundu, Tapas K. |
author_facet | Swaminathan, Amrutha Basu, Moumita Bekri, Abdelhamid Drapeau, Pierre Kundu, Tapas K. |
author_sort | Swaminathan, Amrutha |
collection | PubMed |
description | Luteolin, a polyphenolic plant flavonoid, has been attributed with numerous beneficial properties like anti-cancer, antioxidant, and anti-inflammatory action. Luteolin has been reported earlier to be neuroprotective in models of spinal cord injury and traumatic brain injury and also induces neurite outgrowth in PC12 cells. However, the effect of luteolin on early differentiation, which might be important for its beneficial effects, is unknown. In this report, we show that luteolin negatively affects early differentiation of embryonic stem cells, hampering the formation of embryoid bodies. At later stages of differentiation, luteolin specifically inhibits neuronal differentiation, where the expression of early neuronal markers is suppressed, whereas luteolin treatment does not inhibit expression of meso- and endodermal markers. Further, in a developing zebrafish model, luteolin treatment leads to fewer numbers of mitotic cells in the brain. These specific effects of luteolin on neuronal differentiation could possibly be due to its ability to inhibit the lysine acetyltransferase, p300, since the structurally closely related p300 non-inhibitor flavonoid, apigenin, does not inhibit neuronal differentiation. These results show that luteolin perturbs neuronal differentiation of embryonic stem cells. |
format | Online Article Text |
id | pubmed-6418693 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-64186932019-03-22 The Dietary Flavonoid, Luteolin, Negatively Affects Neuronal Differentiation Swaminathan, Amrutha Basu, Moumita Bekri, Abdelhamid Drapeau, Pierre Kundu, Tapas K. Front Mol Neurosci Neuroscience Luteolin, a polyphenolic plant flavonoid, has been attributed with numerous beneficial properties like anti-cancer, antioxidant, and anti-inflammatory action. Luteolin has been reported earlier to be neuroprotective in models of spinal cord injury and traumatic brain injury and also induces neurite outgrowth in PC12 cells. However, the effect of luteolin on early differentiation, which might be important for its beneficial effects, is unknown. In this report, we show that luteolin negatively affects early differentiation of embryonic stem cells, hampering the formation of embryoid bodies. At later stages of differentiation, luteolin specifically inhibits neuronal differentiation, where the expression of early neuronal markers is suppressed, whereas luteolin treatment does not inhibit expression of meso- and endodermal markers. Further, in a developing zebrafish model, luteolin treatment leads to fewer numbers of mitotic cells in the brain. These specific effects of luteolin on neuronal differentiation could possibly be due to its ability to inhibit the lysine acetyltransferase, p300, since the structurally closely related p300 non-inhibitor flavonoid, apigenin, does not inhibit neuronal differentiation. These results show that luteolin perturbs neuronal differentiation of embryonic stem cells. Frontiers Media S.A. 2019-03-08 /pmc/articles/PMC6418693/ /pubmed/30906251 http://dx.doi.org/10.3389/fnmol.2019.00041 Text en Copyright © 2019 Swaminathan, Basu, Bekri, Drapeau and Kundu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Neuroscience Swaminathan, Amrutha Basu, Moumita Bekri, Abdelhamid Drapeau, Pierre Kundu, Tapas K. The Dietary Flavonoid, Luteolin, Negatively Affects Neuronal Differentiation |
title | The Dietary Flavonoid, Luteolin, Negatively Affects Neuronal Differentiation |
title_full | The Dietary Flavonoid, Luteolin, Negatively Affects Neuronal Differentiation |
title_fullStr | The Dietary Flavonoid, Luteolin, Negatively Affects Neuronal Differentiation |
title_full_unstemmed | The Dietary Flavonoid, Luteolin, Negatively Affects Neuronal Differentiation |
title_short | The Dietary Flavonoid, Luteolin, Negatively Affects Neuronal Differentiation |
title_sort | dietary flavonoid, luteolin, negatively affects neuronal differentiation |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6418693/ https://www.ncbi.nlm.nih.gov/pubmed/30906251 http://dx.doi.org/10.3389/fnmol.2019.00041 |
work_keys_str_mv | AT swaminathanamrutha thedietaryflavonoidluteolinnegativelyaffectsneuronaldifferentiation AT basumoumita thedietaryflavonoidluteolinnegativelyaffectsneuronaldifferentiation AT bekriabdelhamid thedietaryflavonoidluteolinnegativelyaffectsneuronaldifferentiation AT drapeaupierre thedietaryflavonoidluteolinnegativelyaffectsneuronaldifferentiation AT kundutapask thedietaryflavonoidluteolinnegativelyaffectsneuronaldifferentiation AT swaminathanamrutha dietaryflavonoidluteolinnegativelyaffectsneuronaldifferentiation AT basumoumita dietaryflavonoidluteolinnegativelyaffectsneuronaldifferentiation AT bekriabdelhamid dietaryflavonoidluteolinnegativelyaffectsneuronaldifferentiation AT drapeaupierre dietaryflavonoidluteolinnegativelyaffectsneuronaldifferentiation AT kundutapask dietaryflavonoidluteolinnegativelyaffectsneuronaldifferentiation |