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Preparation and Characterization of Breathable Hemostatic Hydrogel Dressings and Determination of Their Effects on Full-Thickness Defects

Hydrogel-based wound dressings provide a cooling sensation, a moist environment, and act as a barrier to microbes for wounds. In this study, a series of soft, flexible, porous non-stick hydrogel dressings were prepared through the simple repeated freeze-thawing of a poly(vinyl alcohol), human-like c...

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Detalles Bibliográficos
Autores principales: Pan, Hong, Fan, Daidi, Cao, Wei, Zhu, Chenhui, Duan, Zhiguang, Fu, Rongzhan, Li, Xian, Ma, Xiaoxuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6418977/
https://www.ncbi.nlm.nih.gov/pubmed/30966027
http://dx.doi.org/10.3390/polym9120727
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author Pan, Hong
Fan, Daidi
Cao, Wei
Zhu, Chenhui
Duan, Zhiguang
Fu, Rongzhan
Li, Xian
Ma, Xiaoxuan
author_facet Pan, Hong
Fan, Daidi
Cao, Wei
Zhu, Chenhui
Duan, Zhiguang
Fu, Rongzhan
Li, Xian
Ma, Xiaoxuan
author_sort Pan, Hong
collection PubMed
description Hydrogel-based wound dressings provide a cooling sensation, a moist environment, and act as a barrier to microbes for wounds. In this study, a series of soft, flexible, porous non-stick hydrogel dressings were prepared through the simple repeated freeze-thawing of a poly(vinyl alcohol), human-like collagen (or and carboxymethyl chitosan) mixed solution rather than chemical cross-linking and Tween80 was added as pore-forming agent for cutaneous wound healing. Some of their physical and chemical properties were characterized. Interestingly, hydrogel PVA-HLC-T80 and PVA-HLC-CS-T80 presented excellent swelling ratios, bacterial barrier activity, moisture vapor permeability, hemostasis activity and biocompatibility. Furthermore, in vivo evaluation of the healing capacity of these two hydrogels was checked by creating a full-thickness wound defect (1.3 cm × 1.3 cm) in rabbit. Macroscopic observation and subsequent hematoxylin eosin staining (H&E) staining and transmission electron microscopy (TEM) analysis at regular time intervals for 18 days revealed that the hydrogels significantly enhanced wound healing by reducing inflammation, promoting granulation tissue formation, collagen deposition and accelerating re-epithelialization. Taken together, the obtained data strongly encourage the use of these multifunctional hydrogels for skin wound dressings.
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spelling pubmed-64189772019-04-02 Preparation and Characterization of Breathable Hemostatic Hydrogel Dressings and Determination of Their Effects on Full-Thickness Defects Pan, Hong Fan, Daidi Cao, Wei Zhu, Chenhui Duan, Zhiguang Fu, Rongzhan Li, Xian Ma, Xiaoxuan Polymers (Basel) Article Hydrogel-based wound dressings provide a cooling sensation, a moist environment, and act as a barrier to microbes for wounds. In this study, a series of soft, flexible, porous non-stick hydrogel dressings were prepared through the simple repeated freeze-thawing of a poly(vinyl alcohol), human-like collagen (or and carboxymethyl chitosan) mixed solution rather than chemical cross-linking and Tween80 was added as pore-forming agent for cutaneous wound healing. Some of their physical and chemical properties were characterized. Interestingly, hydrogel PVA-HLC-T80 and PVA-HLC-CS-T80 presented excellent swelling ratios, bacterial barrier activity, moisture vapor permeability, hemostasis activity and biocompatibility. Furthermore, in vivo evaluation of the healing capacity of these two hydrogels was checked by creating a full-thickness wound defect (1.3 cm × 1.3 cm) in rabbit. Macroscopic observation and subsequent hematoxylin eosin staining (H&E) staining and transmission electron microscopy (TEM) analysis at regular time intervals for 18 days revealed that the hydrogels significantly enhanced wound healing by reducing inflammation, promoting granulation tissue formation, collagen deposition and accelerating re-epithelialization. Taken together, the obtained data strongly encourage the use of these multifunctional hydrogels for skin wound dressings. MDPI 2017-12-18 /pmc/articles/PMC6418977/ /pubmed/30966027 http://dx.doi.org/10.3390/polym9120727 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pan, Hong
Fan, Daidi
Cao, Wei
Zhu, Chenhui
Duan, Zhiguang
Fu, Rongzhan
Li, Xian
Ma, Xiaoxuan
Preparation and Characterization of Breathable Hemostatic Hydrogel Dressings and Determination of Their Effects on Full-Thickness Defects
title Preparation and Characterization of Breathable Hemostatic Hydrogel Dressings and Determination of Their Effects on Full-Thickness Defects
title_full Preparation and Characterization of Breathable Hemostatic Hydrogel Dressings and Determination of Their Effects on Full-Thickness Defects
title_fullStr Preparation and Characterization of Breathable Hemostatic Hydrogel Dressings and Determination of Their Effects on Full-Thickness Defects
title_full_unstemmed Preparation and Characterization of Breathable Hemostatic Hydrogel Dressings and Determination of Their Effects on Full-Thickness Defects
title_short Preparation and Characterization of Breathable Hemostatic Hydrogel Dressings and Determination of Their Effects on Full-Thickness Defects
title_sort preparation and characterization of breathable hemostatic hydrogel dressings and determination of their effects on full-thickness defects
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6418977/
https://www.ncbi.nlm.nih.gov/pubmed/30966027
http://dx.doi.org/10.3390/polym9120727
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