The Multi-Template Molecularly Imprinted Polymer Based on SBA-15 for Selective Separation and Determination of Panax notoginseng Saponins Simultaneously in Biological Samples

The feasible, reliable and selective multi-template molecularly imprinted polymers (MT-MIPs) based on SBA-15 (SBA-15@MT-MIPs) for the selective separation and determination of the trace level of ginsenoside Rb(1) (Rb(1)), ginsenoside Rg(1) (Rg(1)) and notoginsenoside R(1) (R(1)) simultaneously from biological samples were developed. The polymers were constructed by SBA-15 as support, Rb(1), Rg(1), R(1) as multi-template, acrylamide (AM) as functional monomer and ethylene glycol dimethacrylate (EGDMA) as cross-linker. The new synthetic SBA-15@MT-MIPs were satisfactorily applied to solid-phase extraction (SPE) coupled with high performance liquid chromatography (HPLC) for the separation and determination of trace Rb(1), Rg(1) and R(1) in plasma samples. Under the optimized conditions, the limits of detection (LODs) and quantitation (LOQs) of the proposed method for Rb(1), Rg(1) and R(1) were in the range of 0.63–0.75 ng·mL(−1) and 2.1–2.5 ng·mL(−1), respectively. The recoveries of R(1), Rb(1) and Rg(1) were obtained between 93.4% and 104.3% with relative standard deviations (RSDs) in the range of 3.3–4.2%. All results show that the obtained SBA-15@MT-MIPs could be a promising prospect for the practical application in the selective separation and enrichment of trace Panax notoginseng saponins (PNS) in the biological samples.