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Preparation of a Cross-Linked Cartilage Acellular-Matrix Film and Its In Vivo Evaluation as an Antiadhesive Barrier

In this paper, a cartilage acellular-matrix (CAM) is chosen as a biomaterial for an effective antiadhesive barrier to apply between injured tissue and healthy tissues or organs. CAM is cross-linked using glutaraldehyde to create a cross-linked CAM (Cx-CAM) film. Cx-CAM has higher elastic modulus and...

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Autores principales: Park, Joon Yeong, Song, Bo Ram, Lee, Jin Woo, Park, Seung Hun, Kang, Tae Woong, Yun, Hee-Woong, Park, Sang-Hyug, Min, Byoung Hyun, Kim, Moon Suk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6419041/
https://www.ncbi.nlm.nih.gov/pubmed/30960232
http://dx.doi.org/10.3390/polym11020247
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author Park, Joon Yeong
Song, Bo Ram
Lee, Jin Woo
Park, Seung Hun
Kang, Tae Woong
Yun, Hee-Woong
Park, Sang-Hyug
Min, Byoung Hyun
Kim, Moon Suk
author_facet Park, Joon Yeong
Song, Bo Ram
Lee, Jin Woo
Park, Seung Hun
Kang, Tae Woong
Yun, Hee-Woong
Park, Sang-Hyug
Min, Byoung Hyun
Kim, Moon Suk
author_sort Park, Joon Yeong
collection PubMed
description In this paper, a cartilage acellular-matrix (CAM) is chosen as a biomaterial for an effective antiadhesive barrier to apply between injured tissue and healthy tissues or organs. CAM is cross-linked using glutaraldehyde to create a cross-linked CAM (Cx-CAM) film. Cx-CAM has higher elastic modulus and toughness and more hydrophobic surface properties than CAM before cross-linking. Small intestinal submucosa (SIS), cross-linked SIS (Cx-SIS) as a negative control, and Seprafilm as a positive control are used in an experiment as adhesion barriers. Human umbilical vein endothelial cells (HUVECs) on SIS, Cx-SIS, or in a culture plate get attached and effectively proliferate for 7 days, but Cx-CAM and Seprafilm allow for little or no attachment and proliferation of HUVECs, thus manifesting antiadhesive and antiproliferative effects. In animals with surgical damage to the peritoneal wall and cecum, Cx-CAM and Seprafilm afford little adhesion and negligible inflammation after seven days, as confirmed by hematoxylin and eosin staining and macrophage staining, in contrast to an untreated-injury model, SIS, or Cx-SIS film. Cx-CAM significantly suppresses the formation of blood vessels between the peritoneal wall and cecum, as confirmed by CD31 staining. Overall, the newly designed Cx-CAM film works well as an antiadhesion barrier and has better anti-tissue adhesion efficiency.
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spelling pubmed-64190412019-04-02 Preparation of a Cross-Linked Cartilage Acellular-Matrix Film and Its In Vivo Evaluation as an Antiadhesive Barrier Park, Joon Yeong Song, Bo Ram Lee, Jin Woo Park, Seung Hun Kang, Tae Woong Yun, Hee-Woong Park, Sang-Hyug Min, Byoung Hyun Kim, Moon Suk Polymers (Basel) Article In this paper, a cartilage acellular-matrix (CAM) is chosen as a biomaterial for an effective antiadhesive barrier to apply between injured tissue and healthy tissues or organs. CAM is cross-linked using glutaraldehyde to create a cross-linked CAM (Cx-CAM) film. Cx-CAM has higher elastic modulus and toughness and more hydrophobic surface properties than CAM before cross-linking. Small intestinal submucosa (SIS), cross-linked SIS (Cx-SIS) as a negative control, and Seprafilm as a positive control are used in an experiment as adhesion barriers. Human umbilical vein endothelial cells (HUVECs) on SIS, Cx-SIS, or in a culture plate get attached and effectively proliferate for 7 days, but Cx-CAM and Seprafilm allow for little or no attachment and proliferation of HUVECs, thus manifesting antiadhesive and antiproliferative effects. In animals with surgical damage to the peritoneal wall and cecum, Cx-CAM and Seprafilm afford little adhesion and negligible inflammation after seven days, as confirmed by hematoxylin and eosin staining and macrophage staining, in contrast to an untreated-injury model, SIS, or Cx-SIS film. Cx-CAM significantly suppresses the formation of blood vessels between the peritoneal wall and cecum, as confirmed by CD31 staining. Overall, the newly designed Cx-CAM film works well as an antiadhesion barrier and has better anti-tissue adhesion efficiency. MDPI 2019-02-02 /pmc/articles/PMC6419041/ /pubmed/30960232 http://dx.doi.org/10.3390/polym11020247 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Park, Joon Yeong
Song, Bo Ram
Lee, Jin Woo
Park, Seung Hun
Kang, Tae Woong
Yun, Hee-Woong
Park, Sang-Hyug
Min, Byoung Hyun
Kim, Moon Suk
Preparation of a Cross-Linked Cartilage Acellular-Matrix Film and Its In Vivo Evaluation as an Antiadhesive Barrier
title Preparation of a Cross-Linked Cartilage Acellular-Matrix Film and Its In Vivo Evaluation as an Antiadhesive Barrier
title_full Preparation of a Cross-Linked Cartilage Acellular-Matrix Film and Its In Vivo Evaluation as an Antiadhesive Barrier
title_fullStr Preparation of a Cross-Linked Cartilage Acellular-Matrix Film and Its In Vivo Evaluation as an Antiadhesive Barrier
title_full_unstemmed Preparation of a Cross-Linked Cartilage Acellular-Matrix Film and Its In Vivo Evaluation as an Antiadhesive Barrier
title_short Preparation of a Cross-Linked Cartilage Acellular-Matrix Film and Its In Vivo Evaluation as an Antiadhesive Barrier
title_sort preparation of a cross-linked cartilage acellular-matrix film and its in vivo evaluation as an antiadhesive barrier
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6419041/
https://www.ncbi.nlm.nih.gov/pubmed/30960232
http://dx.doi.org/10.3390/polym11020247
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