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Structural basis of guanine nucleotide exchange for Rab11 by SH3BP5

The Rab GTPase family is a major regulator of membrane traffic in eukaryotic cells. The Rab11 subfamily plays important roles in specific trafficking events such as exocytosis, endosomal recycling, and cytokinesis. SH3BP5 and SH3BP5-like (SH3BP5L) proteins have recently been found to serve as guanin...

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Autores principales: Goto-Ito, Sakurako, Morooka, Nobukatsu, Yamagata, Atsushi, Sato, Yusuke, Sato, Ken, Fukai, Shuya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Life Science Alliance LLC 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6419104/
https://www.ncbi.nlm.nih.gov/pubmed/30872413
http://dx.doi.org/10.26508/lsa.201900297
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author Goto-Ito, Sakurako
Morooka, Nobukatsu
Yamagata, Atsushi
Sato, Yusuke
Sato, Ken
Fukai, Shuya
author_facet Goto-Ito, Sakurako
Morooka, Nobukatsu
Yamagata, Atsushi
Sato, Yusuke
Sato, Ken
Fukai, Shuya
author_sort Goto-Ito, Sakurako
collection PubMed
description The Rab GTPase family is a major regulator of membrane traffic in eukaryotic cells. The Rab11 subfamily plays important roles in specific trafficking events such as exocytosis, endosomal recycling, and cytokinesis. SH3BP5 and SH3BP5-like (SH3BP5L) proteins have recently been found to serve as guanine nucleotide exchange factors (GEF) for Rab11. Here, we report the crystal structures of the SH3BP5 GEF domain alone and its complex with Rab11a. SH3BP5 exhibits a V-shaped structure comprising two coiled coils. The coiled coil composed of α1, and α4 is solely responsible for the Rab11a binding and GEF activity. SH3BP5 pulls out and deforms switch I of Rab11a so as to facilitate the GDP release from Rab11a. SH3BP5 interacts with the N-terminal region, switch I, interswitch, and switch II of Rab11a. SH3BP5 and SH3BP5L localize to Rab11-positive recycling endosomes and show GEF activity for all of the Rab11 family but not for Rab14. Fluorescence-based GEF assays combined with site-directed mutagenesis reveal the essential interactions between SH3BP5 and Rab11 family proteins for the GEF reaction on recycling endosomes.
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spelling pubmed-64191042019-03-15 Structural basis of guanine nucleotide exchange for Rab11 by SH3BP5 Goto-Ito, Sakurako Morooka, Nobukatsu Yamagata, Atsushi Sato, Yusuke Sato, Ken Fukai, Shuya Life Sci Alliance Research Articles The Rab GTPase family is a major regulator of membrane traffic in eukaryotic cells. The Rab11 subfamily plays important roles in specific trafficking events such as exocytosis, endosomal recycling, and cytokinesis. SH3BP5 and SH3BP5-like (SH3BP5L) proteins have recently been found to serve as guanine nucleotide exchange factors (GEF) for Rab11. Here, we report the crystal structures of the SH3BP5 GEF domain alone and its complex with Rab11a. SH3BP5 exhibits a V-shaped structure comprising two coiled coils. The coiled coil composed of α1, and α4 is solely responsible for the Rab11a binding and GEF activity. SH3BP5 pulls out and deforms switch I of Rab11a so as to facilitate the GDP release from Rab11a. SH3BP5 interacts with the N-terminal region, switch I, interswitch, and switch II of Rab11a. SH3BP5 and SH3BP5L localize to Rab11-positive recycling endosomes and show GEF activity for all of the Rab11 family but not for Rab14. Fluorescence-based GEF assays combined with site-directed mutagenesis reveal the essential interactions between SH3BP5 and Rab11 family proteins for the GEF reaction on recycling endosomes. Life Science Alliance LLC 2019-03-14 /pmc/articles/PMC6419104/ /pubmed/30872413 http://dx.doi.org/10.26508/lsa.201900297 Text en © 2019 Goto-Ito et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).
spellingShingle Research Articles
Goto-Ito, Sakurako
Morooka, Nobukatsu
Yamagata, Atsushi
Sato, Yusuke
Sato, Ken
Fukai, Shuya
Structural basis of guanine nucleotide exchange for Rab11 by SH3BP5
title Structural basis of guanine nucleotide exchange for Rab11 by SH3BP5
title_full Structural basis of guanine nucleotide exchange for Rab11 by SH3BP5
title_fullStr Structural basis of guanine nucleotide exchange for Rab11 by SH3BP5
title_full_unstemmed Structural basis of guanine nucleotide exchange for Rab11 by SH3BP5
title_short Structural basis of guanine nucleotide exchange for Rab11 by SH3BP5
title_sort structural basis of guanine nucleotide exchange for rab11 by sh3bp5
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6419104/
https://www.ncbi.nlm.nih.gov/pubmed/30872413
http://dx.doi.org/10.26508/lsa.201900297
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