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Temperature Responsive Nanoparticles Based on PEGylated Polyaspartamide Derivatives for Drug Delivery
The temperature responsive PEGylated polyaspartamide derivative, denoted as mPEG-PAAHP, was synthesized by the click reaction. FTIR and (1)H NMR were adopted to characterize and confirm the chemical structures of the obtained mPEG-PAAHPs. The temperature responsive behavior investigated by transmitt...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6419189/ https://www.ncbi.nlm.nih.gov/pubmed/30960299 http://dx.doi.org/10.3390/polym11020316 |
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author | Zhang, Guangyan Jiang, Xulin |
author_facet | Zhang, Guangyan Jiang, Xulin |
author_sort | Zhang, Guangyan |
collection | PubMed |
description | The temperature responsive PEGylated polyaspartamide derivative, denoted as mPEG-PAAHP, was synthesized by the click reaction. FTIR and (1)H NMR were adopted to characterize and confirm the chemical structures of the obtained mPEG-PAAHPs. The temperature responsive behavior investigated by transmittance and dynamic light scattering showed that some of the obtained mPEG-PAAHPs exhibited obvious temperature responsiveness and could be used to prepare nanoparticles by quickly heating. Drug paclitaxel can be encapsulated into mPEG-PAAHP based nanoparticles with a high encapsulation efficiency up to 99% (corresponding to a drug loading content of around 9.9%). Dynamic light scattering results showed that the PTX-loaded nanoparticles had a mean size around 80 nm (PDI<0.2) and good stability in PBS with 150 mM ionic strength. In vitro cytotoxicity results showed that mPEG-PAAHP did not show any toxicity to HeLa cells, but the PTX-loaded nanoparticles based on mPEG-PAAHP exhibited obvious anti-cancer activity. Thus, the temperature responsive PEGylated polyaspartamide derivative mPEG-PAAHP may be a promising drug delivery system. |
format | Online Article Text |
id | pubmed-6419189 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-64191892019-04-02 Temperature Responsive Nanoparticles Based on PEGylated Polyaspartamide Derivatives for Drug Delivery Zhang, Guangyan Jiang, Xulin Polymers (Basel) Article The temperature responsive PEGylated polyaspartamide derivative, denoted as mPEG-PAAHP, was synthesized by the click reaction. FTIR and (1)H NMR were adopted to characterize and confirm the chemical structures of the obtained mPEG-PAAHPs. The temperature responsive behavior investigated by transmittance and dynamic light scattering showed that some of the obtained mPEG-PAAHPs exhibited obvious temperature responsiveness and could be used to prepare nanoparticles by quickly heating. Drug paclitaxel can be encapsulated into mPEG-PAAHP based nanoparticles with a high encapsulation efficiency up to 99% (corresponding to a drug loading content of around 9.9%). Dynamic light scattering results showed that the PTX-loaded nanoparticles had a mean size around 80 nm (PDI<0.2) and good stability in PBS with 150 mM ionic strength. In vitro cytotoxicity results showed that mPEG-PAAHP did not show any toxicity to HeLa cells, but the PTX-loaded nanoparticles based on mPEG-PAAHP exhibited obvious anti-cancer activity. Thus, the temperature responsive PEGylated polyaspartamide derivative mPEG-PAAHP may be a promising drug delivery system. MDPI 2019-02-13 /pmc/articles/PMC6419189/ /pubmed/30960299 http://dx.doi.org/10.3390/polym11020316 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhang, Guangyan Jiang, Xulin Temperature Responsive Nanoparticles Based on PEGylated Polyaspartamide Derivatives for Drug Delivery |
title | Temperature Responsive Nanoparticles Based on PEGylated Polyaspartamide Derivatives for Drug Delivery |
title_full | Temperature Responsive Nanoparticles Based on PEGylated Polyaspartamide Derivatives for Drug Delivery |
title_fullStr | Temperature Responsive Nanoparticles Based on PEGylated Polyaspartamide Derivatives for Drug Delivery |
title_full_unstemmed | Temperature Responsive Nanoparticles Based on PEGylated Polyaspartamide Derivatives for Drug Delivery |
title_short | Temperature Responsive Nanoparticles Based on PEGylated Polyaspartamide Derivatives for Drug Delivery |
title_sort | temperature responsive nanoparticles based on pegylated polyaspartamide derivatives for drug delivery |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6419189/ https://www.ncbi.nlm.nih.gov/pubmed/30960299 http://dx.doi.org/10.3390/polym11020316 |
work_keys_str_mv | AT zhangguangyan temperatureresponsivenanoparticlesbasedonpegylatedpolyaspartamidederivativesfordrugdelivery AT jiangxulin temperatureresponsivenanoparticlesbasedonpegylatedpolyaspartamidederivativesfordrugdelivery |