Plasma cell-free DNA: a potential biomarker for early prediction of severe dengue

BACKGROUND: Considerable progress has been made in dengue management, however the lack of appropriate predictors of severity has led to huge number of unwanted admissions mostly decided on the grounds of warning signs. Apoptosis related mediators, among others, are known to correlate with severe den...

Descripción completa

Detalles Bibliográficos
Autores principales: Phuong, Nguyen Thi Ngoc, Manh, Dao Huy, Dumre, Shyam Prakash, Mizukami, Shusaku, Weiss, Lan Nguyen, Van Thuong, Nguyen, Ha, Tran Thi Ngoc, Phuc, Le Hong, Van An, Tran, Tieu, Thuan Minh, Kamel, Mohamed Gomaa, Morra, Mostafa Ebraheem, Huong, Vu Thi Que, Huy, Nguyen Tien, Hirayama, Kenji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6419393/
https://www.ncbi.nlm.nih.gov/pubmed/30871553
http://dx.doi.org/10.1186/s12941-019-0309-x
_version_ 1783403935025856512
author Phuong, Nguyen Thi Ngoc
Manh, Dao Huy
Dumre, Shyam Prakash
Mizukami, Shusaku
Weiss, Lan Nguyen
Van Thuong, Nguyen
Ha, Tran Thi Ngoc
Phuc, Le Hong
Van An, Tran
Tieu, Thuan Minh
Kamel, Mohamed Gomaa
Morra, Mostafa Ebraheem
Huong, Vu Thi Que
Huy, Nguyen Tien
Hirayama, Kenji
author_facet Phuong, Nguyen Thi Ngoc
Manh, Dao Huy
Dumre, Shyam Prakash
Mizukami, Shusaku
Weiss, Lan Nguyen
Van Thuong, Nguyen
Ha, Tran Thi Ngoc
Phuc, Le Hong
Van An, Tran
Tieu, Thuan Minh
Kamel, Mohamed Gomaa
Morra, Mostafa Ebraheem
Huong, Vu Thi Que
Huy, Nguyen Tien
Hirayama, Kenji
author_sort Phuong, Nguyen Thi Ngoc
collection PubMed
description BACKGROUND: Considerable progress has been made in dengue management, however the lack of appropriate predictors of severity has led to huge number of unwanted admissions mostly decided on the grounds of warning signs. Apoptosis related mediators, among others, are known to correlate with severe dengue (SD) although no predictive validity is established. The objective of this study was to investigate the association of plasma cell-free DNA (cfDNA) with SD, and evaluate its prognostic value in SD prediction at acute phase. METHODS: This was a hospital-based prospective cohort study conducted in Vietnam. All the recruited patients were required to be admitted to the hospital and were strictly monitored for various laboratory and clinical parameters (including progression to SD) until discharged. Plasma samples collected during acute phase (6–48 h before defervescence) were used to estimate the level of cfDNA. RESULTS: Of the 61 dengue patients, SD patients (n = 8) developed shock syndrome in 4.8 days (95% CI 3.7–5.4) after the fever onset. Plasma cfDNA levels before the defervescence of SD patients were significantly higher than the non-SD group (p = 0.0493). From the receiver operating characteristic (ROC) curve analysis, a cut-off of > 36.9 ng/mL was able to predict SD with a good sensitivity (87.5%), specificity (54.7%), and area under the curve (AUC) (0.72, 95% CI 0.55–0.88; p = 0.0493). CONCLUSIONS: Taken together, these findings suggest that cfDNA could serve as a potential prognostic biomarker of SD. Studies with cfDNA kinetics and its combination with other biomarkers and clinical parameters would further improve the diagnostic ability for SD. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12941-019-0309-x) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-6419393
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-64193932019-03-27 Plasma cell-free DNA: a potential biomarker for early prediction of severe dengue Phuong, Nguyen Thi Ngoc Manh, Dao Huy Dumre, Shyam Prakash Mizukami, Shusaku Weiss, Lan Nguyen Van Thuong, Nguyen Ha, Tran Thi Ngoc Phuc, Le Hong Van An, Tran Tieu, Thuan Minh Kamel, Mohamed Gomaa Morra, Mostafa Ebraheem Huong, Vu Thi Que Huy, Nguyen Tien Hirayama, Kenji Ann Clin Microbiol Antimicrob Research BACKGROUND: Considerable progress has been made in dengue management, however the lack of appropriate predictors of severity has led to huge number of unwanted admissions mostly decided on the grounds of warning signs. Apoptosis related mediators, among others, are known to correlate with severe dengue (SD) although no predictive validity is established. The objective of this study was to investigate the association of plasma cell-free DNA (cfDNA) with SD, and evaluate its prognostic value in SD prediction at acute phase. METHODS: This was a hospital-based prospective cohort study conducted in Vietnam. All the recruited patients were required to be admitted to the hospital and were strictly monitored for various laboratory and clinical parameters (including progression to SD) until discharged. Plasma samples collected during acute phase (6–48 h before defervescence) were used to estimate the level of cfDNA. RESULTS: Of the 61 dengue patients, SD patients (n = 8) developed shock syndrome in 4.8 days (95% CI 3.7–5.4) after the fever onset. Plasma cfDNA levels before the defervescence of SD patients were significantly higher than the non-SD group (p = 0.0493). From the receiver operating characteristic (ROC) curve analysis, a cut-off of > 36.9 ng/mL was able to predict SD with a good sensitivity (87.5%), specificity (54.7%), and area under the curve (AUC) (0.72, 95% CI 0.55–0.88; p = 0.0493). CONCLUSIONS: Taken together, these findings suggest that cfDNA could serve as a potential prognostic biomarker of SD. Studies with cfDNA kinetics and its combination with other biomarkers and clinical parameters would further improve the diagnostic ability for SD. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12941-019-0309-x) contains supplementary material, which is available to authorized users. BioMed Central 2019-03-13 /pmc/articles/PMC6419393/ /pubmed/30871553 http://dx.doi.org/10.1186/s12941-019-0309-x Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Phuong, Nguyen Thi Ngoc
Manh, Dao Huy
Dumre, Shyam Prakash
Mizukami, Shusaku
Weiss, Lan Nguyen
Van Thuong, Nguyen
Ha, Tran Thi Ngoc
Phuc, Le Hong
Van An, Tran
Tieu, Thuan Minh
Kamel, Mohamed Gomaa
Morra, Mostafa Ebraheem
Huong, Vu Thi Que
Huy, Nguyen Tien
Hirayama, Kenji
Plasma cell-free DNA: a potential biomarker for early prediction of severe dengue
title Plasma cell-free DNA: a potential biomarker for early prediction of severe dengue
title_full Plasma cell-free DNA: a potential biomarker for early prediction of severe dengue
title_fullStr Plasma cell-free DNA: a potential biomarker for early prediction of severe dengue
title_full_unstemmed Plasma cell-free DNA: a potential biomarker for early prediction of severe dengue
title_short Plasma cell-free DNA: a potential biomarker for early prediction of severe dengue
title_sort plasma cell-free dna: a potential biomarker for early prediction of severe dengue
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6419393/
https://www.ncbi.nlm.nih.gov/pubmed/30871553
http://dx.doi.org/10.1186/s12941-019-0309-x
work_keys_str_mv AT phuongnguyenthingoc plasmacellfreednaapotentialbiomarkerforearlypredictionofseveredengue
AT manhdaohuy plasmacellfreednaapotentialbiomarkerforearlypredictionofseveredengue
AT dumreshyamprakash plasmacellfreednaapotentialbiomarkerforearlypredictionofseveredengue
AT mizukamishusaku plasmacellfreednaapotentialbiomarkerforearlypredictionofseveredengue
AT weisslannguyen plasmacellfreednaapotentialbiomarkerforearlypredictionofseveredengue
AT vanthuongnguyen plasmacellfreednaapotentialbiomarkerforearlypredictionofseveredengue
AT hatranthingoc plasmacellfreednaapotentialbiomarkerforearlypredictionofseveredengue
AT phuclehong plasmacellfreednaapotentialbiomarkerforearlypredictionofseveredengue
AT vanantran plasmacellfreednaapotentialbiomarkerforearlypredictionofseveredengue
AT tieuthuanminh plasmacellfreednaapotentialbiomarkerforearlypredictionofseveredengue
AT kamelmohamedgomaa plasmacellfreednaapotentialbiomarkerforearlypredictionofseveredengue
AT morramostafaebraheem plasmacellfreednaapotentialbiomarkerforearlypredictionofseveredengue
AT huongvuthique plasmacellfreednaapotentialbiomarkerforearlypredictionofseveredengue
AT huynguyentien plasmacellfreednaapotentialbiomarkerforearlypredictionofseveredengue
AT hirayamakenji plasmacellfreednaapotentialbiomarkerforearlypredictionofseveredengue