Plasma cell-free DNA: a potential biomarker for early prediction of severe dengue
BACKGROUND: Considerable progress has been made in dengue management, however the lack of appropriate predictors of severity has led to huge number of unwanted admissions mostly decided on the grounds of warning signs. Apoptosis related mediators, among others, are known to correlate with severe den...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6419393/ https://www.ncbi.nlm.nih.gov/pubmed/30871553 http://dx.doi.org/10.1186/s12941-019-0309-x |
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author | Phuong, Nguyen Thi Ngoc Manh, Dao Huy Dumre, Shyam Prakash Mizukami, Shusaku Weiss, Lan Nguyen Van Thuong, Nguyen Ha, Tran Thi Ngoc Phuc, Le Hong Van An, Tran Tieu, Thuan Minh Kamel, Mohamed Gomaa Morra, Mostafa Ebraheem Huong, Vu Thi Que Huy, Nguyen Tien Hirayama, Kenji |
author_facet | Phuong, Nguyen Thi Ngoc Manh, Dao Huy Dumre, Shyam Prakash Mizukami, Shusaku Weiss, Lan Nguyen Van Thuong, Nguyen Ha, Tran Thi Ngoc Phuc, Le Hong Van An, Tran Tieu, Thuan Minh Kamel, Mohamed Gomaa Morra, Mostafa Ebraheem Huong, Vu Thi Que Huy, Nguyen Tien Hirayama, Kenji |
author_sort | Phuong, Nguyen Thi Ngoc |
collection | PubMed |
description | BACKGROUND: Considerable progress has been made in dengue management, however the lack of appropriate predictors of severity has led to huge number of unwanted admissions mostly decided on the grounds of warning signs. Apoptosis related mediators, among others, are known to correlate with severe dengue (SD) although no predictive validity is established. The objective of this study was to investigate the association of plasma cell-free DNA (cfDNA) with SD, and evaluate its prognostic value in SD prediction at acute phase. METHODS: This was a hospital-based prospective cohort study conducted in Vietnam. All the recruited patients were required to be admitted to the hospital and were strictly monitored for various laboratory and clinical parameters (including progression to SD) until discharged. Plasma samples collected during acute phase (6–48 h before defervescence) were used to estimate the level of cfDNA. RESULTS: Of the 61 dengue patients, SD patients (n = 8) developed shock syndrome in 4.8 days (95% CI 3.7–5.4) after the fever onset. Plasma cfDNA levels before the defervescence of SD patients were significantly higher than the non-SD group (p = 0.0493). From the receiver operating characteristic (ROC) curve analysis, a cut-off of > 36.9 ng/mL was able to predict SD with a good sensitivity (87.5%), specificity (54.7%), and area under the curve (AUC) (0.72, 95% CI 0.55–0.88; p = 0.0493). CONCLUSIONS: Taken together, these findings suggest that cfDNA could serve as a potential prognostic biomarker of SD. Studies with cfDNA kinetics and its combination with other biomarkers and clinical parameters would further improve the diagnostic ability for SD. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12941-019-0309-x) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6419393 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-64193932019-03-27 Plasma cell-free DNA: a potential biomarker for early prediction of severe dengue Phuong, Nguyen Thi Ngoc Manh, Dao Huy Dumre, Shyam Prakash Mizukami, Shusaku Weiss, Lan Nguyen Van Thuong, Nguyen Ha, Tran Thi Ngoc Phuc, Le Hong Van An, Tran Tieu, Thuan Minh Kamel, Mohamed Gomaa Morra, Mostafa Ebraheem Huong, Vu Thi Que Huy, Nguyen Tien Hirayama, Kenji Ann Clin Microbiol Antimicrob Research BACKGROUND: Considerable progress has been made in dengue management, however the lack of appropriate predictors of severity has led to huge number of unwanted admissions mostly decided on the grounds of warning signs. Apoptosis related mediators, among others, are known to correlate with severe dengue (SD) although no predictive validity is established. The objective of this study was to investigate the association of plasma cell-free DNA (cfDNA) with SD, and evaluate its prognostic value in SD prediction at acute phase. METHODS: This was a hospital-based prospective cohort study conducted in Vietnam. All the recruited patients were required to be admitted to the hospital and were strictly monitored for various laboratory and clinical parameters (including progression to SD) until discharged. Plasma samples collected during acute phase (6–48 h before defervescence) were used to estimate the level of cfDNA. RESULTS: Of the 61 dengue patients, SD patients (n = 8) developed shock syndrome in 4.8 days (95% CI 3.7–5.4) after the fever onset. Plasma cfDNA levels before the defervescence of SD patients were significantly higher than the non-SD group (p = 0.0493). From the receiver operating characteristic (ROC) curve analysis, a cut-off of > 36.9 ng/mL was able to predict SD with a good sensitivity (87.5%), specificity (54.7%), and area under the curve (AUC) (0.72, 95% CI 0.55–0.88; p = 0.0493). CONCLUSIONS: Taken together, these findings suggest that cfDNA could serve as a potential prognostic biomarker of SD. Studies with cfDNA kinetics and its combination with other biomarkers and clinical parameters would further improve the diagnostic ability for SD. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12941-019-0309-x) contains supplementary material, which is available to authorized users. BioMed Central 2019-03-13 /pmc/articles/PMC6419393/ /pubmed/30871553 http://dx.doi.org/10.1186/s12941-019-0309-x Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Phuong, Nguyen Thi Ngoc Manh, Dao Huy Dumre, Shyam Prakash Mizukami, Shusaku Weiss, Lan Nguyen Van Thuong, Nguyen Ha, Tran Thi Ngoc Phuc, Le Hong Van An, Tran Tieu, Thuan Minh Kamel, Mohamed Gomaa Morra, Mostafa Ebraheem Huong, Vu Thi Que Huy, Nguyen Tien Hirayama, Kenji Plasma cell-free DNA: a potential biomarker for early prediction of severe dengue |
title | Plasma cell-free DNA: a potential biomarker for early prediction of severe dengue |
title_full | Plasma cell-free DNA: a potential biomarker for early prediction of severe dengue |
title_fullStr | Plasma cell-free DNA: a potential biomarker for early prediction of severe dengue |
title_full_unstemmed | Plasma cell-free DNA: a potential biomarker for early prediction of severe dengue |
title_short | Plasma cell-free DNA: a potential biomarker for early prediction of severe dengue |
title_sort | plasma cell-free dna: a potential biomarker for early prediction of severe dengue |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6419393/ https://www.ncbi.nlm.nih.gov/pubmed/30871553 http://dx.doi.org/10.1186/s12941-019-0309-x |
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