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Development and evaluation of novel salt‐tolerant Eucalyptus trees by molecular breeding using an RNA‐Binding‐Protein gene derived from common ice plant (Mesembryanthemum crystallinum L.)
The breeding of plantation forestry trees for the possible afforestation of marginal land would be one approach to addressing global warming issues. Here, we developed novel transgenic Eucalyptus trees (Eucalyptus camaldulensis Dehnh.) harbouring an RNA‐Binding‐Protein (McRBP) gene derived from a ha...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6419579/ https://www.ncbi.nlm.nih.gov/pubmed/30230168 http://dx.doi.org/10.1111/pbi.13016 |
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author | Tran, Ngoc‐Ha Thi Oguchi, Taichi Akatsuka, Nobuhumi Matsunaga, Etsuko Kawaoka, Akiyoshi Yamada, Akiyo Ozeki, Yoshihiro Watanabe, Kazuo N. Kikuchi, Akira |
author_facet | Tran, Ngoc‐Ha Thi Oguchi, Taichi Akatsuka, Nobuhumi Matsunaga, Etsuko Kawaoka, Akiyoshi Yamada, Akiyo Ozeki, Yoshihiro Watanabe, Kazuo N. Kikuchi, Akira |
author_sort | Tran, Ngoc‐Ha Thi |
collection | PubMed |
description | The breeding of plantation forestry trees for the possible afforestation of marginal land would be one approach to addressing global warming issues. Here, we developed novel transgenic Eucalyptus trees (Eucalyptus camaldulensis Dehnh.) harbouring an RNA‐Binding‐Protein (McRBP) gene derived from a halophyte plant, common ice plant (Mesembryanthemum crystallinum L.). We conducted screened‐house trials of the transgenic Eucalyptus using two different stringency salinity stress conditions to evaluate the plants’ acute and chronic salt stress tolerances. Treatment with 400 mM NaCl, as the high‐stringency salinity stress, resulted in soil electrical conductivity (EC) levels >20 mS/cm within 4 weeks. With the 400 mM NaCl treatment, >70% of the transgenic plants were intact, whereas >40% of the non‐transgenic plants were withered. Treatment with 70 mM NaCl, as the moderate‐stringency salinity stress, resulted in soil EC levels of approx. 9 mS/cm after 2 months, and these salinity levels were maintained for the next 4 months. All plants regardless of transgenic or non‐transgenic status survived the 70 mM NaCl treatment, but after 6‐month treatment the transgenic plants showed significantly higher growth and quantum yield of photosynthesis levels compared to the non‐transgenic plants. In addition, the salt accumulation in the leaves of the transgenic plants was 30% lower than that of non‐transgenic plants after 15‐week moderate salt stress treatment. There results suggest that McRBP expression in the transgenic Eucalyptus enhances their salt tolerance both acutely and chronically. |
format | Online Article Text |
id | pubmed-6419579 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-64195792019-03-18 Development and evaluation of novel salt‐tolerant Eucalyptus trees by molecular breeding using an RNA‐Binding‐Protein gene derived from common ice plant (Mesembryanthemum crystallinum L.) Tran, Ngoc‐Ha Thi Oguchi, Taichi Akatsuka, Nobuhumi Matsunaga, Etsuko Kawaoka, Akiyoshi Yamada, Akiyo Ozeki, Yoshihiro Watanabe, Kazuo N. Kikuchi, Akira Plant Biotechnol J Research Articles The breeding of plantation forestry trees for the possible afforestation of marginal land would be one approach to addressing global warming issues. Here, we developed novel transgenic Eucalyptus trees (Eucalyptus camaldulensis Dehnh.) harbouring an RNA‐Binding‐Protein (McRBP) gene derived from a halophyte plant, common ice plant (Mesembryanthemum crystallinum L.). We conducted screened‐house trials of the transgenic Eucalyptus using two different stringency salinity stress conditions to evaluate the plants’ acute and chronic salt stress tolerances. Treatment with 400 mM NaCl, as the high‐stringency salinity stress, resulted in soil electrical conductivity (EC) levels >20 mS/cm within 4 weeks. With the 400 mM NaCl treatment, >70% of the transgenic plants were intact, whereas >40% of the non‐transgenic plants were withered. Treatment with 70 mM NaCl, as the moderate‐stringency salinity stress, resulted in soil EC levels of approx. 9 mS/cm after 2 months, and these salinity levels were maintained for the next 4 months. All plants regardless of transgenic or non‐transgenic status survived the 70 mM NaCl treatment, but after 6‐month treatment the transgenic plants showed significantly higher growth and quantum yield of photosynthesis levels compared to the non‐transgenic plants. In addition, the salt accumulation in the leaves of the transgenic plants was 30% lower than that of non‐transgenic plants after 15‐week moderate salt stress treatment. There results suggest that McRBP expression in the transgenic Eucalyptus enhances their salt tolerance both acutely and chronically. John Wiley and Sons Inc. 2018-10-12 2019-04 /pmc/articles/PMC6419579/ /pubmed/30230168 http://dx.doi.org/10.1111/pbi.13016 Text en © 2018 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Tran, Ngoc‐Ha Thi Oguchi, Taichi Akatsuka, Nobuhumi Matsunaga, Etsuko Kawaoka, Akiyoshi Yamada, Akiyo Ozeki, Yoshihiro Watanabe, Kazuo N. Kikuchi, Akira Development and evaluation of novel salt‐tolerant Eucalyptus trees by molecular breeding using an RNA‐Binding‐Protein gene derived from common ice plant (Mesembryanthemum crystallinum L.) |
title | Development and evaluation of novel salt‐tolerant Eucalyptus trees by molecular breeding using an RNA‐Binding‐Protein gene derived from common ice plant (Mesembryanthemum crystallinum L.) |
title_full | Development and evaluation of novel salt‐tolerant Eucalyptus trees by molecular breeding using an RNA‐Binding‐Protein gene derived from common ice plant (Mesembryanthemum crystallinum L.) |
title_fullStr | Development and evaluation of novel salt‐tolerant Eucalyptus trees by molecular breeding using an RNA‐Binding‐Protein gene derived from common ice plant (Mesembryanthemum crystallinum L.) |
title_full_unstemmed | Development and evaluation of novel salt‐tolerant Eucalyptus trees by molecular breeding using an RNA‐Binding‐Protein gene derived from common ice plant (Mesembryanthemum crystallinum L.) |
title_short | Development and evaluation of novel salt‐tolerant Eucalyptus trees by molecular breeding using an RNA‐Binding‐Protein gene derived from common ice plant (Mesembryanthemum crystallinum L.) |
title_sort | development and evaluation of novel salt‐tolerant eucalyptus trees by molecular breeding using an rna‐binding‐protein gene derived from common ice plant (mesembryanthemum crystallinum l.) |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6419579/ https://www.ncbi.nlm.nih.gov/pubmed/30230168 http://dx.doi.org/10.1111/pbi.13016 |
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