Cargando…

Comparative proteomic analysis in serum of former uranium miners with and without radon induced squamous lung cancer

SUMMARY: Former uranium miners of the Wismut Company, East Germany, have been exposed to ionizing radiation from radon decay products and therefore were at high risk for lung cancer. Since histological types of cancer in the so called Wismut cohort revealed an association of high radon exposure with...

Descripción completa

Detalles Bibliográficos
Autores principales: Helmig, Simone, Lochnit, Günter, Schneider, Joachim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6419832/
https://www.ncbi.nlm.nih.gov/pubmed/30923558
http://dx.doi.org/10.1186/s12995-019-0228-y
_version_ 1783404006923567104
author Helmig, Simone
Lochnit, Günter
Schneider, Joachim
author_facet Helmig, Simone
Lochnit, Günter
Schneider, Joachim
author_sort Helmig, Simone
collection PubMed
description SUMMARY: Former uranium miners of the Wismut Company, East Germany, have been exposed to ionizing radiation from radon decay products and therefore were at high risk for lung cancer. Since histological types of cancer in the so called Wismut cohort revealed an association of high radon exposure with a higher relative frequency of squamous cell carcinoma (SqCC), we used comparative proteomic analysis to identify differentially expressed proteins in serum exposed uranium miners with SqCC. METHODE: Pooled sera of exposed former uranium miners without lung disease and pooled sera of former uranium miners with SqCC were analysed by 2-D gel electrophoresis. MALDI-TOF-MS was performed from reproducable, significantly, at least 5-fold up-regulated protein spots. Proteins were identified by MASCOT peptide mass fingerprint search. Additionally a receiver operating characteristic curve for CYFRA 21-1 was created. RESULTS: The protein spots were identified as Keratin 10 (K10), Keratin 1 (K1), complement factor H (CFH) and a haptoglobin (Hpt) fragment. The sensitivity for CYFRA 21-1 reveals 60% at a specifity of 95 and 80% at a specifity of 80%. Plotting the sensitivity against specifity reveals an AUC of 0.88. CONCLUSION: In SqCC Keratin 10 and 1 were strongly induced. This was associated with CYFRA 21-1, confirming the cytokeratin fragment as a tumormarker.
format Online
Article
Text
id pubmed-6419832
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-64198322019-03-28 Comparative proteomic analysis in serum of former uranium miners with and without radon induced squamous lung cancer Helmig, Simone Lochnit, Günter Schneider, Joachim J Occup Med Toxicol Research SUMMARY: Former uranium miners of the Wismut Company, East Germany, have been exposed to ionizing radiation from radon decay products and therefore were at high risk for lung cancer. Since histological types of cancer in the so called Wismut cohort revealed an association of high radon exposure with a higher relative frequency of squamous cell carcinoma (SqCC), we used comparative proteomic analysis to identify differentially expressed proteins in serum exposed uranium miners with SqCC. METHODE: Pooled sera of exposed former uranium miners without lung disease and pooled sera of former uranium miners with SqCC were analysed by 2-D gel electrophoresis. MALDI-TOF-MS was performed from reproducable, significantly, at least 5-fold up-regulated protein spots. Proteins were identified by MASCOT peptide mass fingerprint search. Additionally a receiver operating characteristic curve for CYFRA 21-1 was created. RESULTS: The protein spots were identified as Keratin 10 (K10), Keratin 1 (K1), complement factor H (CFH) and a haptoglobin (Hpt) fragment. The sensitivity for CYFRA 21-1 reveals 60% at a specifity of 95 and 80% at a specifity of 80%. Plotting the sensitivity against specifity reveals an AUC of 0.88. CONCLUSION: In SqCC Keratin 10 and 1 were strongly induced. This was associated with CYFRA 21-1, confirming the cytokeratin fragment as a tumormarker. BioMed Central 2019-03-15 /pmc/articles/PMC6419832/ /pubmed/30923558 http://dx.doi.org/10.1186/s12995-019-0228-y Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Helmig, Simone
Lochnit, Günter
Schneider, Joachim
Comparative proteomic analysis in serum of former uranium miners with and without radon induced squamous lung cancer
title Comparative proteomic analysis in serum of former uranium miners with and without radon induced squamous lung cancer
title_full Comparative proteomic analysis in serum of former uranium miners with and without radon induced squamous lung cancer
title_fullStr Comparative proteomic analysis in serum of former uranium miners with and without radon induced squamous lung cancer
title_full_unstemmed Comparative proteomic analysis in serum of former uranium miners with and without radon induced squamous lung cancer
title_short Comparative proteomic analysis in serum of former uranium miners with and without radon induced squamous lung cancer
title_sort comparative proteomic analysis in serum of former uranium miners with and without radon induced squamous lung cancer
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6419832/
https://www.ncbi.nlm.nih.gov/pubmed/30923558
http://dx.doi.org/10.1186/s12995-019-0228-y
work_keys_str_mv AT helmigsimone comparativeproteomicanalysisinserumofformeruraniumminerswithandwithoutradoninducedsquamouslungcancer
AT lochnitgunter comparativeproteomicanalysisinserumofformeruraniumminerswithandwithoutradoninducedsquamouslungcancer
AT schneiderjoachim comparativeproteomicanalysisinserumofformeruraniumminerswithandwithoutradoninducedsquamouslungcancer