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Limited relationships between reactive oxygen species levels in culture media and zygote and embryo development
PURPOSE: Reactive oxygen species (ROS) are thought to play a critical role in the success of IVF. The relationships between oxidative stress parameters in culture media and IVF outcomes have not been extensively investigated. The objective of this study is to examine the relationships between early...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6420490/ https://www.ncbi.nlm.nih.gov/pubmed/30415468 http://dx.doi.org/10.1007/s10815-018-1363-6 |
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author | Lan, Kuo-Chung Lin, Yi-Chi Chang, Yung-Chiao Lin, Hsin-Jung Tsai, Yi-Ru Kang, Hong-Yo |
author_facet | Lan, Kuo-Chung Lin, Yi-Chi Chang, Yung-Chiao Lin, Hsin-Jung Tsai, Yi-Ru Kang, Hong-Yo |
author_sort | Lan, Kuo-Chung |
collection | PubMed |
description | PURPOSE: Reactive oxygen species (ROS) are thought to play a critical role in the success of IVF. The relationships between oxidative stress parameters in culture media and IVF outcomes have not been extensively investigated. The objective of this study is to examine the relationships between early human embryonic parameters and levels of ROS in culture media. METHODS: This prospective study was conducted with 2633 spent culture media collected from patients undergoing conventional IVF (n = 101) and intracytoplasmic sperm injection (ICSI) (n = 60). Both fertilization and early culture were performed in universal IVF medium and G series medium. ROS levels were measured by chemiluminescence assays using luminol as the probe on days 1, 3, and 5 and determined the relationships of ROS levels with zygote condition, embryo quality, and clinical pregnancy rate. RESULTS: ROS levels per embryo in culture media on the corresponding days 1, 3, and 5 showed significant correlations between each pair in the total cohort. Similar results were observed in the IVF and ICSI groups, but day 1 and day 3 ROS levels were significantly higher in culture media of IVF than of ICSI embryos. ROS levels in culture medium were not significantly associated with embryo quality, blastocyst formation, or arrest. ROS levels on day 1 were similar in media of normally fertilized zygotes, unfertilized oocytes, and polyspermic zygotes and were not associated with delayed embryonic development, high fragmentation, blastocyst formation, or arrest after prolonged culture. ROS levels in media were not associated with the likelihood of conception. CONCLUSIONS: ROS levels in culture media may not be an effective indicator of embryo selection for IVF. |
format | Online Article Text |
id | pubmed-6420490 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-64204902019-04-03 Limited relationships between reactive oxygen species levels in culture media and zygote and embryo development Lan, Kuo-Chung Lin, Yi-Chi Chang, Yung-Chiao Lin, Hsin-Jung Tsai, Yi-Ru Kang, Hong-Yo J Assist Reprod Genet Embryo Biology PURPOSE: Reactive oxygen species (ROS) are thought to play a critical role in the success of IVF. The relationships between oxidative stress parameters in culture media and IVF outcomes have not been extensively investigated. The objective of this study is to examine the relationships between early human embryonic parameters and levels of ROS in culture media. METHODS: This prospective study was conducted with 2633 spent culture media collected from patients undergoing conventional IVF (n = 101) and intracytoplasmic sperm injection (ICSI) (n = 60). Both fertilization and early culture were performed in universal IVF medium and G series medium. ROS levels were measured by chemiluminescence assays using luminol as the probe on days 1, 3, and 5 and determined the relationships of ROS levels with zygote condition, embryo quality, and clinical pregnancy rate. RESULTS: ROS levels per embryo in culture media on the corresponding days 1, 3, and 5 showed significant correlations between each pair in the total cohort. Similar results were observed in the IVF and ICSI groups, but day 1 and day 3 ROS levels were significantly higher in culture media of IVF than of ICSI embryos. ROS levels in culture medium were not significantly associated with embryo quality, blastocyst formation, or arrest. ROS levels on day 1 were similar in media of normally fertilized zygotes, unfertilized oocytes, and polyspermic zygotes and were not associated with delayed embryonic development, high fragmentation, blastocyst formation, or arrest after prolonged culture. ROS levels in media were not associated with the likelihood of conception. CONCLUSIONS: ROS levels in culture media may not be an effective indicator of embryo selection for IVF. Springer US 2018-11-10 2019-02 /pmc/articles/PMC6420490/ /pubmed/30415468 http://dx.doi.org/10.1007/s10815-018-1363-6 Text en © The Author(s) 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Embryo Biology Lan, Kuo-Chung Lin, Yi-Chi Chang, Yung-Chiao Lin, Hsin-Jung Tsai, Yi-Ru Kang, Hong-Yo Limited relationships between reactive oxygen species levels in culture media and zygote and embryo development |
title | Limited relationships between reactive oxygen species levels in culture media and zygote and embryo development |
title_full | Limited relationships between reactive oxygen species levels in culture media and zygote and embryo development |
title_fullStr | Limited relationships between reactive oxygen species levels in culture media and zygote and embryo development |
title_full_unstemmed | Limited relationships between reactive oxygen species levels in culture media and zygote and embryo development |
title_short | Limited relationships between reactive oxygen species levels in culture media and zygote and embryo development |
title_sort | limited relationships between reactive oxygen species levels in culture media and zygote and embryo development |
topic | Embryo Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6420490/ https://www.ncbi.nlm.nih.gov/pubmed/30415468 http://dx.doi.org/10.1007/s10815-018-1363-6 |
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