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Deposition of Antibody Modified Upconversion Nanoparticles on Glass by a Laser-Assisted Method to Improve the Performance of Cell Culture
A suitable surface is vital for maintaining or even promoting cells’ function and communication. Recently, studies show that nanostructured coatings could have a potential in improving cell adhesion. However, it hardly minimizes the contamination by using traditional solution-coating technology. Mat...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6420592/ https://www.ncbi.nlm.nih.gov/pubmed/30877399 http://dx.doi.org/10.1186/s11671-019-2918-x |
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author | Yang, Songlin Tse, Wai Hei Zhang, Jin |
author_facet | Yang, Songlin Tse, Wai Hei Zhang, Jin |
author_sort | Yang, Songlin |
collection | PubMed |
description | A suitable surface is vital for maintaining or even promoting cells’ function and communication. Recently, studies show that nanostructured coatings could have a potential in improving cell adhesion. However, it hardly minimizes the contamination by using traditional solution-coating technology. Matrix-assisted pulsed laser evaporation (MAPLE) technique is a contamination-free process and demonstrates an efficient process to deposit biopolymer without damaging their backbone on the surface of various substrates. Here, upconversion nanoparticles (NaGdF(4): Yb(3+), Er(3+)) with/without immunoglobulin G (IgG) modification were produced by a one-pot synthesis method. The average size of the upconversion nanoparticles (UCNPs) is 50 ± 8 nm. IgG bio-conjugated on the surface of UCNPs can be directly observed by transmission electron microscope (TEM). MAPLE system utilizing a Nd:YAG laser (λ = 532 nm, ν = 10 Hz) is applied to deposit UCNPs with/without IgG modification on the glass bottom of culture dish. In addition, the behaviors of human umbilical vein endothelial cells (HUVECs) cultured on the culture dishes coated with UCNPs with/without IgG have been studied as compared to the control sample, glass coated with gelatin. No toxic effect is imposed on cells. The results of this work indicate that the deposition of UCNPs with/without antibody by the MAPLE technique could enhance the adhesion and proliferation of cells. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s11671-019-2918-x) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6420592 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-64205922019-04-05 Deposition of Antibody Modified Upconversion Nanoparticles on Glass by a Laser-Assisted Method to Improve the Performance of Cell Culture Yang, Songlin Tse, Wai Hei Zhang, Jin Nanoscale Res Lett Nano Express A suitable surface is vital for maintaining or even promoting cells’ function and communication. Recently, studies show that nanostructured coatings could have a potential in improving cell adhesion. However, it hardly minimizes the contamination by using traditional solution-coating technology. Matrix-assisted pulsed laser evaporation (MAPLE) technique is a contamination-free process and demonstrates an efficient process to deposit biopolymer without damaging their backbone on the surface of various substrates. Here, upconversion nanoparticles (NaGdF(4): Yb(3+), Er(3+)) with/without immunoglobulin G (IgG) modification were produced by a one-pot synthesis method. The average size of the upconversion nanoparticles (UCNPs) is 50 ± 8 nm. IgG bio-conjugated on the surface of UCNPs can be directly observed by transmission electron microscope (TEM). MAPLE system utilizing a Nd:YAG laser (λ = 532 nm, ν = 10 Hz) is applied to deposit UCNPs with/without IgG modification on the glass bottom of culture dish. In addition, the behaviors of human umbilical vein endothelial cells (HUVECs) cultured on the culture dishes coated with UCNPs with/without IgG have been studied as compared to the control sample, glass coated with gelatin. No toxic effect is imposed on cells. The results of this work indicate that the deposition of UCNPs with/without antibody by the MAPLE technique could enhance the adhesion and proliferation of cells. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s11671-019-2918-x) contains supplementary material, which is available to authorized users. Springer US 2019-03-15 /pmc/articles/PMC6420592/ /pubmed/30877399 http://dx.doi.org/10.1186/s11671-019-2918-x Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Nano Express Yang, Songlin Tse, Wai Hei Zhang, Jin Deposition of Antibody Modified Upconversion Nanoparticles on Glass by a Laser-Assisted Method to Improve the Performance of Cell Culture |
title | Deposition of Antibody Modified Upconversion Nanoparticles on Glass by a Laser-Assisted Method to Improve the Performance of Cell Culture |
title_full | Deposition of Antibody Modified Upconversion Nanoparticles on Glass by a Laser-Assisted Method to Improve the Performance of Cell Culture |
title_fullStr | Deposition of Antibody Modified Upconversion Nanoparticles on Glass by a Laser-Assisted Method to Improve the Performance of Cell Culture |
title_full_unstemmed | Deposition of Antibody Modified Upconversion Nanoparticles on Glass by a Laser-Assisted Method to Improve the Performance of Cell Culture |
title_short | Deposition of Antibody Modified Upconversion Nanoparticles on Glass by a Laser-Assisted Method to Improve the Performance of Cell Culture |
title_sort | deposition of antibody modified upconversion nanoparticles on glass by a laser-assisted method to improve the performance of cell culture |
topic | Nano Express |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6420592/ https://www.ncbi.nlm.nih.gov/pubmed/30877399 http://dx.doi.org/10.1186/s11671-019-2918-x |
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