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Definition of a cell surface signature for human cardiac progenitor cells after comprehensive comparative transcriptomic and proteomic characterization

Adult cardiac progenitor/stem cells (CPC/CSC) are multipotent resident populations involved in cardiac homeostasis and heart repair. Assisted by complementary RNAseq analysis, we defined the fraction of the CPC proteome associable with specific functions by comparison with human bone marrow mesenchy...

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Autores principales: Torán, José Luis, López, Juan Antonio, Gomes-Alves, Patricia, Aguilar, Susana, Torroja, Carlos, Trevisan-Herraz, Marco, Moscoso, Isabel, Sebastião, Maria João, Serra, Margarida, Brito, Catarina, Cruz, Francisco Miguel, Sepúlveda, Juan Carlos, Abad, José Luis, Galán-Arriola, Carlos, Ibanez, Borja, Martínez, Fernando, Fernández, María Eugenia, Fernández-Aviles, Francisco, Palacios, Itziar, R-Borlado, Luis, Vázquez, Jesús, Alves, Paula M., Bernad, Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6420620/
https://www.ncbi.nlm.nih.gov/pubmed/30874584
http://dx.doi.org/10.1038/s41598-019-39571-x
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author Torán, José Luis
López, Juan Antonio
Gomes-Alves, Patricia
Aguilar, Susana
Torroja, Carlos
Trevisan-Herraz, Marco
Moscoso, Isabel
Sebastião, Maria João
Serra, Margarida
Brito, Catarina
Cruz, Francisco Miguel
Sepúlveda, Juan Carlos
Abad, José Luis
Galán-Arriola, Carlos
Ibanez, Borja
Martínez, Fernando
Fernández, María Eugenia
Fernández-Aviles, Francisco
Palacios, Itziar
R-Borlado, Luis
Vázquez, Jesús
Alves, Paula M.
Bernad, Antonio
author_facet Torán, José Luis
López, Juan Antonio
Gomes-Alves, Patricia
Aguilar, Susana
Torroja, Carlos
Trevisan-Herraz, Marco
Moscoso, Isabel
Sebastião, Maria João
Serra, Margarida
Brito, Catarina
Cruz, Francisco Miguel
Sepúlveda, Juan Carlos
Abad, José Luis
Galán-Arriola, Carlos
Ibanez, Borja
Martínez, Fernando
Fernández, María Eugenia
Fernández-Aviles, Francisco
Palacios, Itziar
R-Borlado, Luis
Vázquez, Jesús
Alves, Paula M.
Bernad, Antonio
author_sort Torán, José Luis
collection PubMed
description Adult cardiac progenitor/stem cells (CPC/CSC) are multipotent resident populations involved in cardiac homeostasis and heart repair. Assisted by complementary RNAseq analysis, we defined the fraction of the CPC proteome associable with specific functions by comparison with human bone marrow mesenchymal stem cells (MSC), the reference population for cell therapy, and human dermal fibroblasts (HDF), as a distant reference. Label-free proteomic analysis identified 526 proteins expressed differentially in CPC. iTRAQ analysis confirmed differential expression of a substantial proportion of those proteins in CPC relative to MSC, and systems biology analysis defined a clear overrepresentation of several categories related to enhanced angiogenic potential. The CPC plasma membrane compartment comprised 1,595 proteins, including a minimal signature of 167 proteins preferentially or exclusively expressed by CPC. CDH5 (VE-cadherin),  OX2G (OX-2 membrane glycoprotein; CD200), GPR4 (G protein-coupled receptor 4), CACNG7 (calcium voltage-gated channel auxiliary subunit gamma 7) and F11R (F11 receptor; junctional adhesion molecule A; JAM-A; CD321) were selected for validation. Their differential expression was confirmed both in expanded CPC batches and in early stages of isolation, particularly when compared against cardiac fibroblasts. Among them, GPR4 demonstrated the highest discrimination capacity between all cell lineages analyzed.
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spelling pubmed-64206202019-03-19 Definition of a cell surface signature for human cardiac progenitor cells after comprehensive comparative transcriptomic and proteomic characterization Torán, José Luis López, Juan Antonio Gomes-Alves, Patricia Aguilar, Susana Torroja, Carlos Trevisan-Herraz, Marco Moscoso, Isabel Sebastião, Maria João Serra, Margarida Brito, Catarina Cruz, Francisco Miguel Sepúlveda, Juan Carlos Abad, José Luis Galán-Arriola, Carlos Ibanez, Borja Martínez, Fernando Fernández, María Eugenia Fernández-Aviles, Francisco Palacios, Itziar R-Borlado, Luis Vázquez, Jesús Alves, Paula M. Bernad, Antonio Sci Rep Article Adult cardiac progenitor/stem cells (CPC/CSC) are multipotent resident populations involved in cardiac homeostasis and heart repair. Assisted by complementary RNAseq analysis, we defined the fraction of the CPC proteome associable with specific functions by comparison with human bone marrow mesenchymal stem cells (MSC), the reference population for cell therapy, and human dermal fibroblasts (HDF), as a distant reference. Label-free proteomic analysis identified 526 proteins expressed differentially in CPC. iTRAQ analysis confirmed differential expression of a substantial proportion of those proteins in CPC relative to MSC, and systems biology analysis defined a clear overrepresentation of several categories related to enhanced angiogenic potential. The CPC plasma membrane compartment comprised 1,595 proteins, including a minimal signature of 167 proteins preferentially or exclusively expressed by CPC. CDH5 (VE-cadherin),  OX2G (OX-2 membrane glycoprotein; CD200), GPR4 (G protein-coupled receptor 4), CACNG7 (calcium voltage-gated channel auxiliary subunit gamma 7) and F11R (F11 receptor; junctional adhesion molecule A; JAM-A; CD321) were selected for validation. Their differential expression was confirmed both in expanded CPC batches and in early stages of isolation, particularly when compared against cardiac fibroblasts. Among them, GPR4 demonstrated the highest discrimination capacity between all cell lineages analyzed. Nature Publishing Group UK 2019-03-15 /pmc/articles/PMC6420620/ /pubmed/30874584 http://dx.doi.org/10.1038/s41598-019-39571-x Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Torán, José Luis
López, Juan Antonio
Gomes-Alves, Patricia
Aguilar, Susana
Torroja, Carlos
Trevisan-Herraz, Marco
Moscoso, Isabel
Sebastião, Maria João
Serra, Margarida
Brito, Catarina
Cruz, Francisco Miguel
Sepúlveda, Juan Carlos
Abad, José Luis
Galán-Arriola, Carlos
Ibanez, Borja
Martínez, Fernando
Fernández, María Eugenia
Fernández-Aviles, Francisco
Palacios, Itziar
R-Borlado, Luis
Vázquez, Jesús
Alves, Paula M.
Bernad, Antonio
Definition of a cell surface signature for human cardiac progenitor cells after comprehensive comparative transcriptomic and proteomic characterization
title Definition of a cell surface signature for human cardiac progenitor cells after comprehensive comparative transcriptomic and proteomic characterization
title_full Definition of a cell surface signature for human cardiac progenitor cells after comprehensive comparative transcriptomic and proteomic characterization
title_fullStr Definition of a cell surface signature for human cardiac progenitor cells after comprehensive comparative transcriptomic and proteomic characterization
title_full_unstemmed Definition of a cell surface signature for human cardiac progenitor cells after comprehensive comparative transcriptomic and proteomic characterization
title_short Definition of a cell surface signature for human cardiac progenitor cells after comprehensive comparative transcriptomic and proteomic characterization
title_sort definition of a cell surface signature for human cardiac progenitor cells after comprehensive comparative transcriptomic and proteomic characterization
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6420620/
https://www.ncbi.nlm.nih.gov/pubmed/30874584
http://dx.doi.org/10.1038/s41598-019-39571-x
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