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Absence of the proteoglycan decorin reduces glucose tolerance in overfed male mice

Studies have implicated the extracellular matrix (ECM) of adipose tissue in insulin resistance. The proteoglycan decorin, a component of ECM, has been associated with glucose tolerance, but possible causal effects on metabolism remain to be explored. We here sought to determine metabolic consequence...

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Autores principales: Svärd, Jessica, Røst, Therese H., Sommervoll, Camilla E. N., Haugen, Christine, Gudbrandsen, Oddrun A., Mellgren, Anne E., Rødahl, Eyvind, Fernø, Johan, Dankel, Simon N., Sagen, Jørn V., Mellgren, Gunnar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6420637/
https://www.ncbi.nlm.nih.gov/pubmed/30874564
http://dx.doi.org/10.1038/s41598-018-37501-x
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author Svärd, Jessica
Røst, Therese H.
Sommervoll, Camilla E. N.
Haugen, Christine
Gudbrandsen, Oddrun A.
Mellgren, Anne E.
Rødahl, Eyvind
Fernø, Johan
Dankel, Simon N.
Sagen, Jørn V.
Mellgren, Gunnar
author_facet Svärd, Jessica
Røst, Therese H.
Sommervoll, Camilla E. N.
Haugen, Christine
Gudbrandsen, Oddrun A.
Mellgren, Anne E.
Rødahl, Eyvind
Fernø, Johan
Dankel, Simon N.
Sagen, Jørn V.
Mellgren, Gunnar
author_sort Svärd, Jessica
collection PubMed
description Studies have implicated the extracellular matrix (ECM) of adipose tissue in insulin resistance. The proteoglycan decorin, a component of ECM, has been associated with glucose tolerance, but possible causal effects on metabolism remain to be explored. We here sought to determine metabolic consequences of loss of decorin in mice (DcnKO). DcnKO mice were fed a low-fat (LF) or high-fat (HF) diet for 10 weeks and body weight and food intake was recorded. An intraperitoneal glucose tolerance test was performed after eight weeks. Blood samples and adipose, liver and muscle tissues were collected at sacrifice. Global gene expression was measured in adipose tissue, and expression of decorin was also analyzed in human adipose samples. DcnKO mice showed increased feed efficiency during overfeeding and impaired glucose tolerance. Adipose leptin mRNA and circulating leptin levels were elevated in DcnKO mice, along with a downregulation of genes involved in ECM organization and triglyceride biosynthesis, and an upregulation of adipose genes involved in complement and coagulation cascades. Consistent with a protective metabolic role for decorin, in obese patients we found increased adipose decorin expression after profound fat loss, particularly in the stromal vascular fraction. Loss of decorin in mice caused impaired glucose tolerance in association with increased feed efficiency and altered gene expression in adipose tissue. Our data provide evidence that decorin is an important factor for maintaining glucose tolerance.
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spelling pubmed-64206372019-03-19 Absence of the proteoglycan decorin reduces glucose tolerance in overfed male mice Svärd, Jessica Røst, Therese H. Sommervoll, Camilla E. N. Haugen, Christine Gudbrandsen, Oddrun A. Mellgren, Anne E. Rødahl, Eyvind Fernø, Johan Dankel, Simon N. Sagen, Jørn V. Mellgren, Gunnar Sci Rep Article Studies have implicated the extracellular matrix (ECM) of adipose tissue in insulin resistance. The proteoglycan decorin, a component of ECM, has been associated with glucose tolerance, but possible causal effects on metabolism remain to be explored. We here sought to determine metabolic consequences of loss of decorin in mice (DcnKO). DcnKO mice were fed a low-fat (LF) or high-fat (HF) diet for 10 weeks and body weight and food intake was recorded. An intraperitoneal glucose tolerance test was performed after eight weeks. Blood samples and adipose, liver and muscle tissues were collected at sacrifice. Global gene expression was measured in adipose tissue, and expression of decorin was also analyzed in human adipose samples. DcnKO mice showed increased feed efficiency during overfeeding and impaired glucose tolerance. Adipose leptin mRNA and circulating leptin levels were elevated in DcnKO mice, along with a downregulation of genes involved in ECM organization and triglyceride biosynthesis, and an upregulation of adipose genes involved in complement and coagulation cascades. Consistent with a protective metabolic role for decorin, in obese patients we found increased adipose decorin expression after profound fat loss, particularly in the stromal vascular fraction. Loss of decorin in mice caused impaired glucose tolerance in association with increased feed efficiency and altered gene expression in adipose tissue. Our data provide evidence that decorin is an important factor for maintaining glucose tolerance. Nature Publishing Group UK 2019-03-15 /pmc/articles/PMC6420637/ /pubmed/30874564 http://dx.doi.org/10.1038/s41598-018-37501-x Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Svärd, Jessica
Røst, Therese H.
Sommervoll, Camilla E. N.
Haugen, Christine
Gudbrandsen, Oddrun A.
Mellgren, Anne E.
Rødahl, Eyvind
Fernø, Johan
Dankel, Simon N.
Sagen, Jørn V.
Mellgren, Gunnar
Absence of the proteoglycan decorin reduces glucose tolerance in overfed male mice
title Absence of the proteoglycan decorin reduces glucose tolerance in overfed male mice
title_full Absence of the proteoglycan decorin reduces glucose tolerance in overfed male mice
title_fullStr Absence of the proteoglycan decorin reduces glucose tolerance in overfed male mice
title_full_unstemmed Absence of the proteoglycan decorin reduces glucose tolerance in overfed male mice
title_short Absence of the proteoglycan decorin reduces glucose tolerance in overfed male mice
title_sort absence of the proteoglycan decorin reduces glucose tolerance in overfed male mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6420637/
https://www.ncbi.nlm.nih.gov/pubmed/30874564
http://dx.doi.org/10.1038/s41598-018-37501-x
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