Cargando…

Identification and Phylogenetic Analysis of the Complete Chloroplast Genomes of Three Ephedra Herbs Containing Ephedrine

Ephedrae Herba and Ephedrae Radix et Rhizoma (Mahuang) have been used as Chinese herbal medicines. Ephedra plants mainly live in deserts and have good governance of desertification. Despite their important medicinal and environmental protection value, dietary supplements containing ephedrine from Ep...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Xinlian, Cui, Yingxian, Nie, Liping, Hu, Haoyu, Xu, Zhichao, Sun, Wei, Gao, Ting, Song, Jingyuan, Yao, Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6420972/
https://www.ncbi.nlm.nih.gov/pubmed/30941367
http://dx.doi.org/10.1155/2019/5921725
_version_ 1783404167585333248
author Chen, Xinlian
Cui, Yingxian
Nie, Liping
Hu, Haoyu
Xu, Zhichao
Sun, Wei
Gao, Ting
Song, Jingyuan
Yao, Hui
author_facet Chen, Xinlian
Cui, Yingxian
Nie, Liping
Hu, Haoyu
Xu, Zhichao
Sun, Wei
Gao, Ting
Song, Jingyuan
Yao, Hui
author_sort Chen, Xinlian
collection PubMed
description Ephedrae Herba and Ephedrae Radix et Rhizoma (Mahuang) have been used as Chinese herbal medicines. Ephedra plants mainly live in deserts and have good governance of desertification. Despite their important medicinal and environmental protection value, dietary supplements containing ephedrine from Ephedra species may threaten the health of people. Morphological resemblance amongst species causes difficulty in identifying the original species of Ephedra herbs. Chloroplast (CP) genome shows good prospects in identification and phylogenetic analysis. This study introduced the structures of the CP genomes of three Ephedra species and analysed their phylogenetic relationships. Three complete CP genomes of Ephedra showed four-part annular structures, namely, two single-copy regions and two inverted repeat regions. The entire CP genomes of three Ephedra species in terms of size were 109,550 bp (E. sinica), 109,667 bp (E. intermedia), and 109,558 bp (E. equisetina). Each CP genome of the three Ephedra species encoded 118 genes, including 73 protein-coding genes, 37 tRNA genes and 8 ribosomal RNA genes. Eleven high-variation regions were screened through mVISTA to be potential specific DNA barcodes for identifying Ephedra species. Maximum likelihood and maximum parsimony trees showed that CP genomes could be used to identify Ephedra species. The Ephedra species had a close phylogenetic relationship with Gnetum species and Welwitschia mirabilis. This research provided valuable information for the identification and phylogenetic analysis of gymnosperms and drug safety of Ephedra.
format Online
Article
Text
id pubmed-6420972
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-64209722019-04-02 Identification and Phylogenetic Analysis of the Complete Chloroplast Genomes of Three Ephedra Herbs Containing Ephedrine Chen, Xinlian Cui, Yingxian Nie, Liping Hu, Haoyu Xu, Zhichao Sun, Wei Gao, Ting Song, Jingyuan Yao, Hui Biomed Res Int Research Article Ephedrae Herba and Ephedrae Radix et Rhizoma (Mahuang) have been used as Chinese herbal medicines. Ephedra plants mainly live in deserts and have good governance of desertification. Despite their important medicinal and environmental protection value, dietary supplements containing ephedrine from Ephedra species may threaten the health of people. Morphological resemblance amongst species causes difficulty in identifying the original species of Ephedra herbs. Chloroplast (CP) genome shows good prospects in identification and phylogenetic analysis. This study introduced the structures of the CP genomes of three Ephedra species and analysed their phylogenetic relationships. Three complete CP genomes of Ephedra showed four-part annular structures, namely, two single-copy regions and two inverted repeat regions. The entire CP genomes of three Ephedra species in terms of size were 109,550 bp (E. sinica), 109,667 bp (E. intermedia), and 109,558 bp (E. equisetina). Each CP genome of the three Ephedra species encoded 118 genes, including 73 protein-coding genes, 37 tRNA genes and 8 ribosomal RNA genes. Eleven high-variation regions were screened through mVISTA to be potential specific DNA barcodes for identifying Ephedra species. Maximum likelihood and maximum parsimony trees showed that CP genomes could be used to identify Ephedra species. The Ephedra species had a close phylogenetic relationship with Gnetum species and Welwitschia mirabilis. This research provided valuable information for the identification and phylogenetic analysis of gymnosperms and drug safety of Ephedra. Hindawi 2019-03-03 /pmc/articles/PMC6420972/ /pubmed/30941367 http://dx.doi.org/10.1155/2019/5921725 Text en Copyright © 2019 Xinlian Chen et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Chen, Xinlian
Cui, Yingxian
Nie, Liping
Hu, Haoyu
Xu, Zhichao
Sun, Wei
Gao, Ting
Song, Jingyuan
Yao, Hui
Identification and Phylogenetic Analysis of the Complete Chloroplast Genomes of Three Ephedra Herbs Containing Ephedrine
title Identification and Phylogenetic Analysis of the Complete Chloroplast Genomes of Three Ephedra Herbs Containing Ephedrine
title_full Identification and Phylogenetic Analysis of the Complete Chloroplast Genomes of Three Ephedra Herbs Containing Ephedrine
title_fullStr Identification and Phylogenetic Analysis of the Complete Chloroplast Genomes of Three Ephedra Herbs Containing Ephedrine
title_full_unstemmed Identification and Phylogenetic Analysis of the Complete Chloroplast Genomes of Three Ephedra Herbs Containing Ephedrine
title_short Identification and Phylogenetic Analysis of the Complete Chloroplast Genomes of Three Ephedra Herbs Containing Ephedrine
title_sort identification and phylogenetic analysis of the complete chloroplast genomes of three ephedra herbs containing ephedrine
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6420972/
https://www.ncbi.nlm.nih.gov/pubmed/30941367
http://dx.doi.org/10.1155/2019/5921725
work_keys_str_mv AT chenxinlian identificationandphylogeneticanalysisofthecompletechloroplastgenomesofthreeephedraherbscontainingephedrine
AT cuiyingxian identificationandphylogeneticanalysisofthecompletechloroplastgenomesofthreeephedraherbscontainingephedrine
AT nieliping identificationandphylogeneticanalysisofthecompletechloroplastgenomesofthreeephedraherbscontainingephedrine
AT huhaoyu identificationandphylogeneticanalysisofthecompletechloroplastgenomesofthreeephedraherbscontainingephedrine
AT xuzhichao identificationandphylogeneticanalysisofthecompletechloroplastgenomesofthreeephedraherbscontainingephedrine
AT sunwei identificationandphylogeneticanalysisofthecompletechloroplastgenomesofthreeephedraherbscontainingephedrine
AT gaoting identificationandphylogeneticanalysisofthecompletechloroplastgenomesofthreeephedraherbscontainingephedrine
AT songjingyuan identificationandphylogeneticanalysisofthecompletechloroplastgenomesofthreeephedraherbscontainingephedrine
AT yaohui identificationandphylogeneticanalysisofthecompletechloroplastgenomesofthreeephedraherbscontainingephedrine