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Hepatic and Renal Toxicity Induced by TiO(2) Nanoparticles in Rats: A Morphological and Metabonomic Study
Titanium dioxide (TiO(2)) nanoparticles (NPs) are produced abundantly and are frequently used as a white pigment in the manufacture of paints, foods, paper, and toothpaste. Despite the wide ranges of uses, there is a lack of information on the impact of NPs on animal and human health. In the present...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6421043/ https://www.ncbi.nlm.nih.gov/pubmed/30941172 http://dx.doi.org/10.1155/2019/5767012 |
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author | Valentini, Xavier Rugira, Pascaline Frau, Annica Tagliatti, Vanessa Conotte, Raphaël Laurent, Sophie Colet, Jean-Marie Nonclercq, Denis |
author_facet | Valentini, Xavier Rugira, Pascaline Frau, Annica Tagliatti, Vanessa Conotte, Raphaël Laurent, Sophie Colet, Jean-Marie Nonclercq, Denis |
author_sort | Valentini, Xavier |
collection | PubMed |
description | Titanium dioxide (TiO(2)) nanoparticles (NPs) are produced abundantly and are frequently used as a white pigment in the manufacture of paints, foods, paper, and toothpaste. Despite the wide ranges of uses, there is a lack of information on the impact of NPs on animal and human health. In the present study, rats were exposed to different doses of TiO(2) nanoparticles and sacrificed, respectively, 4 days, 1 month, and 2 months after treatment. Dosage of TiO(2) in tissues was performed by ICP-AES and revealed an important accumulation of TiO(2) in the liver. The nanoparticles induced morphological and physiological alterations in liver and kidney. In the liver, these alterations mainly affect the hepatocytes located around the centrilobular veins. These cells were the site of an oxidative stress evidenced by immunocytochemical detection of 4-hydroxynonenal (4-HNE). Kupffer cells are also the site of an important oxidative stress following the massive internalization of TiO(2) nanoparticles. Enzymatic markers of liver and kidney functions (such as AST and uric acid) are also disrupted only in animals exposed to highest doses. The metabonomic approach allowed us to detect modifications in urine samples already detectable after 4 days in animals treated at the lowest dose. This metabonomic pattern testifies an oxidative stress as well as renal and hepatic alterations. |
format | Online Article Text |
id | pubmed-6421043 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-64210432019-04-02 Hepatic and Renal Toxicity Induced by TiO(2) Nanoparticles in Rats: A Morphological and Metabonomic Study Valentini, Xavier Rugira, Pascaline Frau, Annica Tagliatti, Vanessa Conotte, Raphaël Laurent, Sophie Colet, Jean-Marie Nonclercq, Denis J Toxicol Research Article Titanium dioxide (TiO(2)) nanoparticles (NPs) are produced abundantly and are frequently used as a white pigment in the manufacture of paints, foods, paper, and toothpaste. Despite the wide ranges of uses, there is a lack of information on the impact of NPs on animal and human health. In the present study, rats were exposed to different doses of TiO(2) nanoparticles and sacrificed, respectively, 4 days, 1 month, and 2 months after treatment. Dosage of TiO(2) in tissues was performed by ICP-AES and revealed an important accumulation of TiO(2) in the liver. The nanoparticles induced morphological and physiological alterations in liver and kidney. In the liver, these alterations mainly affect the hepatocytes located around the centrilobular veins. These cells were the site of an oxidative stress evidenced by immunocytochemical detection of 4-hydroxynonenal (4-HNE). Kupffer cells are also the site of an important oxidative stress following the massive internalization of TiO(2) nanoparticles. Enzymatic markers of liver and kidney functions (such as AST and uric acid) are also disrupted only in animals exposed to highest doses. The metabonomic approach allowed us to detect modifications in urine samples already detectable after 4 days in animals treated at the lowest dose. This metabonomic pattern testifies an oxidative stress as well as renal and hepatic alterations. Hindawi 2019-03-03 /pmc/articles/PMC6421043/ /pubmed/30941172 http://dx.doi.org/10.1155/2019/5767012 Text en Copyright © 2019 Xavier Valentini et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Valentini, Xavier Rugira, Pascaline Frau, Annica Tagliatti, Vanessa Conotte, Raphaël Laurent, Sophie Colet, Jean-Marie Nonclercq, Denis Hepatic and Renal Toxicity Induced by TiO(2) Nanoparticles in Rats: A Morphological and Metabonomic Study |
title | Hepatic and Renal Toxicity Induced by TiO(2) Nanoparticles in Rats: A Morphological and Metabonomic Study |
title_full | Hepatic and Renal Toxicity Induced by TiO(2) Nanoparticles in Rats: A Morphological and Metabonomic Study |
title_fullStr | Hepatic and Renal Toxicity Induced by TiO(2) Nanoparticles in Rats: A Morphological and Metabonomic Study |
title_full_unstemmed | Hepatic and Renal Toxicity Induced by TiO(2) Nanoparticles in Rats: A Morphological and Metabonomic Study |
title_short | Hepatic and Renal Toxicity Induced by TiO(2) Nanoparticles in Rats: A Morphological and Metabonomic Study |
title_sort | hepatic and renal toxicity induced by tio(2) nanoparticles in rats: a morphological and metabonomic study |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6421043/ https://www.ncbi.nlm.nih.gov/pubmed/30941172 http://dx.doi.org/10.1155/2019/5767012 |
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