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Metabonomics Study of Ginseng Glycoproteins on Improving Sleep Quality in Mice

The changes of brain metabolism in mice after injection of ginseng glycoproteins (GPr) were analyzed by gas chromatography mass spectrometry- (GC/MS-) based metabolomics platform. The relationship between sedative and hypnotic effects of ginseng glycoproteins and brain metabolism was discussed. Refe...

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Autores principales: Wang, Ying, Zhu, Difu, Chen, Yinghong, Jiang, Ruizhi, Xu, Hong, Qiu, Zhidong, Liu, Da, Luo, Haoming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6421049/
https://www.ncbi.nlm.nih.gov/pubmed/30941359
http://dx.doi.org/10.1155/2019/2561828
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author Wang, Ying
Zhu, Difu
Chen, Yinghong
Jiang, Ruizhi
Xu, Hong
Qiu, Zhidong
Liu, Da
Luo, Haoming
author_facet Wang, Ying
Zhu, Difu
Chen, Yinghong
Jiang, Ruizhi
Xu, Hong
Qiu, Zhidong
Liu, Da
Luo, Haoming
author_sort Wang, Ying
collection PubMed
description The changes of brain metabolism in mice after injection of ginseng glycoproteins (GPr) were analyzed by gas chromatography mass spectrometry- (GC/MS-) based metabolomics platform. The relationship between sedative and hypnotic effects of ginseng glycoproteins and brain metabolism was discussed. Referring to pentobarbital sodium subthreshold test, we randomly divided 20 mice into two groups: control and ginseng glycoproteins group. The mice from the control group were treated with normal saline by i.p and GPr group were treated with 60 mg/kg of GPr by i.p. The results indicated that GPr could significantly improve the sleep quality of mice. Through multivariate statistical analysis, we found that there were 23 differential metabolites in whole brain tissues between the control group and the GPr group. The pathway analysis exhibited that GPr may be involved in the regulation of the pathway including purine metabolism, nicotinate and nicotinamide metabolism, glycine, serine and threonine metabolism, arginine and proline metabolism, alanine, aspartate and glutamate metabolism, and steroid hormone biosynthesis. This work is helpful to understand the biochemical mechanism of GPr on promoting sleep and lay a foundation for further development of drugs for insomnia.
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spelling pubmed-64210492019-04-02 Metabonomics Study of Ginseng Glycoproteins on Improving Sleep Quality in Mice Wang, Ying Zhu, Difu Chen, Yinghong Jiang, Ruizhi Xu, Hong Qiu, Zhidong Liu, Da Luo, Haoming Biomed Res Int Research Article The changes of brain metabolism in mice after injection of ginseng glycoproteins (GPr) were analyzed by gas chromatography mass spectrometry- (GC/MS-) based metabolomics platform. The relationship between sedative and hypnotic effects of ginseng glycoproteins and brain metabolism was discussed. Referring to pentobarbital sodium subthreshold test, we randomly divided 20 mice into two groups: control and ginseng glycoproteins group. The mice from the control group were treated with normal saline by i.p and GPr group were treated with 60 mg/kg of GPr by i.p. The results indicated that GPr could significantly improve the sleep quality of mice. Through multivariate statistical analysis, we found that there were 23 differential metabolites in whole brain tissues between the control group and the GPr group. The pathway analysis exhibited that GPr may be involved in the regulation of the pathway including purine metabolism, nicotinate and nicotinamide metabolism, glycine, serine and threonine metabolism, arginine and proline metabolism, alanine, aspartate and glutamate metabolism, and steroid hormone biosynthesis. This work is helpful to understand the biochemical mechanism of GPr on promoting sleep and lay a foundation for further development of drugs for insomnia. Hindawi 2019-03-03 /pmc/articles/PMC6421049/ /pubmed/30941359 http://dx.doi.org/10.1155/2019/2561828 Text en Copyright © 2019 Ying Wang et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Wang, Ying
Zhu, Difu
Chen, Yinghong
Jiang, Ruizhi
Xu, Hong
Qiu, Zhidong
Liu, Da
Luo, Haoming
Metabonomics Study of Ginseng Glycoproteins on Improving Sleep Quality in Mice
title Metabonomics Study of Ginseng Glycoproteins on Improving Sleep Quality in Mice
title_full Metabonomics Study of Ginseng Glycoproteins on Improving Sleep Quality in Mice
title_fullStr Metabonomics Study of Ginseng Glycoproteins on Improving Sleep Quality in Mice
title_full_unstemmed Metabonomics Study of Ginseng Glycoproteins on Improving Sleep Quality in Mice
title_short Metabonomics Study of Ginseng Glycoproteins on Improving Sleep Quality in Mice
title_sort metabonomics study of ginseng glycoproteins on improving sleep quality in mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6421049/
https://www.ncbi.nlm.nih.gov/pubmed/30941359
http://dx.doi.org/10.1155/2019/2561828
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