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Genomic correlates of extraintestinal infection are linked with changes in cell morphology in Campylobacter jejuni

Campylobacter jejuni is the most common cause of bacterial diarrheal disease in the world. Clinical outcomes of infection can range from asymptomatic infection to life-threatening extraintestinal infections. This variability in outcomes for infected patients has raised questions as to whether geneti...

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Autores principales: Wheeler, Nicole E., Blackmore, Timothy, Reynolds, Angela D., Midwinter, Anne C., Marshall, Jonathan, French, Nigel P., Savoian, Matthew S., Gardner, Paul P., Biggs, Patrick J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6421344/
https://www.ncbi.nlm.nih.gov/pubmed/30777818
http://dx.doi.org/10.1099/mgen.0.000251
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author Wheeler, Nicole E.
Blackmore, Timothy
Reynolds, Angela D.
Midwinter, Anne C.
Marshall, Jonathan
French, Nigel P.
Savoian, Matthew S.
Gardner, Paul P.
Biggs, Patrick J.
author_facet Wheeler, Nicole E.
Blackmore, Timothy
Reynolds, Angela D.
Midwinter, Anne C.
Marshall, Jonathan
French, Nigel P.
Savoian, Matthew S.
Gardner, Paul P.
Biggs, Patrick J.
author_sort Wheeler, Nicole E.
collection PubMed
description Campylobacter jejuni is the most common cause of bacterial diarrheal disease in the world. Clinical outcomes of infection can range from asymptomatic infection to life-threatening extraintestinal infections. This variability in outcomes for infected patients has raised questions as to whether genetic differences between C. jejuni isolates contribute to their likelihood of causing severe disease. In this study, we compare the genomes of ten C. jejuni isolates that were implicated in extraintestinal infections with reference gastrointestinal isolates, in order to identify unusual patterns of sequence variation associated with infection outcome. We identified a collection of genes that display a higher burden of uncommon mutations in invasive isolates compared with gastrointestinal close relatives, including some that have been previously linked to virulence and invasiveness in C. jejuni. Among the top genes identified were mreB and pgp1, which are both involved in determining cell shape. Electron microscopy confirmed morphological differences in isolates carrying unusual sequence variants of these genes, indicating a possible relationship between extraintestinal infection and changes in cell morphology.
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spelling pubmed-64213442019-03-18 Genomic correlates of extraintestinal infection are linked with changes in cell morphology in Campylobacter jejuni Wheeler, Nicole E. Blackmore, Timothy Reynolds, Angela D. Midwinter, Anne C. Marshall, Jonathan French, Nigel P. Savoian, Matthew S. Gardner, Paul P. Biggs, Patrick J. Microb Genom Research Article Campylobacter jejuni is the most common cause of bacterial diarrheal disease in the world. Clinical outcomes of infection can range from asymptomatic infection to life-threatening extraintestinal infections. This variability in outcomes for infected patients has raised questions as to whether genetic differences between C. jejuni isolates contribute to their likelihood of causing severe disease. In this study, we compare the genomes of ten C. jejuni isolates that were implicated in extraintestinal infections with reference gastrointestinal isolates, in order to identify unusual patterns of sequence variation associated with infection outcome. We identified a collection of genes that display a higher burden of uncommon mutations in invasive isolates compared with gastrointestinal close relatives, including some that have been previously linked to virulence and invasiveness in C. jejuni. Among the top genes identified were mreB and pgp1, which are both involved in determining cell shape. Electron microscopy confirmed morphological differences in isolates carrying unusual sequence variants of these genes, indicating a possible relationship between extraintestinal infection and changes in cell morphology. Microbiology Society 2019-02-19 /pmc/articles/PMC6421344/ /pubmed/30777818 http://dx.doi.org/10.1099/mgen.0.000251 Text en © 2019 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Wheeler, Nicole E.
Blackmore, Timothy
Reynolds, Angela D.
Midwinter, Anne C.
Marshall, Jonathan
French, Nigel P.
Savoian, Matthew S.
Gardner, Paul P.
Biggs, Patrick J.
Genomic correlates of extraintestinal infection are linked with changes in cell morphology in Campylobacter jejuni
title Genomic correlates of extraintestinal infection are linked with changes in cell morphology in Campylobacter jejuni
title_full Genomic correlates of extraintestinal infection are linked with changes in cell morphology in Campylobacter jejuni
title_fullStr Genomic correlates of extraintestinal infection are linked with changes in cell morphology in Campylobacter jejuni
title_full_unstemmed Genomic correlates of extraintestinal infection are linked with changes in cell morphology in Campylobacter jejuni
title_short Genomic correlates of extraintestinal infection are linked with changes in cell morphology in Campylobacter jejuni
title_sort genomic correlates of extraintestinal infection are linked with changes in cell morphology in campylobacter jejuni
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6421344/
https://www.ncbi.nlm.nih.gov/pubmed/30777818
http://dx.doi.org/10.1099/mgen.0.000251
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