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Cyclic stretch induced oxidative stress by mitochondrial and NADPH oxidase in retinal pigment epithelial cells

BACKGROUND: Vitreomacular adhesion (VMA) has been reported to associated with age-related macular degeneration (AMD). Understanding the mechanisms underlying cyclic stretch induced in retinal pigment epithelial cells (RPE) may be important for the treatment of VMA-related AMD. METHOD: Cyclic stretch...

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Autores principales: Liang, Xida, Wang, Zengyi, Gao, Meng, Wu, Shen, Zhang, Jingxue, Liu, Qian, Yu, Yanping, Wang, Jing, Liu, Wu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6421648/
https://www.ncbi.nlm.nih.gov/pubmed/30885167
http://dx.doi.org/10.1186/s12886-019-1087-0
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author Liang, Xida
Wang, Zengyi
Gao, Meng
Wu, Shen
Zhang, Jingxue
Liu, Qian
Yu, Yanping
Wang, Jing
Liu, Wu
author_facet Liang, Xida
Wang, Zengyi
Gao, Meng
Wu, Shen
Zhang, Jingxue
Liu, Qian
Yu, Yanping
Wang, Jing
Liu, Wu
author_sort Liang, Xida
collection PubMed
description BACKGROUND: Vitreomacular adhesion (VMA) has been reported to associated with age-related macular degeneration (AMD). Understanding the mechanisms underlying cyclic stretch induced in retinal pigment epithelial cells (RPE) may be important for the treatment of VMA-related AMD. METHOD: Cyclic stretch (1HZ, 20% elongation) was applied to cultured ARPE-19 cells for 15 min, 2 h, 6 h, 12 h, 24 h by flexcell FX-5000 Tension system. Total reactive oxygen species (ROS) were detected using DCFH-DA. Mitochondrial superoxide were detected using MitoSOX Red mitochondrial superoxide indicator. NADPH oxidases (NOX) and signaling pathways, such as p38 and PKC, were detected using western blot. Apocycin (Apo) were used as NOX inhibitors. RESULT: High levels of total ROS were detected from 15 min to 24 h, whereas mitochondrial superoxide were higher only in early time. NOX2 were significantly increased at 24 h. NOX4 were significantly increased at 2 h and reach its peak at 24 h. P-p38 was significantly increased at 12 h and 24 h. P-PKC was significantly increased at 15 min and kept a persistent high level. The upregulated expression of NOX4 by cyclic stretch can be significantly decreased under p-PKC inhibitor other than p-p38 inhibitor. CONCLUSION: Cyclic stretch induce oxidative stress from both mitochodrial and NADPH oxidase in RPE cells, which may prompt oxidative damage in VMA-related AMD.
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spelling pubmed-64216482019-03-28 Cyclic stretch induced oxidative stress by mitochondrial and NADPH oxidase in retinal pigment epithelial cells Liang, Xida Wang, Zengyi Gao, Meng Wu, Shen Zhang, Jingxue Liu, Qian Yu, Yanping Wang, Jing Liu, Wu BMC Ophthalmol Research Article BACKGROUND: Vitreomacular adhesion (VMA) has been reported to associated with age-related macular degeneration (AMD). Understanding the mechanisms underlying cyclic stretch induced in retinal pigment epithelial cells (RPE) may be important for the treatment of VMA-related AMD. METHOD: Cyclic stretch (1HZ, 20% elongation) was applied to cultured ARPE-19 cells for 15 min, 2 h, 6 h, 12 h, 24 h by flexcell FX-5000 Tension system. Total reactive oxygen species (ROS) were detected using DCFH-DA. Mitochondrial superoxide were detected using MitoSOX Red mitochondrial superoxide indicator. NADPH oxidases (NOX) and signaling pathways, such as p38 and PKC, were detected using western blot. Apocycin (Apo) were used as NOX inhibitors. RESULT: High levels of total ROS were detected from 15 min to 24 h, whereas mitochondrial superoxide were higher only in early time. NOX2 were significantly increased at 24 h. NOX4 were significantly increased at 2 h and reach its peak at 24 h. P-p38 was significantly increased at 12 h and 24 h. P-PKC was significantly increased at 15 min and kept a persistent high level. The upregulated expression of NOX4 by cyclic stretch can be significantly decreased under p-PKC inhibitor other than p-p38 inhibitor. CONCLUSION: Cyclic stretch induce oxidative stress from both mitochodrial and NADPH oxidase in RPE cells, which may prompt oxidative damage in VMA-related AMD. BioMed Central 2019-03-18 /pmc/articles/PMC6421648/ /pubmed/30885167 http://dx.doi.org/10.1186/s12886-019-1087-0 Text en © The Author(s). 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Liang, Xida
Wang, Zengyi
Gao, Meng
Wu, Shen
Zhang, Jingxue
Liu, Qian
Yu, Yanping
Wang, Jing
Liu, Wu
Cyclic stretch induced oxidative stress by mitochondrial and NADPH oxidase in retinal pigment epithelial cells
title Cyclic stretch induced oxidative stress by mitochondrial and NADPH oxidase in retinal pigment epithelial cells
title_full Cyclic stretch induced oxidative stress by mitochondrial and NADPH oxidase in retinal pigment epithelial cells
title_fullStr Cyclic stretch induced oxidative stress by mitochondrial and NADPH oxidase in retinal pigment epithelial cells
title_full_unstemmed Cyclic stretch induced oxidative stress by mitochondrial and NADPH oxidase in retinal pigment epithelial cells
title_short Cyclic stretch induced oxidative stress by mitochondrial and NADPH oxidase in retinal pigment epithelial cells
title_sort cyclic stretch induced oxidative stress by mitochondrial and nadph oxidase in retinal pigment epithelial cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6421648/
https://www.ncbi.nlm.nih.gov/pubmed/30885167
http://dx.doi.org/10.1186/s12886-019-1087-0
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