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Effects of maternal inhalation of carbon black nanoparticles on reproductive and fertility parameters in a four-generation study of male mice

BACKGROUND: Previous findings indicate that in utero exposure to nanoparticles may affect the reproductive system in male offspring. Effects such as decreased sperm counts and testicular structural changes in F1 males have been reported following maternal airway exposure to carbon black during gesta...

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Autores principales: Skovmand, Astrid, Jensen, Alexander C. Ø., Maurice, Clotilde, Marchetti, Francesco, Lauvås, Anna J., Koponen, Ismo K., Jensen, Keld A., Goericke-Pesch, Sandra, Vogel, Ulla, Hougaard, Karin S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6421671/
https://www.ncbi.nlm.nih.gov/pubmed/30879468
http://dx.doi.org/10.1186/s12989-019-0295-3
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author Skovmand, Astrid
Jensen, Alexander C. Ø.
Maurice, Clotilde
Marchetti, Francesco
Lauvås, Anna J.
Koponen, Ismo K.
Jensen, Keld A.
Goericke-Pesch, Sandra
Vogel, Ulla
Hougaard, Karin S.
author_facet Skovmand, Astrid
Jensen, Alexander C. Ø.
Maurice, Clotilde
Marchetti, Francesco
Lauvås, Anna J.
Koponen, Ismo K.
Jensen, Keld A.
Goericke-Pesch, Sandra
Vogel, Ulla
Hougaard, Karin S.
author_sort Skovmand, Astrid
collection PubMed
description BACKGROUND: Previous findings indicate that in utero exposure to nanoparticles may affect the reproductive system in male offspring. Effects such as decreased sperm counts and testicular structural changes in F1 males have been reported following maternal airway exposure to carbon black during gestation. In addition, a previous study in our laboratory suggested that the effects of in utero exposure of nanoparticles may span further than the first generation, as sperm content per gram of testis was significantly lowered in F2 males. In the present study we assessed male fertility parameters following in utero inhalation exposure to carbon black in four generations of mice. RESULTS: Filter measurements demonstrated that the time-mated females were exposed to a mean total suspended particle mass concentration of 4.79 ± 1.86 or 33.87 ± 14.77 mg/m(3) for the low and high exposure, respectively. The control exposure was below the detection limit (LOD 0.08 mg/m(3)). Exposure did not affect gestation and litter parameters in any generation. No significant changes were observed in body and reproductive organ weights, epididymal sperm parameters, daily sperm production, plasma testosterone or fertility. CONCLUSION: In utero exposure to carbon black nanoparticles, at occupationally relevant exposure levels, via maternal whole body inhalation did not affect male-specific reproductive, fertility and litter parameters in four generations of mice.
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spelling pubmed-64216712019-03-28 Effects of maternal inhalation of carbon black nanoparticles on reproductive and fertility parameters in a four-generation study of male mice Skovmand, Astrid Jensen, Alexander C. Ø. Maurice, Clotilde Marchetti, Francesco Lauvås, Anna J. Koponen, Ismo K. Jensen, Keld A. Goericke-Pesch, Sandra Vogel, Ulla Hougaard, Karin S. Part Fibre Toxicol Research BACKGROUND: Previous findings indicate that in utero exposure to nanoparticles may affect the reproductive system in male offspring. Effects such as decreased sperm counts and testicular structural changes in F1 males have been reported following maternal airway exposure to carbon black during gestation. In addition, a previous study in our laboratory suggested that the effects of in utero exposure of nanoparticles may span further than the first generation, as sperm content per gram of testis was significantly lowered in F2 males. In the present study we assessed male fertility parameters following in utero inhalation exposure to carbon black in four generations of mice. RESULTS: Filter measurements demonstrated that the time-mated females were exposed to a mean total suspended particle mass concentration of 4.79 ± 1.86 or 33.87 ± 14.77 mg/m(3) for the low and high exposure, respectively. The control exposure was below the detection limit (LOD 0.08 mg/m(3)). Exposure did not affect gestation and litter parameters in any generation. No significant changes were observed in body and reproductive organ weights, epididymal sperm parameters, daily sperm production, plasma testosterone or fertility. CONCLUSION: In utero exposure to carbon black nanoparticles, at occupationally relevant exposure levels, via maternal whole body inhalation did not affect male-specific reproductive, fertility and litter parameters in four generations of mice. BioMed Central 2019-03-18 /pmc/articles/PMC6421671/ /pubmed/30879468 http://dx.doi.org/10.1186/s12989-019-0295-3 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Skovmand, Astrid
Jensen, Alexander C. Ø.
Maurice, Clotilde
Marchetti, Francesco
Lauvås, Anna J.
Koponen, Ismo K.
Jensen, Keld A.
Goericke-Pesch, Sandra
Vogel, Ulla
Hougaard, Karin S.
Effects of maternal inhalation of carbon black nanoparticles on reproductive and fertility parameters in a four-generation study of male mice
title Effects of maternal inhalation of carbon black nanoparticles on reproductive and fertility parameters in a four-generation study of male mice
title_full Effects of maternal inhalation of carbon black nanoparticles on reproductive and fertility parameters in a four-generation study of male mice
title_fullStr Effects of maternal inhalation of carbon black nanoparticles on reproductive and fertility parameters in a four-generation study of male mice
title_full_unstemmed Effects of maternal inhalation of carbon black nanoparticles on reproductive and fertility parameters in a four-generation study of male mice
title_short Effects of maternal inhalation of carbon black nanoparticles on reproductive and fertility parameters in a four-generation study of male mice
title_sort effects of maternal inhalation of carbon black nanoparticles on reproductive and fertility parameters in a four-generation study of male mice
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6421671/
https://www.ncbi.nlm.nih.gov/pubmed/30879468
http://dx.doi.org/10.1186/s12989-019-0295-3
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