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Ostkpr1 functions in anther cuticle development and pollen wall formation in rice

BACKGROUND: During pollen wall formation in flowering plants, a conserved metabolon consisting of acyl-CoA synthetase (ACOS), polyketide synthase (PKS) and tetraketide α-pyrone reductase (TKPR), is required for sporopollenin synthesis. Despite this, the precise function of each of these components i...

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Autores principales: Xu, Dawei, Qu, Shuying, Tucker, Matthew R., Zhang, Dabing, Liang, Wanqi, Shi, Jianxin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6421701/
https://www.ncbi.nlm.nih.gov/pubmed/30885140
http://dx.doi.org/10.1186/s12870-019-1711-4
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author Xu, Dawei
Qu, Shuying
Tucker, Matthew R.
Zhang, Dabing
Liang, Wanqi
Shi, Jianxin
author_facet Xu, Dawei
Qu, Shuying
Tucker, Matthew R.
Zhang, Dabing
Liang, Wanqi
Shi, Jianxin
author_sort Xu, Dawei
collection PubMed
description BACKGROUND: During pollen wall formation in flowering plants, a conserved metabolon consisting of acyl-CoA synthetase (ACOS), polyketide synthase (PKS) and tetraketide α-pyrone reductase (TKPR), is required for sporopollenin synthesis. Despite this, the precise function of each of these components in different species remains unclear. RESULTS: In this study, we characterized the function of OsTKPR1, a rice orthologue of Arabidopsis TKPR1. Loss of function of OsTKPR1 delayed tapetum degradation, reduced the levels of anther cuticular lipids, and impaired Ubisch body and pollen exine formation, resulting in complete male sterility. In addition, the phenylpropanoid pathway in mutant anthers was remarkably altered. Localization studies suggest that OsTKPR1 accumulates in the endoplasmic reticulum, while specific accumulation of OsTKPR1 mRNA in the anther tapetum and microspores is consistent with its function in anther and pollen wall development. CONCLUSIONS: Our results show that OsTKPR1 is indispensable for anther cuticle development and pollen wall formation in rice, providing new insights into the biochemical mechanisms of the conserved sporopollenin metabolon in flowering plants. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12870-019-1711-4) contains supplementary material, which is available to authorized users.
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spelling pubmed-64217012019-03-28 Ostkpr1 functions in anther cuticle development and pollen wall formation in rice Xu, Dawei Qu, Shuying Tucker, Matthew R. Zhang, Dabing Liang, Wanqi Shi, Jianxin BMC Plant Biol Research Article BACKGROUND: During pollen wall formation in flowering plants, a conserved metabolon consisting of acyl-CoA synthetase (ACOS), polyketide synthase (PKS) and tetraketide α-pyrone reductase (TKPR), is required for sporopollenin synthesis. Despite this, the precise function of each of these components in different species remains unclear. RESULTS: In this study, we characterized the function of OsTKPR1, a rice orthologue of Arabidopsis TKPR1. Loss of function of OsTKPR1 delayed tapetum degradation, reduced the levels of anther cuticular lipids, and impaired Ubisch body and pollen exine formation, resulting in complete male sterility. In addition, the phenylpropanoid pathway in mutant anthers was remarkably altered. Localization studies suggest that OsTKPR1 accumulates in the endoplasmic reticulum, while specific accumulation of OsTKPR1 mRNA in the anther tapetum and microspores is consistent with its function in anther and pollen wall development. CONCLUSIONS: Our results show that OsTKPR1 is indispensable for anther cuticle development and pollen wall formation in rice, providing new insights into the biochemical mechanisms of the conserved sporopollenin metabolon in flowering plants. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12870-019-1711-4) contains supplementary material, which is available to authorized users. BioMed Central 2019-03-18 /pmc/articles/PMC6421701/ /pubmed/30885140 http://dx.doi.org/10.1186/s12870-019-1711-4 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Xu, Dawei
Qu, Shuying
Tucker, Matthew R.
Zhang, Dabing
Liang, Wanqi
Shi, Jianxin
Ostkpr1 functions in anther cuticle development and pollen wall formation in rice
title Ostkpr1 functions in anther cuticle development and pollen wall formation in rice
title_full Ostkpr1 functions in anther cuticle development and pollen wall formation in rice
title_fullStr Ostkpr1 functions in anther cuticle development and pollen wall formation in rice
title_full_unstemmed Ostkpr1 functions in anther cuticle development and pollen wall formation in rice
title_short Ostkpr1 functions in anther cuticle development and pollen wall formation in rice
title_sort ostkpr1 functions in anther cuticle development and pollen wall formation in rice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6421701/
https://www.ncbi.nlm.nih.gov/pubmed/30885140
http://dx.doi.org/10.1186/s12870-019-1711-4
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