Cargando…

NLRP3 inflammasome, oxidative stress, and apoptosis induced in the intestine and liver of rats treated with titanium dioxide nanoparticles: in vivo and in vitro study

PURPOSE: This study evaluated the effects of titanium dioxide nanoparticles (TiO(2) NPs) on liver and intestine of normal rats. METHODS: Male rats were divided into four groups as follows: 1) control rats, 2) control rats that orally received 10 mg/kg TiO(2) NPs, 3) control rats that orally received...

Descripción completa

Detalles Bibliográficos
Autores principales: Abbasi-Oshaghi, Ebrahim, Mirzaei, Fatemeh, Pourjafar, Mona
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6421874/
https://www.ncbi.nlm.nih.gov/pubmed/30936694
http://dx.doi.org/10.2147/IJN.S192382
_version_ 1783404310801940480
author Abbasi-Oshaghi, Ebrahim
Mirzaei, Fatemeh
Pourjafar, Mona
author_facet Abbasi-Oshaghi, Ebrahim
Mirzaei, Fatemeh
Pourjafar, Mona
author_sort Abbasi-Oshaghi, Ebrahim
collection PubMed
description PURPOSE: This study evaluated the effects of titanium dioxide nanoparticles (TiO(2) NPs) on liver and intestine of normal rats. METHODS: Male rats were divided into four groups as follows: 1) control rats, 2) control rats that orally received 10 mg/kg TiO(2) NPs, 3) control rats that orally received 50 mg/kg TiO(2) NPs, and 4) control rats that orally received 100 mg/kg TiO(2) NPs. After 30 days, the NLRP3 inflammasome pathway (NLRP3, caspase-1, and IL-1β), antioxidant pathway (superoxide dismutase [SOD], glutathione peroxidase [GPx], and catalase [CAT]), inflammatory pathway (inducible nitric oxide synthase [iNOS] and tumor necrosis factor-α [TNF-α]), and the apoptosis pathway (p53, Bax, Bcl-2, and caspase-3) were determined in the intestine and liver of the rats. H&E and Masson’s trichrome (MT) staining as well as TUNEL assay were used to examine the liver and the intestine. Biochemical factors, cytotoxicity, ROS generation, and apoptosis rate were also determined in HepG2 and Caco-2 cells. RESULTS: TiO(2) NPs in a dose-dependent manner increased cytotoxicity, oxidative stress, and apoptosis rate in Caco-2 and HepG2 cells. The administration of TiO(2) NPs significantly reduced antioxidant enzyme activity and gene expressions (SOD, CAT, and GPx) as well as glutathione (GSH) levels and total antioxidant capacity (TAC) in a dose-dependent manner. TiO(2) NPs also induced the apoptosis pathway and inflammatory pathway gene expressions and caspase-3 activity in the intestine and liver. TUNEL assay was in agreement with gene expressions. TiO(2) NPs also led to morphological changes in the liver and intestine. CONCLUSION: TiO(2) NPs could have cytotoxic effects on the intestine and liver structure and function by inducing oxidative stress, inflammation, and apoptosis.
format Online
Article
Text
id pubmed-6421874
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Dove Medical Press
record_format MEDLINE/PubMed
spelling pubmed-64218742019-04-01 NLRP3 inflammasome, oxidative stress, and apoptosis induced in the intestine and liver of rats treated with titanium dioxide nanoparticles: in vivo and in vitro study Abbasi-Oshaghi, Ebrahim Mirzaei, Fatemeh Pourjafar, Mona Int J Nanomedicine Original Research PURPOSE: This study evaluated the effects of titanium dioxide nanoparticles (TiO(2) NPs) on liver and intestine of normal rats. METHODS: Male rats were divided into four groups as follows: 1) control rats, 2) control rats that orally received 10 mg/kg TiO(2) NPs, 3) control rats that orally received 50 mg/kg TiO(2) NPs, and 4) control rats that orally received 100 mg/kg TiO(2) NPs. After 30 days, the NLRP3 inflammasome pathway (NLRP3, caspase-1, and IL-1β), antioxidant pathway (superoxide dismutase [SOD], glutathione peroxidase [GPx], and catalase [CAT]), inflammatory pathway (inducible nitric oxide synthase [iNOS] and tumor necrosis factor-α [TNF-α]), and the apoptosis pathway (p53, Bax, Bcl-2, and caspase-3) were determined in the intestine and liver of the rats. H&E and Masson’s trichrome (MT) staining as well as TUNEL assay were used to examine the liver and the intestine. Biochemical factors, cytotoxicity, ROS generation, and apoptosis rate were also determined in HepG2 and Caco-2 cells. RESULTS: TiO(2) NPs in a dose-dependent manner increased cytotoxicity, oxidative stress, and apoptosis rate in Caco-2 and HepG2 cells. The administration of TiO(2) NPs significantly reduced antioxidant enzyme activity and gene expressions (SOD, CAT, and GPx) as well as glutathione (GSH) levels and total antioxidant capacity (TAC) in a dose-dependent manner. TiO(2) NPs also induced the apoptosis pathway and inflammatory pathway gene expressions and caspase-3 activity in the intestine and liver. TUNEL assay was in agreement with gene expressions. TiO(2) NPs also led to morphological changes in the liver and intestine. CONCLUSION: TiO(2) NPs could have cytotoxic effects on the intestine and liver structure and function by inducing oxidative stress, inflammation, and apoptosis. Dove Medical Press 2019-03-15 /pmc/articles/PMC6421874/ /pubmed/30936694 http://dx.doi.org/10.2147/IJN.S192382 Text en © 2019 Abbasi-Oshaghi et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Abbasi-Oshaghi, Ebrahim
Mirzaei, Fatemeh
Pourjafar, Mona
NLRP3 inflammasome, oxidative stress, and apoptosis induced in the intestine and liver of rats treated with titanium dioxide nanoparticles: in vivo and in vitro study
title NLRP3 inflammasome, oxidative stress, and apoptosis induced in the intestine and liver of rats treated with titanium dioxide nanoparticles: in vivo and in vitro study
title_full NLRP3 inflammasome, oxidative stress, and apoptosis induced in the intestine and liver of rats treated with titanium dioxide nanoparticles: in vivo and in vitro study
title_fullStr NLRP3 inflammasome, oxidative stress, and apoptosis induced in the intestine and liver of rats treated with titanium dioxide nanoparticles: in vivo and in vitro study
title_full_unstemmed NLRP3 inflammasome, oxidative stress, and apoptosis induced in the intestine and liver of rats treated with titanium dioxide nanoparticles: in vivo and in vitro study
title_short NLRP3 inflammasome, oxidative stress, and apoptosis induced in the intestine and liver of rats treated with titanium dioxide nanoparticles: in vivo and in vitro study
title_sort nlrp3 inflammasome, oxidative stress, and apoptosis induced in the intestine and liver of rats treated with titanium dioxide nanoparticles: in vivo and in vitro study
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6421874/
https://www.ncbi.nlm.nih.gov/pubmed/30936694
http://dx.doi.org/10.2147/IJN.S192382
work_keys_str_mv AT abbasioshaghiebrahim nlrp3inflammasomeoxidativestressandapoptosisinducedintheintestineandliverofratstreatedwithtitaniumdioxidenanoparticlesinvivoandinvitrostudy
AT mirzaeifatemeh nlrp3inflammasomeoxidativestressandapoptosisinducedintheintestineandliverofratstreatedwithtitaniumdioxidenanoparticlesinvivoandinvitrostudy
AT pourjafarmona nlrp3inflammasomeoxidativestressandapoptosisinducedintheintestineandliverofratstreatedwithtitaniumdioxidenanoparticlesinvivoandinvitrostudy