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Colonic M1 macrophage is associated with the prolongation of gastrointestinal motility and obesity in mice treated with vancomycin

Gut microbiota plays a pivotal role in not only the gastrointestinal (GI) immune system but also GI motility and metabolism. Antibiotic treatments are likely to affect the gut flora and GI immune system, subsequently disturbing GI motility and body metabolism. In the present study, we investigated a...

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Autores principales: Inoue, Yoshihito, Fukui, Hirokazu, Xu, Xin, Ran, Ying, Tomita, Toshihiko, Oshima, Tadayuki, Watari, Jiro, Miwa, Hiroto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6423659/
https://www.ncbi.nlm.nih.gov/pubmed/30720127
http://dx.doi.org/10.3892/mmr.2019.9920
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author Inoue, Yoshihito
Fukui, Hirokazu
Xu, Xin
Ran, Ying
Tomita, Toshihiko
Oshima, Tadayuki
Watari, Jiro
Miwa, Hiroto
author_facet Inoue, Yoshihito
Fukui, Hirokazu
Xu, Xin
Ran, Ying
Tomita, Toshihiko
Oshima, Tadayuki
Watari, Jiro
Miwa, Hiroto
author_sort Inoue, Yoshihito
collection PubMed
description Gut microbiota plays a pivotal role in not only the gastrointestinal (GI) immune system but also GI motility and metabolism. Antibiotic treatments are likely to affect the gut flora and GI immune system, subsequently disturbing GI motility and body metabolism. In the present study, we investigated antibiotic-induced alterations of body metabolism and GI motility in association with the macrophage profile in the colon. Specific pathogen-free (SPF) mice (ICR; 6 weeks old; female) were orally administered vancomycin (0.2 mg/ml) in drinking water for 5 weeks, and subsequent changes in pathophysiology were observed. The expression of CD80 and CD163 was examined by immunohistochemistry and the expression of cytokines in colonic tissues was evaluated by reverse transcription-quantitative polymerase chain reaction. The gastrointestinal transit time (GITT) was measured by administration of carmine red (6% w/v) solution. In the vancomycin-treated SPF mice, significant increases in body weight, cecum weight and GITT were observed compared with the controls. The number of CD80-positive M1 macrophages and the expression of interferon-γ and interleukin-12 were significantly increased, whereas, the numbers of CD163-positive M2 macrophages in the mucosal and muscular layers were decreased in the colon of vancomycin-treated mice. GITT was positively correlated with the number of CD80-positive M1 macrophages in the colonic mucosa; however, was negatively correlated with the number of CD163-positive M2 macrophages in the mucosal and muscular layers. Therefore, it was suggested that antibiotic treatment affects body metabolism and GI motility, accompanied by alterations in macrophage polarization and cytokine profiles in the colon.
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spelling pubmed-64236592019-03-22 Colonic M1 macrophage is associated with the prolongation of gastrointestinal motility and obesity in mice treated with vancomycin Inoue, Yoshihito Fukui, Hirokazu Xu, Xin Ran, Ying Tomita, Toshihiko Oshima, Tadayuki Watari, Jiro Miwa, Hiroto Mol Med Rep Articles Gut microbiota plays a pivotal role in not only the gastrointestinal (GI) immune system but also GI motility and metabolism. Antibiotic treatments are likely to affect the gut flora and GI immune system, subsequently disturbing GI motility and body metabolism. In the present study, we investigated antibiotic-induced alterations of body metabolism and GI motility in association with the macrophage profile in the colon. Specific pathogen-free (SPF) mice (ICR; 6 weeks old; female) were orally administered vancomycin (0.2 mg/ml) in drinking water for 5 weeks, and subsequent changes in pathophysiology were observed. The expression of CD80 and CD163 was examined by immunohistochemistry and the expression of cytokines in colonic tissues was evaluated by reverse transcription-quantitative polymerase chain reaction. The gastrointestinal transit time (GITT) was measured by administration of carmine red (6% w/v) solution. In the vancomycin-treated SPF mice, significant increases in body weight, cecum weight and GITT were observed compared with the controls. The number of CD80-positive M1 macrophages and the expression of interferon-γ and interleukin-12 were significantly increased, whereas, the numbers of CD163-positive M2 macrophages in the mucosal and muscular layers were decreased in the colon of vancomycin-treated mice. GITT was positively correlated with the number of CD80-positive M1 macrophages in the colonic mucosa; however, was negatively correlated with the number of CD163-positive M2 macrophages in the mucosal and muscular layers. Therefore, it was suggested that antibiotic treatment affects body metabolism and GI motility, accompanied by alterations in macrophage polarization and cytokine profiles in the colon. D.A. Spandidos 2019-04 2019-01-31 /pmc/articles/PMC6423659/ /pubmed/30720127 http://dx.doi.org/10.3892/mmr.2019.9920 Text en Copyright: © Inoue et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Inoue, Yoshihito
Fukui, Hirokazu
Xu, Xin
Ran, Ying
Tomita, Toshihiko
Oshima, Tadayuki
Watari, Jiro
Miwa, Hiroto
Colonic M1 macrophage is associated with the prolongation of gastrointestinal motility and obesity in mice treated with vancomycin
title Colonic M1 macrophage is associated with the prolongation of gastrointestinal motility and obesity in mice treated with vancomycin
title_full Colonic M1 macrophage is associated with the prolongation of gastrointestinal motility and obesity in mice treated with vancomycin
title_fullStr Colonic M1 macrophage is associated with the prolongation of gastrointestinal motility and obesity in mice treated with vancomycin
title_full_unstemmed Colonic M1 macrophage is associated with the prolongation of gastrointestinal motility and obesity in mice treated with vancomycin
title_short Colonic M1 macrophage is associated with the prolongation of gastrointestinal motility and obesity in mice treated with vancomycin
title_sort colonic m1 macrophage is associated with the prolongation of gastrointestinal motility and obesity in mice treated with vancomycin
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6423659/
https://www.ncbi.nlm.nih.gov/pubmed/30720127
http://dx.doi.org/10.3892/mmr.2019.9920
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