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Insights into the Biology of Hearing and Deafness Revealed by Single-Cell RNA Sequencing

Single-cell RNA sequencing is a powerful tool by which to characterize the transcriptional profile of low-abundance cell types, but its application to the inner ear has been hampered by the bony labyrinth, tissue sparsity, and difficulty dissociating the ultra-rare cells of the membranous cochlea. H...

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Autores principales: Ranum, Paul T., Goodwin, Alexander T., Yoshimura, Hidekane, Kolbe, Diana L., Walls, William D., Koh, Jin-Young, He, David Z.Z., Smith, Richard J.H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6424336/
https://www.ncbi.nlm.nih.gov/pubmed/30865901
http://dx.doi.org/10.1016/j.celrep.2019.02.053
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author Ranum, Paul T.
Goodwin, Alexander T.
Yoshimura, Hidekane
Kolbe, Diana L.
Walls, William D.
Koh, Jin-Young
He, David Z.Z.
Smith, Richard J.H.
author_facet Ranum, Paul T.
Goodwin, Alexander T.
Yoshimura, Hidekane
Kolbe, Diana L.
Walls, William D.
Koh, Jin-Young
He, David Z.Z.
Smith, Richard J.H.
author_sort Ranum, Paul T.
collection PubMed
description Single-cell RNA sequencing is a powerful tool by which to characterize the transcriptional profile of low-abundance cell types, but its application to the inner ear has been hampered by the bony labyrinth, tissue sparsity, and difficulty dissociating the ultra-rare cells of the membranous cochlea. Herein, we present a method to isolate individual inner hair cells (IHCs), outer hair cells (OHCs), and Deiters’ cells (DCs) from the murine cochlea at any post-natal time point. We harvested more than 200 murine IHCs, OHCs, and DCs from post-natal days 15 (p15) to 228 (p228) and leveraged both short- and long-read single-cell RNA sequencing to profile transcript abundance and structure. Our results provide insights into the expression profiles of these cells and document an unappreciated complexity in isoform variety in deafness-associated genes. This refined view of transcription in the organ of Corti improves our understanding of the biology of hearing and deafness.
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spelling pubmed-64243362019-03-19 Insights into the Biology of Hearing and Deafness Revealed by Single-Cell RNA Sequencing Ranum, Paul T. Goodwin, Alexander T. Yoshimura, Hidekane Kolbe, Diana L. Walls, William D. Koh, Jin-Young He, David Z.Z. Smith, Richard J.H. Cell Rep Article Single-cell RNA sequencing is a powerful tool by which to characterize the transcriptional profile of low-abundance cell types, but its application to the inner ear has been hampered by the bony labyrinth, tissue sparsity, and difficulty dissociating the ultra-rare cells of the membranous cochlea. Herein, we present a method to isolate individual inner hair cells (IHCs), outer hair cells (OHCs), and Deiters’ cells (DCs) from the murine cochlea at any post-natal time point. We harvested more than 200 murine IHCs, OHCs, and DCs from post-natal days 15 (p15) to 228 (p228) and leveraged both short- and long-read single-cell RNA sequencing to profile transcript abundance and structure. Our results provide insights into the expression profiles of these cells and document an unappreciated complexity in isoform variety in deafness-associated genes. This refined view of transcription in the organ of Corti improves our understanding of the biology of hearing and deafness. 2019-03-12 /pmc/articles/PMC6424336/ /pubmed/30865901 http://dx.doi.org/10.1016/j.celrep.2019.02.053 Text en This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Ranum, Paul T.
Goodwin, Alexander T.
Yoshimura, Hidekane
Kolbe, Diana L.
Walls, William D.
Koh, Jin-Young
He, David Z.Z.
Smith, Richard J.H.
Insights into the Biology of Hearing and Deafness Revealed by Single-Cell RNA Sequencing
title Insights into the Biology of Hearing and Deafness Revealed by Single-Cell RNA Sequencing
title_full Insights into the Biology of Hearing and Deafness Revealed by Single-Cell RNA Sequencing
title_fullStr Insights into the Biology of Hearing and Deafness Revealed by Single-Cell RNA Sequencing
title_full_unstemmed Insights into the Biology of Hearing and Deafness Revealed by Single-Cell RNA Sequencing
title_short Insights into the Biology of Hearing and Deafness Revealed by Single-Cell RNA Sequencing
title_sort insights into the biology of hearing and deafness revealed by single-cell rna sequencing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6424336/
https://www.ncbi.nlm.nih.gov/pubmed/30865901
http://dx.doi.org/10.1016/j.celrep.2019.02.053
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