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The effectiveness of colchicine combined with mitomycin C to prolong bleb function in trabeculectomy in rabbits

PURPOSE: To investigate the potential of colchicine to improve bleb function after trabeculectomy. METHODS: To find the maximum usable colchicine concentration, an ocular irritation study was performed with the Draize test at concentrations of 0.001%, 0.01% and 0.1%. Additionally, the synergistic ef...

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Autores principales: Kokubun, Taiki, Yamamoto, Kotaro, Sato, Kota, Akaishi, Takahiro, Shimazaki, Atsushi, Nakamura, Masatsugu, Shiga, Yukihiro, Tsuda, Satoru, Omodaka, Kazuko, Nakazawa, Toru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6424470/
https://www.ncbi.nlm.nih.gov/pubmed/30889194
http://dx.doi.org/10.1371/journal.pone.0213811
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author Kokubun, Taiki
Yamamoto, Kotaro
Sato, Kota
Akaishi, Takahiro
Shimazaki, Atsushi
Nakamura, Masatsugu
Shiga, Yukihiro
Tsuda, Satoru
Omodaka, Kazuko
Nakazawa, Toru
author_facet Kokubun, Taiki
Yamamoto, Kotaro
Sato, Kota
Akaishi, Takahiro
Shimazaki, Atsushi
Nakamura, Masatsugu
Shiga, Yukihiro
Tsuda, Satoru
Omodaka, Kazuko
Nakazawa, Toru
author_sort Kokubun, Taiki
collection PubMed
description PURPOSE: To investigate the potential of colchicine to improve bleb function after trabeculectomy. METHODS: To find the maximum usable colchicine concentration, an ocular irritation study was performed with the Draize test at concentrations of 0.001%, 0.01% and 0.1%. Additionally, the synergistic effect of topical colchicine instillation and MMC application to surgical site was evaluated in a rabbit model by measuring changes after trabeculectomy in intraocular pressure (IOP) and bleb morphology score at 3, 7, 14, 21, 28, 35, 42, and 49 days. RESULTS: Experiments with a rabbit model of trabeculectomy showed that 0.04% MMC plus 0.01% colchicine was more effective than saline and 0.04% MMC alone in maintaining IOP reduction at days 7–49 (P < 0.01 at all time points) and day 49 (P < 0.05), respectively, while 0.04% MMC alone was more effective than saline only at days 7–35 (P < 0.05 at all time points). 0.04% MMC plus 0.01% colchicine and 0.04% MMC alone were more effective than saline at preserving bleb score at days 7–21 and 35–49 (P < 0.05 at all time points) and at days 7–35 (P < 0.05 at all time points), respectively. CONCLUSION: Colchicine may be a promising adjuvant for strengthening the effect of MMC and improving the survival of the filtering bleb in trabeculectomy.
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spelling pubmed-64244702019-04-02 The effectiveness of colchicine combined with mitomycin C to prolong bleb function in trabeculectomy in rabbits Kokubun, Taiki Yamamoto, Kotaro Sato, Kota Akaishi, Takahiro Shimazaki, Atsushi Nakamura, Masatsugu Shiga, Yukihiro Tsuda, Satoru Omodaka, Kazuko Nakazawa, Toru PLoS One Research Article PURPOSE: To investigate the potential of colchicine to improve bleb function after trabeculectomy. METHODS: To find the maximum usable colchicine concentration, an ocular irritation study was performed with the Draize test at concentrations of 0.001%, 0.01% and 0.1%. Additionally, the synergistic effect of topical colchicine instillation and MMC application to surgical site was evaluated in a rabbit model by measuring changes after trabeculectomy in intraocular pressure (IOP) and bleb morphology score at 3, 7, 14, 21, 28, 35, 42, and 49 days. RESULTS: Experiments with a rabbit model of trabeculectomy showed that 0.04% MMC plus 0.01% colchicine was more effective than saline and 0.04% MMC alone in maintaining IOP reduction at days 7–49 (P < 0.01 at all time points) and day 49 (P < 0.05), respectively, while 0.04% MMC alone was more effective than saline only at days 7–35 (P < 0.05 at all time points). 0.04% MMC plus 0.01% colchicine and 0.04% MMC alone were more effective than saline at preserving bleb score at days 7–21 and 35–49 (P < 0.05 at all time points) and at days 7–35 (P < 0.05 at all time points), respectively. CONCLUSION: Colchicine may be a promising adjuvant for strengthening the effect of MMC and improving the survival of the filtering bleb in trabeculectomy. Public Library of Science 2019-03-19 /pmc/articles/PMC6424470/ /pubmed/30889194 http://dx.doi.org/10.1371/journal.pone.0213811 Text en © 2019 Kokubun et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Kokubun, Taiki
Yamamoto, Kotaro
Sato, Kota
Akaishi, Takahiro
Shimazaki, Atsushi
Nakamura, Masatsugu
Shiga, Yukihiro
Tsuda, Satoru
Omodaka, Kazuko
Nakazawa, Toru
The effectiveness of colchicine combined with mitomycin C to prolong bleb function in trabeculectomy in rabbits
title The effectiveness of colchicine combined with mitomycin C to prolong bleb function in trabeculectomy in rabbits
title_full The effectiveness of colchicine combined with mitomycin C to prolong bleb function in trabeculectomy in rabbits
title_fullStr The effectiveness of colchicine combined with mitomycin C to prolong bleb function in trabeculectomy in rabbits
title_full_unstemmed The effectiveness of colchicine combined with mitomycin C to prolong bleb function in trabeculectomy in rabbits
title_short The effectiveness of colchicine combined with mitomycin C to prolong bleb function in trabeculectomy in rabbits
title_sort effectiveness of colchicine combined with mitomycin c to prolong bleb function in trabeculectomy in rabbits
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6424470/
https://www.ncbi.nlm.nih.gov/pubmed/30889194
http://dx.doi.org/10.1371/journal.pone.0213811
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