Circular synthesized CRISPR/Cas gRNAs for functional interrogations in the coding and noncoding genome

Current technologies used to generate CRISPR/Cas gene perturbation reagents are labor intense and require multiple ligation and cloning steps. Furthermore, increasing gRNA sequence diversity negatively affects gRNA distribution, leading to libraries of heterogeneous quality. Here, we present a rapid...

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Detalles Bibliográficos
Autores principales: Wegner, Martin, Diehl, Valentina, Bittl, Verena, de Bruyn, Rahel, Wiechmann, Svenja, Matthess, Yves, Hebel, Marie, Hayes, Michael GB, Schaubeck, Simone, Benner, Christopher, Heinz, Sven, Bremm, Anja, Dikic, Ivan, Ernst, Andreas, Kaulich, Manuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2019
Materias:
Cell Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6424562/
https://www.ncbi.nlm.nih.gov/pubmed/30838976
http://dx.doi.org/10.7554/eLife.42549

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