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Sinsky hook assisted roll preparation (SHARP): A modified technique for Descemet membrane endothelial keratoplasty donor preparation

PURPOSE: To describe a simple technique of sinsky hook assisted roll preparation (SHARP) for Descemet membrane endothelial keratoplasty (DMEK) donor preparation. METHODS: This experimental study was conducted at National Eye Bank, India with 40 optical grade human donor corneoscleral tissues found n...

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Autores principales: Maharana, Prafulla K., Sahay, Pranita, Titiyal, Jeewan S., Sharma, Namrata
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6424714/
https://www.ncbi.nlm.nih.gov/pubmed/30930660
http://dx.doi.org/10.1016/j.sjopt.2018.12.002
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author Maharana, Prafulla K.
Sahay, Pranita
Titiyal, Jeewan S.
Sharma, Namrata
author_facet Maharana, Prafulla K.
Sahay, Pranita
Titiyal, Jeewan S.
Sharma, Namrata
author_sort Maharana, Prafulla K.
collection PubMed
description PURPOSE: To describe a simple technique of sinsky hook assisted roll preparation (SHARP) for Descemet membrane endothelial keratoplasty (DMEK) donor preparation. METHODS: This experimental study was conducted at National Eye Bank, India with 40 optical grade human donor corneoscleral tissues found not suitable for surgery. 25 tissues were initially used to standardize the technique and remaining 15 for establishing the final technique. Donor corneal tissues were initially placed on a sterile Teflon block partially filled with tissue culture media. Initially, a partial thickness trephination was done followed by sinsky assisted 360° separation of the Descemet membrane (DM) from the underlying stroma (2 mm from the edge). The separation was further extended by 3–4 mm from the edge for 4–5 clock hours followed by bimanual peeling of the DM. This was followed by central 8 mm trephination. The primary outcome measures were a complete success (8 mm roll without peripheral edge tears) and partial success (8 mm roll with peripheral edge tears). RESULTS: DMEK roll was successfully peeled in 86.6% tissues (n = 13/15). Complete success was obtained in 66.6% tissues while partial success was obtained in 20% tissues. The median age of donor tissue was 45 years. The donor age of tissues, from which DMEK roll could not be obtained (2/12) was 15 days and 18 years. CONCLUSION: SHARP is a simple technique of DMEK that does not require any sophisticated instruments.
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spelling pubmed-64247142019-03-29 Sinsky hook assisted roll preparation (SHARP): A modified technique for Descemet membrane endothelial keratoplasty donor preparation Maharana, Prafulla K. Sahay, Pranita Titiyal, Jeewan S. Sharma, Namrata Saudi J Ophthalmol Original Article PURPOSE: To describe a simple technique of sinsky hook assisted roll preparation (SHARP) for Descemet membrane endothelial keratoplasty (DMEK) donor preparation. METHODS: This experimental study was conducted at National Eye Bank, India with 40 optical grade human donor corneoscleral tissues found not suitable for surgery. 25 tissues were initially used to standardize the technique and remaining 15 for establishing the final technique. Donor corneal tissues were initially placed on a sterile Teflon block partially filled with tissue culture media. Initially, a partial thickness trephination was done followed by sinsky assisted 360° separation of the Descemet membrane (DM) from the underlying stroma (2 mm from the edge). The separation was further extended by 3–4 mm from the edge for 4–5 clock hours followed by bimanual peeling of the DM. This was followed by central 8 mm trephination. The primary outcome measures were a complete success (8 mm roll without peripheral edge tears) and partial success (8 mm roll with peripheral edge tears). RESULTS: DMEK roll was successfully peeled in 86.6% tissues (n = 13/15). Complete success was obtained in 66.6% tissues while partial success was obtained in 20% tissues. The median age of donor tissue was 45 years. The donor age of tissues, from which DMEK roll could not be obtained (2/12) was 15 days and 18 years. CONCLUSION: SHARP is a simple technique of DMEK that does not require any sophisticated instruments. Elsevier 2019 2018-12-08 /pmc/articles/PMC6424714/ /pubmed/30930660 http://dx.doi.org/10.1016/j.sjopt.2018.12.002 Text en © 2018 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Maharana, Prafulla K.
Sahay, Pranita
Titiyal, Jeewan S.
Sharma, Namrata
Sinsky hook assisted roll preparation (SHARP): A modified technique for Descemet membrane endothelial keratoplasty donor preparation
title Sinsky hook assisted roll preparation (SHARP): A modified technique for Descemet membrane endothelial keratoplasty donor preparation
title_full Sinsky hook assisted roll preparation (SHARP): A modified technique for Descemet membrane endothelial keratoplasty donor preparation
title_fullStr Sinsky hook assisted roll preparation (SHARP): A modified technique for Descemet membrane endothelial keratoplasty donor preparation
title_full_unstemmed Sinsky hook assisted roll preparation (SHARP): A modified technique for Descemet membrane endothelial keratoplasty donor preparation
title_short Sinsky hook assisted roll preparation (SHARP): A modified technique for Descemet membrane endothelial keratoplasty donor preparation
title_sort sinsky hook assisted roll preparation (sharp): a modified technique for descemet membrane endothelial keratoplasty donor preparation
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6424714/
https://www.ncbi.nlm.nih.gov/pubmed/30930660
http://dx.doi.org/10.1016/j.sjopt.2018.12.002
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