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Measuring the impact of olive pomace enriched biscuits on the gut microbiota and its metabolic activity in mildly hypercholesterolaemic subjects

PURPOSE: Olive pomace is a major waste product of olive oil production but remains rich in polyphenols and fibres. We measured the potential of an olive pomace-enriched biscuit formulation delivering 17.1 ± 4.01 mg/100 g of hydroxytyrosol and its derivatives, to modulate the composition and metaboli...

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Autores principales: Conterno, Lorenza, Martinelli, Francesca, Tamburini, Matteo, Fava, Francesca, Mancini, Andrea, Sordo, Maddalena, Pindo, Massimo, Martens, Stefan, Masuero, Domenico, Vrhovsek, Urska, Dal Lago, Claudia, Ferrario, Gabriele, Morandini, Mario, Tuohy, Kieran
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6424929/
https://www.ncbi.nlm.nih.gov/pubmed/29124388
http://dx.doi.org/10.1007/s00394-017-1572-2
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author Conterno, Lorenza
Martinelli, Francesca
Tamburini, Matteo
Fava, Francesca
Mancini, Andrea
Sordo, Maddalena
Pindo, Massimo
Martens, Stefan
Masuero, Domenico
Vrhovsek, Urska
Dal Lago, Claudia
Ferrario, Gabriele
Morandini, Mario
Tuohy, Kieran
author_facet Conterno, Lorenza
Martinelli, Francesca
Tamburini, Matteo
Fava, Francesca
Mancini, Andrea
Sordo, Maddalena
Pindo, Massimo
Martens, Stefan
Masuero, Domenico
Vrhovsek, Urska
Dal Lago, Claudia
Ferrario, Gabriele
Morandini, Mario
Tuohy, Kieran
author_sort Conterno, Lorenza
collection PubMed
description PURPOSE: Olive pomace is a major waste product of olive oil production but remains rich in polyphenols and fibres. We measured the potential of an olive pomace-enriched biscuit formulation delivering 17.1 ± 4.01 mg/100 g of hydroxytyrosol and its derivatives, to modulate the composition and metabolic activity of the human gut microbiota. METHODS: In a double-blind, controlled parallel dietary intervention 62 otherwise healthy hypercholesterolemic (total plasma cholesterol 180–240 mg/dl) subjects were randomly assigned to eat 90 g of olive pomace-enriched biscuit (olive-enriched product, OEP) or an isoenergetic control (CTRL) for 8 weeks. Fasted blood samples, 24-h urine and faecal samples were collected before and after dietary intervention for measurement of microbiota, metabolites and clinical parameters. RESULTS: Consumption of OEP biscuits did not impact on the diversity of the faecal microbiota and there was no statistically significant effect on CVD markers. A trend towards reduced oxidized LDL cholesterol following OEP ingestion was observed. At the genus level lactobacilli and Ruminococcus were reduced in OEP compared to CTRL biscuits. A trend towards increased bifidobacteria abundance was observed after OEP ingestion in 16S rRNA profiles, by fluorescent in situ hybridization and by qPCR. Targeted LC–MS revealed significant increases phenolic acid concentrations in 24-h urine following OEP ingestion and 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid, derivatives of hydroxytyrosol, were elevated in blood. A sex effect was apparent in urine small phenolic acid concentrations, and this sex effect was mirrored by statistically significant differences in relative abundances of faecal bacteria between men and women. CONCLUSION: Ingestion of OEP biscuits led to a significant increase in the metabolic output of the gut microbiota with an apparent sex effect possibly linked to differences in microbiota makeup. Increased levels of homovanillic acid and DOPAC, thought to be involved in reducing oxidative LDL cholesterol, were observed upon OEP ingestion. However, OEP did not induce statistically significant changes in either ox-LDL or urinary isoprostane in this study.
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spelling pubmed-64249292019-04-05 Measuring the impact of olive pomace enriched biscuits on the gut microbiota and its metabolic activity in mildly hypercholesterolaemic subjects Conterno, Lorenza Martinelli, Francesca Tamburini, Matteo Fava, Francesca Mancini, Andrea Sordo, Maddalena Pindo, Massimo Martens, Stefan Masuero, Domenico Vrhovsek, Urska Dal Lago, Claudia Ferrario, Gabriele Morandini, Mario Tuohy, Kieran Eur J Nutr Original Contribution PURPOSE: Olive pomace is a major waste product of olive oil production but remains rich in polyphenols and fibres. We measured the potential of an olive pomace-enriched biscuit formulation delivering 17.1 ± 4.01 mg/100 g of hydroxytyrosol and its derivatives, to modulate the composition and metabolic activity of the human gut microbiota. METHODS: In a double-blind, controlled parallel dietary intervention 62 otherwise healthy hypercholesterolemic (total plasma cholesterol 180–240 mg/dl) subjects were randomly assigned to eat 90 g of olive pomace-enriched biscuit (olive-enriched product, OEP) or an isoenergetic control (CTRL) for 8 weeks. Fasted blood samples, 24-h urine and faecal samples were collected before and after dietary intervention for measurement of microbiota, metabolites and clinical parameters. RESULTS: Consumption of OEP biscuits did not impact on the diversity of the faecal microbiota and there was no statistically significant effect on CVD markers. A trend towards reduced oxidized LDL cholesterol following OEP ingestion was observed. At the genus level lactobacilli and Ruminococcus were reduced in OEP compared to CTRL biscuits. A trend towards increased bifidobacteria abundance was observed after OEP ingestion in 16S rRNA profiles, by fluorescent in situ hybridization and by qPCR. Targeted LC–MS revealed significant increases phenolic acid concentrations in 24-h urine following OEP ingestion and 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid, derivatives of hydroxytyrosol, were elevated in blood. A sex effect was apparent in urine small phenolic acid concentrations, and this sex effect was mirrored by statistically significant differences in relative abundances of faecal bacteria between men and women. CONCLUSION: Ingestion of OEP biscuits led to a significant increase in the metabolic output of the gut microbiota with an apparent sex effect possibly linked to differences in microbiota makeup. Increased levels of homovanillic acid and DOPAC, thought to be involved in reducing oxidative LDL cholesterol, were observed upon OEP ingestion. However, OEP did not induce statistically significant changes in either ox-LDL or urinary isoprostane in this study. Springer Berlin Heidelberg 2017-11-09 2019 /pmc/articles/PMC6424929/ /pubmed/29124388 http://dx.doi.org/10.1007/s00394-017-1572-2 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Contribution
Conterno, Lorenza
Martinelli, Francesca
Tamburini, Matteo
Fava, Francesca
Mancini, Andrea
Sordo, Maddalena
Pindo, Massimo
Martens, Stefan
Masuero, Domenico
Vrhovsek, Urska
Dal Lago, Claudia
Ferrario, Gabriele
Morandini, Mario
Tuohy, Kieran
Measuring the impact of olive pomace enriched biscuits on the gut microbiota and its metabolic activity in mildly hypercholesterolaemic subjects
title Measuring the impact of olive pomace enriched biscuits on the gut microbiota and its metabolic activity in mildly hypercholesterolaemic subjects
title_full Measuring the impact of olive pomace enriched biscuits on the gut microbiota and its metabolic activity in mildly hypercholesterolaemic subjects
title_fullStr Measuring the impact of olive pomace enriched biscuits on the gut microbiota and its metabolic activity in mildly hypercholesterolaemic subjects
title_full_unstemmed Measuring the impact of olive pomace enriched biscuits on the gut microbiota and its metabolic activity in mildly hypercholesterolaemic subjects
title_short Measuring the impact of olive pomace enriched biscuits on the gut microbiota and its metabolic activity in mildly hypercholesterolaemic subjects
title_sort measuring the impact of olive pomace enriched biscuits on the gut microbiota and its metabolic activity in mildly hypercholesterolaemic subjects
topic Original Contribution
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6424929/
https://www.ncbi.nlm.nih.gov/pubmed/29124388
http://dx.doi.org/10.1007/s00394-017-1572-2
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