Unusual substrate and halide versatility of phenolic halogenase PltM

Controlled halogenation of chemically versatile substrates is difficult to achieve. Here we describe a unique flavin-dependent halogenase, PltM, which is capable of utilizing a wide range of halides for installation on a diverse array of phenolic compounds, including FDA-approved drugs and natural p...

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Detalles Bibliográficos
Autores principales: Mori, Shogo, Pang, Allan H., Thamban Chandrika, Nishad, Garneau-Tsodikova, Sylvie, Tsodikov, Oleg V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6424973/
https://www.ncbi.nlm.nih.gov/pubmed/30890712
http://dx.doi.org/10.1038/s41467-019-09215-9
Descripción
Sumario:Controlled halogenation of chemically versatile substrates is difficult to achieve. Here we describe a unique flavin-dependent halogenase, PltM, which is capable of utilizing a wide range of halides for installation on a diverse array of phenolic compounds, including FDA-approved drugs and natural products, such as terbutaline, fenoterol, resveratrol, and catechin. Crystal structures of PltM in complex with phloroglucinol and FAD in different states yield insight into substrate recognition and the FAD recycling mechanism of this halogenase.

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