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A new formulation of graphene oxide/fluconazole compound as a promising agent against Candida albicans
Candida albicans (C. albicans) belongs to the opportunistic fungal pathogens, which cause a wide spectrum of infections in immune-compromised patients. Graphene oxide (GO), a biocompatibility agent, has been reported to exhibit effective antimicrobial activity. In the present study, a graphene oxide...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6424987/ https://www.ncbi.nlm.nih.gov/pubmed/30859396 http://dx.doi.org/10.1007/s40204-019-0109-6 |
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author | Asadi Shahi, Sabrieh Roudbar Mohammadi, Shahla Roudbary, Maryam Delavari, Hamid |
author_facet | Asadi Shahi, Sabrieh Roudbar Mohammadi, Shahla Roudbary, Maryam Delavari, Hamid |
author_sort | Asadi Shahi, Sabrieh |
collection | PubMed |
description | Candida albicans (C. albicans) belongs to the opportunistic fungal pathogens, which cause a wide spectrum of infections in immune-compromised patients. Graphene oxide (GO), a biocompatibility agent, has been reported to exhibit effective antimicrobial activity. In the present study, a graphene oxide/fluconazole (GO/Flu) compound was synthesized and characterized using Fourier transform infrared spectroscopy (FTIR) and Raman spectroscopy. The antifungal activity of GO/Flu was examined against fluconazole-resistant C. albicans (ATCC 10231) compared to GO and Flu using the broth microdilution method, according to CLSI protocol. DNA fragmentation was assessed through the antifungal mechanism of GO/Flu. The release of Fluin PBS medium was measured. Moreover, the cytotoxicity effect of GO/Flu on SW480 cell line was evaluated. Indeed, adhesion ability of C. albicans-treated GO/Flu against SW480 cell line was assessed. The minimum inhibitory concentration (MIC) of GO, Flu, and GO/Flu was determined at 800 µg/mL, 16 µg/mL, and 400–9 µg/mL, respectively. Notably, GO/Flu exhibited an intense antifungal activity compared to GO and Flu. In addition, GO/Flu showed much less cell toxicity against SW480 cell line than GO and Flu (P < 0.05). The release determination of Flu in PBS (pH 7.4) medium was 72.42%. GO/Flu reduced the adhesion ability of C. albicans to SW480 cell line significantly. DNA fragmentation assay indicated that GO/Flu potentially degraded the DNA of C. albicans and caused a fungicidal influence. According to the findings, GO/Flu could enhance the antifungal activity against C.albicans through DNA fragmentation with low cytotoxicity effect. |
format | Online Article Text |
id | pubmed-6424987 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-64249872019-04-05 A new formulation of graphene oxide/fluconazole compound as a promising agent against Candida albicans Asadi Shahi, Sabrieh Roudbar Mohammadi, Shahla Roudbary, Maryam Delavari, Hamid Prog Biomater Original Research Candida albicans (C. albicans) belongs to the opportunistic fungal pathogens, which cause a wide spectrum of infections in immune-compromised patients. Graphene oxide (GO), a biocompatibility agent, has been reported to exhibit effective antimicrobial activity. In the present study, a graphene oxide/fluconazole (GO/Flu) compound was synthesized and characterized using Fourier transform infrared spectroscopy (FTIR) and Raman spectroscopy. The antifungal activity of GO/Flu was examined against fluconazole-resistant C. albicans (ATCC 10231) compared to GO and Flu using the broth microdilution method, according to CLSI protocol. DNA fragmentation was assessed through the antifungal mechanism of GO/Flu. The release of Fluin PBS medium was measured. Moreover, the cytotoxicity effect of GO/Flu on SW480 cell line was evaluated. Indeed, adhesion ability of C. albicans-treated GO/Flu against SW480 cell line was assessed. The minimum inhibitory concentration (MIC) of GO, Flu, and GO/Flu was determined at 800 µg/mL, 16 µg/mL, and 400–9 µg/mL, respectively. Notably, GO/Flu exhibited an intense antifungal activity compared to GO and Flu. In addition, GO/Flu showed much less cell toxicity against SW480 cell line than GO and Flu (P < 0.05). The release determination of Flu in PBS (pH 7.4) medium was 72.42%. GO/Flu reduced the adhesion ability of C. albicans to SW480 cell line significantly. DNA fragmentation assay indicated that GO/Flu potentially degraded the DNA of C. albicans and caused a fungicidal influence. According to the findings, GO/Flu could enhance the antifungal activity against C.albicans through DNA fragmentation with low cytotoxicity effect. Springer Berlin Heidelberg 2019-03-11 /pmc/articles/PMC6424987/ /pubmed/30859396 http://dx.doi.org/10.1007/s40204-019-0109-6 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Research Asadi Shahi, Sabrieh Roudbar Mohammadi, Shahla Roudbary, Maryam Delavari, Hamid A new formulation of graphene oxide/fluconazole compound as a promising agent against Candida albicans |
title | A new formulation of graphene oxide/fluconazole compound as a promising agent against Candida albicans |
title_full | A new formulation of graphene oxide/fluconazole compound as a promising agent against Candida albicans |
title_fullStr | A new formulation of graphene oxide/fluconazole compound as a promising agent against Candida albicans |
title_full_unstemmed | A new formulation of graphene oxide/fluconazole compound as a promising agent against Candida albicans |
title_short | A new formulation of graphene oxide/fluconazole compound as a promising agent against Candida albicans |
title_sort | new formulation of graphene oxide/fluconazole compound as a promising agent against candida albicans |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6424987/ https://www.ncbi.nlm.nih.gov/pubmed/30859396 http://dx.doi.org/10.1007/s40204-019-0109-6 |
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