Biofabrication and characterization of flavonoid-loaded Ag, Au, Au–Ag bimetallic nanoparticles using seed extract of the plant Madhuca longifolia for the enhancement in wound healing bio-efficacy

ABSTRACT: The present communication warrants the presence of significant wound healing bio-efficacy of aq. alc. extract of the seed (49.78%) of the plant Madhuca longifolia. A family of seven flavonoid fractions have been ascertained in the seed aq. alc. extract of the target plant using LCMS-8030 analysis. In vivo wound healing parameters (wound area, wound closure, epithelization period, skin breaking strength and hydroxyproline content) have been examined in Swiss albino mice models. Statistically significant (p < 0.001) enhancement in the wound healing bio-efficacy has been effectively induced using flavonoid-loaded gold: (M(l)f@AuNp(s))(,) silver: (M(l)f@AgNp(s)), and Au–Ag bimetallic: (M(l)f@Au–AgNp(s)) nanoparticles. Among the biofabricated nano-biomaterials, M(l)f@AgNp(s) exhibited an exceptional enhancement in the wound healing bio-efficacy (80.33%) attaining almost to the level of reference drug Placentrex (84.02%). All the fabricated nano-biomaterials were thoroughly characterized using UV–Vis, XRD, FE-SEM, TEM, EDX, and DLS. The promising enhancement in the wound healing potential of the nano-biomaterial (M(l)f@AgNp(s)) has been explained based on the cumulative effects of biological and nanotech parameters. The bio-fabricated (M(l)f@AgNp(s)) nano-biomaterials using the plant M. longifolia have lustrous prospects for the development of complimentary herbal nanomedicine for scaling-up the wound healing bio-efficacy. GRAPHICAL ABSTRACT: [Image: see text]