Cargando…
Polymorphisms in MTHFR, MTR, RFC1 and CßS genes involved in folate metabolism and thyroid cancer: a case-control study
INTRODUCTION: Polymorphisms in genes coding enzymes involved in folate metabolism may cause alterations in this metabolic pathway and contribute to carcinogenesis, because folate is essential for DNA synthesis, methylation and repair. The objective of this study was to investigate the association of...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Termedia Publishing House
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425207/ https://www.ncbi.nlm.nih.gov/pubmed/30899306 http://dx.doi.org/10.5114/aoms.2018.73091 |
_version_ | 1783404797952524288 |
---|---|
author | Zara-Lopes, Tairine Galbiatti-Dias, Ana Lívia Silva Castanhole-Nunes, Márcia M. Urbanin Padovani-Júnior, João Armando Maniglia, José Victor Pavarino, Erika Cristina Goloni-Bertollo, Eny Maria |
author_facet | Zara-Lopes, Tairine Galbiatti-Dias, Ana Lívia Silva Castanhole-Nunes, Márcia M. Urbanin Padovani-Júnior, João Armando Maniglia, José Victor Pavarino, Erika Cristina Goloni-Bertollo, Eny Maria |
author_sort | Zara-Lopes, Tairine |
collection | PubMed |
description | INTRODUCTION: Polymorphisms in genes coding enzymes involved in folate metabolism may cause alterations in this metabolic pathway and contribute to carcinogenesis, because folate is essential for DNA synthesis, methylation and repair. The objective of this study was to investigate the association of MTHFR 677C>T (rs1801133), MTR 2756A>G (rs1805087), RFC1 80A>G (rs1051266) and CßS 844ins(68) (no rs#) polymorphisms and thyroid cancer development. The association of these polymorphisms with demographic risk factors and clinical histopathological parameters was also evaluated. MATERIAL AND METHODS: The study is a case-control analysis with a total of 462 individuals (151 patients and 311 controls). Polymerase chain reaction-restriction fragment length polymorphism technique was used for genotyping. The χ(2) and multiple logistic regression were utilized for statistical analysis. RESULTS: The polymorphisms analysis revealed an association between the MTHFR 677C>T polymorphism (OR = 2.87, 95% CI: 1.50–5.48, p < 0.01, codominant model), (OR = 1.76, 95% CI: 1.18–2.64, p < 0.01, dominant model), (OR = 2.37, 95% CI: 1.28–4.39, p < 0.01, recessive model) and thyroid cancer. RFC1 80A>G polymorphism also was associated with thyroid cancer under recessive mode of inheritance (OR = 1.55; 95% CI: 1.02–2.38; p = 0.04); however, this polymorphism showed Hardy-Weinberg disequilibrium in the control group (χ(2) = 24.71, p < 0.001). Furthermore, alcohol (OR = 1.56, 95% CI: 1.36–1.89, p < 0.01) and tobacco consumption (OR = 1.97, 95% CI: 1.28–3.04, p < 0.01) were associated with increased risk for thyroid cancer. The MTR 2756A>G polymorphism showed an association with tumor extent (OR = 2.69, 95% CI: 1.27–5.71, p < 0.01) and aggressiveness (OR = 4.51, 95% CI: 1.67–12.1, p < 0.01). CONCLUSIONS: MTHFR 677C>T is significantly associated with increased risk for thyroid cancer and MTR 2756A>G is associated with tumor extent and aggressiveness. In addition, alcohol and tobacco consumption were associated with increased risk of thyroid cancer. These results may contribute to a better prognosis for thyroid cancer. |
format | Online Article Text |
id | pubmed-6425207 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Termedia Publishing House |
record_format | MEDLINE/PubMed |
spelling | pubmed-64252072019-03-21 Polymorphisms in MTHFR, MTR, RFC1 and CßS genes involved in folate metabolism and thyroid cancer: a case-control study Zara-Lopes, Tairine Galbiatti-Dias, Ana Lívia Silva Castanhole-Nunes, Márcia M. Urbanin Padovani-Júnior, João Armando Maniglia, José Victor Pavarino, Erika Cristina Goloni-Bertollo, Eny Maria Arch Med Sci Basic Research INTRODUCTION: Polymorphisms in genes coding enzymes involved in folate metabolism may cause alterations in this metabolic pathway and contribute to carcinogenesis, because folate is essential for DNA synthesis, methylation and repair. The objective of this study was to investigate the association of MTHFR 677C>T (rs1801133), MTR 2756A>G (rs1805087), RFC1 80A>G (rs1051266) and CßS 844ins(68) (no rs#) polymorphisms and thyroid cancer development. The association of these polymorphisms with demographic risk factors and clinical histopathological parameters was also evaluated. MATERIAL AND METHODS: The study is a case-control analysis with a total of 462 individuals (151 patients and 311 controls). Polymerase chain reaction-restriction fragment length polymorphism technique was used for genotyping. The χ(2) and multiple logistic regression were utilized for statistical analysis. RESULTS: The polymorphisms analysis revealed an association between the MTHFR 677C>T polymorphism (OR = 2.87, 95% CI: 1.50–5.48, p < 0.01, codominant model), (OR = 1.76, 95% CI: 1.18–2.64, p < 0.01, dominant model), (OR = 2.37, 95% CI: 1.28–4.39, p < 0.01, recessive model) and thyroid cancer. RFC1 80A>G polymorphism also was associated with thyroid cancer under recessive mode of inheritance (OR = 1.55; 95% CI: 1.02–2.38; p = 0.04); however, this polymorphism showed Hardy-Weinberg disequilibrium in the control group (χ(2) = 24.71, p < 0.001). Furthermore, alcohol (OR = 1.56, 95% CI: 1.36–1.89, p < 0.01) and tobacco consumption (OR = 1.97, 95% CI: 1.28–3.04, p < 0.01) were associated with increased risk for thyroid cancer. The MTR 2756A>G polymorphism showed an association with tumor extent (OR = 2.69, 95% CI: 1.27–5.71, p < 0.01) and aggressiveness (OR = 4.51, 95% CI: 1.67–12.1, p < 0.01). CONCLUSIONS: MTHFR 677C>T is significantly associated with increased risk for thyroid cancer and MTR 2756A>G is associated with tumor extent and aggressiveness. In addition, alcohol and tobacco consumption were associated with increased risk of thyroid cancer. These results may contribute to a better prognosis for thyroid cancer. Termedia Publishing House 2019-02-05 2019-03 /pmc/articles/PMC6425207/ /pubmed/30899306 http://dx.doi.org/10.5114/aoms.2018.73091 Text en Copyright: © 2019 Termedia & Banach http://creativecommons.org/licenses/by-nc-sa/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) License, allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material, provided the original work is properly cited and states its license. |
spellingShingle | Basic Research Zara-Lopes, Tairine Galbiatti-Dias, Ana Lívia Silva Castanhole-Nunes, Márcia M. Urbanin Padovani-Júnior, João Armando Maniglia, José Victor Pavarino, Erika Cristina Goloni-Bertollo, Eny Maria Polymorphisms in MTHFR, MTR, RFC1 and CßS genes involved in folate metabolism and thyroid cancer: a case-control study |
title | Polymorphisms in MTHFR, MTR, RFC1 and CßS genes involved in folate metabolism and thyroid cancer: a case-control study |
title_full | Polymorphisms in MTHFR, MTR, RFC1 and CßS genes involved in folate metabolism and thyroid cancer: a case-control study |
title_fullStr | Polymorphisms in MTHFR, MTR, RFC1 and CßS genes involved in folate metabolism and thyroid cancer: a case-control study |
title_full_unstemmed | Polymorphisms in MTHFR, MTR, RFC1 and CßS genes involved in folate metabolism and thyroid cancer: a case-control study |
title_short | Polymorphisms in MTHFR, MTR, RFC1 and CßS genes involved in folate metabolism and thyroid cancer: a case-control study |
title_sort | polymorphisms in mthfr, mtr, rfc1 and cßs genes involved in folate metabolism and thyroid cancer: a case-control study |
topic | Basic Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425207/ https://www.ncbi.nlm.nih.gov/pubmed/30899306 http://dx.doi.org/10.5114/aoms.2018.73091 |
work_keys_str_mv | AT zaralopestairine polymorphismsinmthfrmtrrfc1andcßsgenesinvolvedinfolatemetabolismandthyroidcanceracasecontrolstudy AT galbiattidiasanaliviasilva polymorphismsinmthfrmtrrfc1andcßsgenesinvolvedinfolatemetabolismandthyroidcanceracasecontrolstudy AT castanholenunesmarciamurbanin polymorphismsinmthfrmtrrfc1andcßsgenesinvolvedinfolatemetabolismandthyroidcanceracasecontrolstudy AT padovanijuniorjoaoarmando polymorphismsinmthfrmtrrfc1andcßsgenesinvolvedinfolatemetabolismandthyroidcanceracasecontrolstudy AT manigliajosevictor polymorphismsinmthfrmtrrfc1andcßsgenesinvolvedinfolatemetabolismandthyroidcanceracasecontrolstudy AT pavarinoerikacristina polymorphismsinmthfrmtrrfc1andcßsgenesinvolvedinfolatemetabolismandthyroidcanceracasecontrolstudy AT golonibertolloenymaria polymorphismsinmthfrmtrrfc1andcßsgenesinvolvedinfolatemetabolismandthyroidcanceracasecontrolstudy |