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Baicalein Inhibits Orthotopic Human Non-Small Cell Lung Cancer Xenografts via Src/Id1 Pathway

Non-small cell lung cancer (NSCLC) is one of the most lethal cancers worldwide. Inhibitor of differentiation 1 (Id1) is the member mostly linked to tumorigenesis in Id family and a potential molecular target in cancer therapy. In the current study, we established an orthotopic lung cancer model by i...

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Autores principales: Zhao, Zhengxiao, Liu, Baojun, Sun, Jing, Lu, Linwei, Liu, Lumei, Qiu, Jian, Li, Qiuping, Yan, Chen, Jiang, Shan, Mohammadtursun, Nabijan, Ma, Wenjuan, Li, Mihui, Dong, Jingcheng, Gong, Weiyi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425425/
https://www.ncbi.nlm.nih.gov/pubmed/30949224
http://dx.doi.org/10.1155/2019/9806062
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author Zhao, Zhengxiao
Liu, Baojun
Sun, Jing
Lu, Linwei
Liu, Lumei
Qiu, Jian
Li, Qiuping
Yan, Chen
Jiang, Shan
Mohammadtursun, Nabijan
Ma, Wenjuan
Li, Mihui
Dong, Jingcheng
Gong, Weiyi
author_facet Zhao, Zhengxiao
Liu, Baojun
Sun, Jing
Lu, Linwei
Liu, Lumei
Qiu, Jian
Li, Qiuping
Yan, Chen
Jiang, Shan
Mohammadtursun, Nabijan
Ma, Wenjuan
Li, Mihui
Dong, Jingcheng
Gong, Weiyi
author_sort Zhao, Zhengxiao
collection PubMed
description Non-small cell lung cancer (NSCLC) is one of the most lethal cancers worldwide. Inhibitor of differentiation 1 (Id1) is the member mostly linked to tumorigenesis in Id family and a potential molecular target in cancer therapy. In the current study, we established an orthotopic lung cancer model by injecting athymic nude mice with A549 cells and evaluated the antitumor effect of baicalein and expression of Id1-related proteins in vivo and in vitro. Micro-CT images showed that tumor volume in baicalein group was significantly reduced. Western blot analysis revealed that baicalein suppressed the expression of Id1 protein, epithelial-to-mesenchymal transition (EMT) related molecules (N-Cadherin, vimentin), and angiogenesis related protein (VEGF-A), accompanied by upregulation of epithelial markers (such as E-cadherin). In addition, phosphorylation of upstream molecular Src was significantly restrained after baicalein treatment. This study firstly demonstrates that baicalein inhibits tumor growth in orthotopic human NSCLC xenografts via targeting Src/Id1 pathway.
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spelling pubmed-64254252019-04-04 Baicalein Inhibits Orthotopic Human Non-Small Cell Lung Cancer Xenografts via Src/Id1 Pathway Zhao, Zhengxiao Liu, Baojun Sun, Jing Lu, Linwei Liu, Lumei Qiu, Jian Li, Qiuping Yan, Chen Jiang, Shan Mohammadtursun, Nabijan Ma, Wenjuan Li, Mihui Dong, Jingcheng Gong, Weiyi Evid Based Complement Alternat Med Research Article Non-small cell lung cancer (NSCLC) is one of the most lethal cancers worldwide. Inhibitor of differentiation 1 (Id1) is the member mostly linked to tumorigenesis in Id family and a potential molecular target in cancer therapy. In the current study, we established an orthotopic lung cancer model by injecting athymic nude mice with A549 cells and evaluated the antitumor effect of baicalein and expression of Id1-related proteins in vivo and in vitro. Micro-CT images showed that tumor volume in baicalein group was significantly reduced. Western blot analysis revealed that baicalein suppressed the expression of Id1 protein, epithelial-to-mesenchymal transition (EMT) related molecules (N-Cadherin, vimentin), and angiogenesis related protein (VEGF-A), accompanied by upregulation of epithelial markers (such as E-cadherin). In addition, phosphorylation of upstream molecular Src was significantly restrained after baicalein treatment. This study firstly demonstrates that baicalein inhibits tumor growth in orthotopic human NSCLC xenografts via targeting Src/Id1 pathway. Hindawi 2019-03-04 /pmc/articles/PMC6425425/ /pubmed/30949224 http://dx.doi.org/10.1155/2019/9806062 Text en Copyright © 2019 Zhengxiao Zhao et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhao, Zhengxiao
Liu, Baojun
Sun, Jing
Lu, Linwei
Liu, Lumei
Qiu, Jian
Li, Qiuping
Yan, Chen
Jiang, Shan
Mohammadtursun, Nabijan
Ma, Wenjuan
Li, Mihui
Dong, Jingcheng
Gong, Weiyi
Baicalein Inhibits Orthotopic Human Non-Small Cell Lung Cancer Xenografts via Src/Id1 Pathway
title Baicalein Inhibits Orthotopic Human Non-Small Cell Lung Cancer Xenografts via Src/Id1 Pathway
title_full Baicalein Inhibits Orthotopic Human Non-Small Cell Lung Cancer Xenografts via Src/Id1 Pathway
title_fullStr Baicalein Inhibits Orthotopic Human Non-Small Cell Lung Cancer Xenografts via Src/Id1 Pathway
title_full_unstemmed Baicalein Inhibits Orthotopic Human Non-Small Cell Lung Cancer Xenografts via Src/Id1 Pathway
title_short Baicalein Inhibits Orthotopic Human Non-Small Cell Lung Cancer Xenografts via Src/Id1 Pathway
title_sort baicalein inhibits orthotopic human non-small cell lung cancer xenografts via src/id1 pathway
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425425/
https://www.ncbi.nlm.nih.gov/pubmed/30949224
http://dx.doi.org/10.1155/2019/9806062
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