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Genome wide analysis of MADS-box gene family in Brassica oleracea reveals conservation and variation in flower development
BACKGROUND: MADS-box genes play important roles in vegetative growth and reproductive development and are essential for the correct development of plants (particularly inflorescences, flowers, and fruits). However, this gene family has not been identified nor their functions analyzed in Brassica ole...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425688/ https://www.ncbi.nlm.nih.gov/pubmed/30890145 http://dx.doi.org/10.1186/s12870-019-1717-y |
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author | Sheng, Xiao-Guang Zhao, Zhen-Qing Wang, Jian-Sheng Yu, Hui-Fang Shen, Yu-Sen Zeng, Xiao-Yuan Gu, Hong-Hui |
author_facet | Sheng, Xiao-Guang Zhao, Zhen-Qing Wang, Jian-Sheng Yu, Hui-Fang Shen, Yu-Sen Zeng, Xiao-Yuan Gu, Hong-Hui |
author_sort | Sheng, Xiao-Guang |
collection | PubMed |
description | BACKGROUND: MADS-box genes play important roles in vegetative growth and reproductive development and are essential for the correct development of plants (particularly inflorescences, flowers, and fruits). However, this gene family has not been identified nor their functions analyzed in Brassica oleracea. RESULTS: In this study, we performed a whole-genome survey of the complete set of MADS-box genes in B. oleracea. In total, 91 MADS-box transcription factors (TFs) were identified and categorized as type I (Mα, Mβ, Mγ) and type II (MIKC(C), MIKC*) groups according to the phylogeny and gene structure analysis. Among these genes, 59 were randomly distributed on 9 chromosomes, while the other 23 were assigned to 19 scaffolds and 9 genes from NCBI had no location information. Both RNA-sequencing and quantitative real-time-PCR analysis suggested that MIKC genes had more active and complex expression patterns than M type genes and most type II genes showed high flowering-related expression profiles. Additional quantitative real-time-PCR analysis of pedicel and four flower whorls revealed that the structure of the B.oleracea MIKC genes was conserved, but their homologues showed variable expression patterns compared to those in Arabidopsis thaliana. CONCLUSION: This paper gives a detailed overview of the BolMADS genes and their expression patterns. The results obtained in this study provide useful information for understanding the molecular regulation of flower development and further functional characterization of MADS-box genes in B. oleracea. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12870-019-1717-y) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6425688 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-64256882019-04-01 Genome wide analysis of MADS-box gene family in Brassica oleracea reveals conservation and variation in flower development Sheng, Xiao-Guang Zhao, Zhen-Qing Wang, Jian-Sheng Yu, Hui-Fang Shen, Yu-Sen Zeng, Xiao-Yuan Gu, Hong-Hui BMC Plant Biol Research Article BACKGROUND: MADS-box genes play important roles in vegetative growth and reproductive development and are essential for the correct development of plants (particularly inflorescences, flowers, and fruits). However, this gene family has not been identified nor their functions analyzed in Brassica oleracea. RESULTS: In this study, we performed a whole-genome survey of the complete set of MADS-box genes in B. oleracea. In total, 91 MADS-box transcription factors (TFs) were identified and categorized as type I (Mα, Mβ, Mγ) and type II (MIKC(C), MIKC*) groups according to the phylogeny and gene structure analysis. Among these genes, 59 were randomly distributed on 9 chromosomes, while the other 23 were assigned to 19 scaffolds and 9 genes from NCBI had no location information. Both RNA-sequencing and quantitative real-time-PCR analysis suggested that MIKC genes had more active and complex expression patterns than M type genes and most type II genes showed high flowering-related expression profiles. Additional quantitative real-time-PCR analysis of pedicel and four flower whorls revealed that the structure of the B.oleracea MIKC genes was conserved, but their homologues showed variable expression patterns compared to those in Arabidopsis thaliana. CONCLUSION: This paper gives a detailed overview of the BolMADS genes and their expression patterns. The results obtained in this study provide useful information for understanding the molecular regulation of flower development and further functional characterization of MADS-box genes in B. oleracea. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12870-019-1717-y) contains supplementary material, which is available to authorized users. BioMed Central 2019-03-19 /pmc/articles/PMC6425688/ /pubmed/30890145 http://dx.doi.org/10.1186/s12870-019-1717-y Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Sheng, Xiao-Guang Zhao, Zhen-Qing Wang, Jian-Sheng Yu, Hui-Fang Shen, Yu-Sen Zeng, Xiao-Yuan Gu, Hong-Hui Genome wide analysis of MADS-box gene family in Brassica oleracea reveals conservation and variation in flower development |
title | Genome wide analysis of MADS-box gene family in Brassica oleracea reveals conservation and variation in flower development |
title_full | Genome wide analysis of MADS-box gene family in Brassica oleracea reveals conservation and variation in flower development |
title_fullStr | Genome wide analysis of MADS-box gene family in Brassica oleracea reveals conservation and variation in flower development |
title_full_unstemmed | Genome wide analysis of MADS-box gene family in Brassica oleracea reveals conservation and variation in flower development |
title_short | Genome wide analysis of MADS-box gene family in Brassica oleracea reveals conservation and variation in flower development |
title_sort | genome wide analysis of mads-box gene family in brassica oleracea reveals conservation and variation in flower development |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425688/ https://www.ncbi.nlm.nih.gov/pubmed/30890145 http://dx.doi.org/10.1186/s12870-019-1717-y |
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