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Studies of antimicrobial resistance in rare mycobacteria from a nosocomial environment
BACKGROUND: Nontuberculous mycobacteria (NTM) are ubiquitous in nature and recognized agents of opportunistic infection, which is often aggravated by their intrinsic resistance to antimicrobials, poorly defined therapeutic strategies and by the lack of new drugs. However, evaluation of their prevale...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425705/ https://www.ncbi.nlm.nih.gov/pubmed/30890149 http://dx.doi.org/10.1186/s12866-019-1428-4 |
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author | Pereira, Sónia Gonçalves Alarico, Susana Tiago, Igor Reis, Diogo Nunes-Costa, Daniela Cardoso, Olga Maranha, Ana Empadinhas, Nuno |
author_facet | Pereira, Sónia Gonçalves Alarico, Susana Tiago, Igor Reis, Diogo Nunes-Costa, Daniela Cardoso, Olga Maranha, Ana Empadinhas, Nuno |
author_sort | Pereira, Sónia Gonçalves |
collection | PubMed |
description | BACKGROUND: Nontuberculous mycobacteria (NTM) are ubiquitous in nature and recognized agents of opportunistic infection, which is often aggravated by their intrinsic resistance to antimicrobials, poorly defined therapeutic strategies and by the lack of new drugs. However, evaluation of their prevalence in anthropogenic environments and the associated antimicrobial resistance profiles have been neglected. In this work, we sought to determine minimal inhibitory concentrations of 25 antimicrobials against 5 NTM isolates recovered from a tertiary-care hospital surfaces. Antimicrobial susceptibilities of 5 other Corynebacterineae isolated from the same hospital were also determined for their potential clinical relevance. RESULTS: Our phylogenetic study with each of the NTM isolates confirm they belong to Mycobacterium obuense, Mycobacterium mucogenicum and Mycobacterium paragordonae species, the latter initially misidentified as strains of M. gordonae, a species frequently isolated from patients with NTM disease in Portugal. In contrast to other strains, the M. obuense and M. mucogenicum examined here were resistant to several of the CLSI-recommended drugs, suggestive of multidrug-resistant profiles. Surprisingly, M. obuense was susceptible to vancomycin. Their genomes were sequenced allowing detection of gene erm (erythromycin resistance methylase) in M. obuense, explaining its resistance to clarithromycin. Remarkably, and unlike other strains of the genus, the Corynebacterium isolates were highly resistant to penicillin, ciprofloxacin and linezolid. CONCLUSIONS: This study highlights the importance of implementing effective measures to screen, accurately identify and control viable NTM and closely related bacteria in hospital settings. Our report on the occurrence of rare NTM species with antibiotic susceptibility profiles that are distinct from those of the corresponding Type strains, along with unexpected resistance mechanisms detected seem to suggest that resistance may be more common than previously thought and also a potential threat to frail and otherwise vulnerable inpatients. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12866-019-1428-4) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6425705 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-64257052019-04-01 Studies of antimicrobial resistance in rare mycobacteria from a nosocomial environment Pereira, Sónia Gonçalves Alarico, Susana Tiago, Igor Reis, Diogo Nunes-Costa, Daniela Cardoso, Olga Maranha, Ana Empadinhas, Nuno BMC Microbiol Research Article BACKGROUND: Nontuberculous mycobacteria (NTM) are ubiquitous in nature and recognized agents of opportunistic infection, which is often aggravated by their intrinsic resistance to antimicrobials, poorly defined therapeutic strategies and by the lack of new drugs. However, evaluation of their prevalence in anthropogenic environments and the associated antimicrobial resistance profiles have been neglected. In this work, we sought to determine minimal inhibitory concentrations of 25 antimicrobials against 5 NTM isolates recovered from a tertiary-care hospital surfaces. Antimicrobial susceptibilities of 5 other Corynebacterineae isolated from the same hospital were also determined for their potential clinical relevance. RESULTS: Our phylogenetic study with each of the NTM isolates confirm they belong to Mycobacterium obuense, Mycobacterium mucogenicum and Mycobacterium paragordonae species, the latter initially misidentified as strains of M. gordonae, a species frequently isolated from patients with NTM disease in Portugal. In contrast to other strains, the M. obuense and M. mucogenicum examined here were resistant to several of the CLSI-recommended drugs, suggestive of multidrug-resistant profiles. Surprisingly, M. obuense was susceptible to vancomycin. Their genomes were sequenced allowing detection of gene erm (erythromycin resistance methylase) in M. obuense, explaining its resistance to clarithromycin. Remarkably, and unlike other strains of the genus, the Corynebacterium isolates were highly resistant to penicillin, ciprofloxacin and linezolid. CONCLUSIONS: This study highlights the importance of implementing effective measures to screen, accurately identify and control viable NTM and closely related bacteria in hospital settings. Our report on the occurrence of rare NTM species with antibiotic susceptibility profiles that are distinct from those of the corresponding Type strains, along with unexpected resistance mechanisms detected seem to suggest that resistance may be more common than previously thought and also a potential threat to frail and otherwise vulnerable inpatients. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12866-019-1428-4) contains supplementary material, which is available to authorized users. BioMed Central 2019-03-19 /pmc/articles/PMC6425705/ /pubmed/30890149 http://dx.doi.org/10.1186/s12866-019-1428-4 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Pereira, Sónia Gonçalves Alarico, Susana Tiago, Igor Reis, Diogo Nunes-Costa, Daniela Cardoso, Olga Maranha, Ana Empadinhas, Nuno Studies of antimicrobial resistance in rare mycobacteria from a nosocomial environment |
title | Studies of antimicrobial resistance in rare mycobacteria from a nosocomial environment |
title_full | Studies of antimicrobial resistance in rare mycobacteria from a nosocomial environment |
title_fullStr | Studies of antimicrobial resistance in rare mycobacteria from a nosocomial environment |
title_full_unstemmed | Studies of antimicrobial resistance in rare mycobacteria from a nosocomial environment |
title_short | Studies of antimicrobial resistance in rare mycobacteria from a nosocomial environment |
title_sort | studies of antimicrobial resistance in rare mycobacteria from a nosocomial environment |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425705/ https://www.ncbi.nlm.nih.gov/pubmed/30890149 http://dx.doi.org/10.1186/s12866-019-1428-4 |
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