Cargando…

Cytology Microarray on Cell Block Preparation: A Novel Diagnostic Approach in Fluid Cytology

BACKGROUND: The cytological examination of serous body effusions to diagnose and stage malignancy is well accepted in clinical medicine. Conventional smear (CS) and cell block (CB) study has to be complemented with immunohistochemistry (IHC) for a definitive diagnosis of malignancy and also to diffe...

Descripción completa

Detalles Bibliográficos
Autores principales: Bandyopadhyay, Arghya, Bhattacharyya, Soumi, Roy, Shreosee, Majumdar, Kaushik, Bose, Kaushik, Boler, Anup K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425782/
https://www.ncbi.nlm.nih.gov/pubmed/30992641
http://dx.doi.org/10.4103/JOC.JOC_15_17
_version_ 1783404902002720768
author Bandyopadhyay, Arghya
Bhattacharyya, Soumi
Roy, Shreosee
Majumdar, Kaushik
Bose, Kaushik
Boler, Anup K.
author_facet Bandyopadhyay, Arghya
Bhattacharyya, Soumi
Roy, Shreosee
Majumdar, Kaushik
Bose, Kaushik
Boler, Anup K.
author_sort Bandyopadhyay, Arghya
collection PubMed
description BACKGROUND: The cytological examination of serous body effusions to diagnose and stage malignancy is well accepted in clinical medicine. Conventional smear (CS) and cell block (CB) study has to be complemented with immunohistochemistry (IHC) for a definitive diagnosis of malignancy and also to differentiate it from reactive mesothelial cells. Cytology microarray (CMA) is a modification of tissue microarray which involves core needle biopsy of multiple cell blocks and embedding it in a single block. AIM: The aim of this study was to assess the effectiveness of IHC technique in CMA for rapid diagnosis of malignancy and to reduce the cost of testing. MATERIALS AND METHODS: In this study, 82 pleural fluids were collected and subjected to CS and CB study followed by IHC in CMA blocks. Six commonly used antibodies were applied to confirm malignancy and diagnose the primary. RESULTS: Nineteen cases were diagnosed as malignancy by CB method. MOC-31 confirmed adenocarcinoma deposit in 67% cases of which 44% were proved to be of lung primary by TTF1. CONCLUSIONS: IHC on CMA blocks of effusion fluids is a very effective technique that can significantly reduce the cost of testing by >70%.
format Online
Article
Text
id pubmed-6425782
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Medknow Publications & Media Pvt Ltd
record_format MEDLINE/PubMed
spelling pubmed-64257822019-04-17 Cytology Microarray on Cell Block Preparation: A Novel Diagnostic Approach in Fluid Cytology Bandyopadhyay, Arghya Bhattacharyya, Soumi Roy, Shreosee Majumdar, Kaushik Bose, Kaushik Boler, Anup K. J Cytol Original Article BACKGROUND: The cytological examination of serous body effusions to diagnose and stage malignancy is well accepted in clinical medicine. Conventional smear (CS) and cell block (CB) study has to be complemented with immunohistochemistry (IHC) for a definitive diagnosis of malignancy and also to differentiate it from reactive mesothelial cells. Cytology microarray (CMA) is a modification of tissue microarray which involves core needle biopsy of multiple cell blocks and embedding it in a single block. AIM: The aim of this study was to assess the effectiveness of IHC technique in CMA for rapid diagnosis of malignancy and to reduce the cost of testing. MATERIALS AND METHODS: In this study, 82 pleural fluids were collected and subjected to CS and CB study followed by IHC in CMA blocks. Six commonly used antibodies were applied to confirm malignancy and diagnose the primary. RESULTS: Nineteen cases were diagnosed as malignancy by CB method. MOC-31 confirmed adenocarcinoma deposit in 67% cases of which 44% were proved to be of lung primary by TTF1. CONCLUSIONS: IHC on CMA blocks of effusion fluids is a very effective technique that can significantly reduce the cost of testing by >70%. Medknow Publications & Media Pvt Ltd 2019 /pmc/articles/PMC6425782/ /pubmed/30992641 http://dx.doi.org/10.4103/JOC.JOC_15_17 Text en Copyright: © 2019 Journal of Cytology http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Bandyopadhyay, Arghya
Bhattacharyya, Soumi
Roy, Shreosee
Majumdar, Kaushik
Bose, Kaushik
Boler, Anup K.
Cytology Microarray on Cell Block Preparation: A Novel Diagnostic Approach in Fluid Cytology
title Cytology Microarray on Cell Block Preparation: A Novel Diagnostic Approach in Fluid Cytology
title_full Cytology Microarray on Cell Block Preparation: A Novel Diagnostic Approach in Fluid Cytology
title_fullStr Cytology Microarray on Cell Block Preparation: A Novel Diagnostic Approach in Fluid Cytology
title_full_unstemmed Cytology Microarray on Cell Block Preparation: A Novel Diagnostic Approach in Fluid Cytology
title_short Cytology Microarray on Cell Block Preparation: A Novel Diagnostic Approach in Fluid Cytology
title_sort cytology microarray on cell block preparation: a novel diagnostic approach in fluid cytology
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425782/
https://www.ncbi.nlm.nih.gov/pubmed/30992641
http://dx.doi.org/10.4103/JOC.JOC_15_17
work_keys_str_mv AT bandyopadhyayarghya cytologymicroarrayoncellblockpreparationanoveldiagnosticapproachinfluidcytology
AT bhattacharyyasoumi cytologymicroarrayoncellblockpreparationanoveldiagnosticapproachinfluidcytology
AT royshreosee cytologymicroarrayoncellblockpreparationanoveldiagnosticapproachinfluidcytology
AT majumdarkaushik cytologymicroarrayoncellblockpreparationanoveldiagnosticapproachinfluidcytology
AT bosekaushik cytologymicroarrayoncellblockpreparationanoveldiagnosticapproachinfluidcytology
AT boleranupk cytologymicroarrayoncellblockpreparationanoveldiagnosticapproachinfluidcytology