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In situ visualization of ozone in the brains of mice with depression phenotypes by using a new near-infrared fluorescence probe
Ozone (O(3)), one of the reactive oxygen species (ROS), is deeply involved in diseases including depression. However, the lack of appropriate in situ detection methods suitable for the complex biological context of brain impedes uncovering the exact relationship between depression and changes in the...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Royal Society of Chemistry
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425856/ https://www.ncbi.nlm.nih.gov/pubmed/30997001 http://dx.doi.org/10.1039/c8sc04891f |
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author | Li, Ping Wang, Jijuan Wang, Xin Ding, Qi Bai, Xiaoyi Zhang, Yandi Su, Di Zhang, Wei Zhang, Wen Tang, Bo |
author_facet | Li, Ping Wang, Jijuan Wang, Xin Ding, Qi Bai, Xiaoyi Zhang, Yandi Su, Di Zhang, Wei Zhang, Wen Tang, Bo |
author_sort | Li, Ping |
collection | PubMed |
description | Ozone (O(3)), one of the reactive oxygen species (ROS), is deeply involved in diseases including depression. However, the lack of appropriate in situ detection methods suitable for the complex biological context of brain impedes uncovering the exact relationship between depression and changes in the O(3) level. Therefore, we developed a near-infrared (NIR) fluorescent probe (ACy7) for the direct visualization of O(3) in mice brains. The specific cycloaddition reaction between O(3) and the terminal double bond of the butenyl group extends the conjugation of the “pre-” heptamethine cyanine system, which emits NIR fluorescence of heptamethine cyanine. This makes the ACy7 specific, highly sensitive and able to deeply penetrate tissue. Using ACy7, we found that under glutamate stimulation, the O(3) content in PC12 cells was significantly higher than that in control cells. By imaging analysis on the brains of mice, we revealed for the first time that the levels of O(3) in mice with depression phenotypes were markedly higher than that in control mice. Intriguingly, experimental results unravelled that excess O(3) promoted high expression of the pro-inflammatory cytokine interleukin-8 (IL-8), which ultimately induced depression phenotypes. Our work demonstrates the pivotal role of elevated O(3) in depression and provides a fresh entry point for exploring oxidative stress contributing to depression. |
format | Online Article Text |
id | pubmed-6425856 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-64258562019-04-17 In situ visualization of ozone in the brains of mice with depression phenotypes by using a new near-infrared fluorescence probe Li, Ping Wang, Jijuan Wang, Xin Ding, Qi Bai, Xiaoyi Zhang, Yandi Su, Di Zhang, Wei Zhang, Wen Tang, Bo Chem Sci Chemistry Ozone (O(3)), one of the reactive oxygen species (ROS), is deeply involved in diseases including depression. However, the lack of appropriate in situ detection methods suitable for the complex biological context of brain impedes uncovering the exact relationship between depression and changes in the O(3) level. Therefore, we developed a near-infrared (NIR) fluorescent probe (ACy7) for the direct visualization of O(3) in mice brains. The specific cycloaddition reaction between O(3) and the terminal double bond of the butenyl group extends the conjugation of the “pre-” heptamethine cyanine system, which emits NIR fluorescence of heptamethine cyanine. This makes the ACy7 specific, highly sensitive and able to deeply penetrate tissue. Using ACy7, we found that under glutamate stimulation, the O(3) content in PC12 cells was significantly higher than that in control cells. By imaging analysis on the brains of mice, we revealed for the first time that the levels of O(3) in mice with depression phenotypes were markedly higher than that in control mice. Intriguingly, experimental results unravelled that excess O(3) promoted high expression of the pro-inflammatory cytokine interleukin-8 (IL-8), which ultimately induced depression phenotypes. Our work demonstrates the pivotal role of elevated O(3) in depression and provides a fresh entry point for exploring oxidative stress contributing to depression. Royal Society of Chemistry 2019-01-17 /pmc/articles/PMC6425856/ /pubmed/30997001 http://dx.doi.org/10.1039/c8sc04891f Text en This journal is © The Royal Society of Chemistry 2019 https://creativecommons.org/licenses/by-nc/3.0/This article is freely available. This article is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported Licence (CC BY-NC 3.0) |
spellingShingle | Chemistry Li, Ping Wang, Jijuan Wang, Xin Ding, Qi Bai, Xiaoyi Zhang, Yandi Su, Di Zhang, Wei Zhang, Wen Tang, Bo In situ visualization of ozone in the brains of mice with depression phenotypes by using a new near-infrared fluorescence probe |
title |
In situ visualization of ozone in the brains of mice with depression phenotypes by using a new near-infrared fluorescence probe
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title_full |
In situ visualization of ozone in the brains of mice with depression phenotypes by using a new near-infrared fluorescence probe
|
title_fullStr |
In situ visualization of ozone in the brains of mice with depression phenotypes by using a new near-infrared fluorescence probe
|
title_full_unstemmed |
In situ visualization of ozone in the brains of mice with depression phenotypes by using a new near-infrared fluorescence probe
|
title_short |
In situ visualization of ozone in the brains of mice with depression phenotypes by using a new near-infrared fluorescence probe
|
title_sort | in situ visualization of ozone in the brains of mice with depression phenotypes by using a new near-infrared fluorescence probe |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425856/ https://www.ncbi.nlm.nih.gov/pubmed/30997001 http://dx.doi.org/10.1039/c8sc04891f |
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