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Agrobacterium rhizogenes-mediated hairy roots transformation as a tool for exploring aluminum-responsive genes function

AIM: To develop a useful alternative approach to evaluate the gene function in hairy roots. METHODS: Arabidopsis and tobacco (wild-type or mutant) were a host for Agrobacterium rhizogenes transformation. RESULTS: The hairy roots formation efficiency ranged from 53 to 98% in tobacco and 53 to 66% in...

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Detalles Bibliográficos
Autores principales: Daspute, Abhijit A, Yunxuan, Xian, Gu, Minghua, Kobayashi, Yuriko, Wagh, Sopan, Panche, Archana, Koyama, Hiroyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Future Science Ltd 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6426172/
https://www.ncbi.nlm.nih.gov/pubmed/30906565
http://dx.doi.org/10.4155/fsoa-2018-0065
Descripción
Sumario:AIM: To develop a useful alternative approach to evaluate the gene function in hairy roots. METHODS: Arabidopsis and tobacco (wild-type or mutant) were a host for Agrobacterium rhizogenes transformation. RESULTS: The hairy roots formation efficiency ranged from 53 to 98% in tobacco and 53 to 66% in Arabidopsis. Hairy and intact roots showed similar gene expression pattern in response to salt and aluminum stress. Genomic polymerase chain reaction and fluorescent images showed high rate (>80%) of co-integration of T-DNAs and uniform cell transformation without use of any antibiotic selection. Whole processes of hairy roots were completed within 1 month after the infection of Agrobacterium. CONCLUSION: Aluminum-responsive orthologous gene function could be evaluated by NtSTOP1-KD and Atstop1 as a host for hairy roots transformation.