Cargando…

Agrobacterium rhizogenes-mediated hairy roots transformation as a tool for exploring aluminum-responsive genes function

AIM: To develop a useful alternative approach to evaluate the gene function in hairy roots. METHODS: Arabidopsis and tobacco (wild-type or mutant) were a host for Agrobacterium rhizogenes transformation. RESULTS: The hairy roots formation efficiency ranged from 53 to 98% in tobacco and 53 to 66% in...

Descripción completa

Detalles Bibliográficos
Autores principales: Daspute, Abhijit A, Yunxuan, Xian, Gu, Minghua, Kobayashi, Yuriko, Wagh, Sopan, Panche, Archana, Koyama, Hiroyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Future Science Ltd 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6426172/
https://www.ncbi.nlm.nih.gov/pubmed/30906565
http://dx.doi.org/10.4155/fsoa-2018-0065
_version_ 1783404960081248256
author Daspute, Abhijit A
Yunxuan, Xian
Gu, Minghua
Kobayashi, Yuriko
Wagh, Sopan
Panche, Archana
Koyama, Hiroyuki
author_facet Daspute, Abhijit A
Yunxuan, Xian
Gu, Minghua
Kobayashi, Yuriko
Wagh, Sopan
Panche, Archana
Koyama, Hiroyuki
author_sort Daspute, Abhijit A
collection PubMed
description AIM: To develop a useful alternative approach to evaluate the gene function in hairy roots. METHODS: Arabidopsis and tobacco (wild-type or mutant) were a host for Agrobacterium rhizogenes transformation. RESULTS: The hairy roots formation efficiency ranged from 53 to 98% in tobacco and 53 to 66% in Arabidopsis. Hairy and intact roots showed similar gene expression pattern in response to salt and aluminum stress. Genomic polymerase chain reaction and fluorescent images showed high rate (>80%) of co-integration of T-DNAs and uniform cell transformation without use of any antibiotic selection. Whole processes of hairy roots were completed within 1 month after the infection of Agrobacterium. CONCLUSION: Aluminum-responsive orthologous gene function could be evaluated by NtSTOP1-KD and Atstop1 as a host for hairy roots transformation.
format Online
Article
Text
id pubmed-6426172
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Future Science Ltd
record_format MEDLINE/PubMed
spelling pubmed-64261722019-03-22 Agrobacterium rhizogenes-mediated hairy roots transformation as a tool for exploring aluminum-responsive genes function Daspute, Abhijit A Yunxuan, Xian Gu, Minghua Kobayashi, Yuriko Wagh, Sopan Panche, Archana Koyama, Hiroyuki Future Sci OA Research Article AIM: To develop a useful alternative approach to evaluate the gene function in hairy roots. METHODS: Arabidopsis and tobacco (wild-type or mutant) were a host for Agrobacterium rhizogenes transformation. RESULTS: The hairy roots formation efficiency ranged from 53 to 98% in tobacco and 53 to 66% in Arabidopsis. Hairy and intact roots showed similar gene expression pattern in response to salt and aluminum stress. Genomic polymerase chain reaction and fluorescent images showed high rate (>80%) of co-integration of T-DNAs and uniform cell transformation without use of any antibiotic selection. Whole processes of hairy roots were completed within 1 month after the infection of Agrobacterium. CONCLUSION: Aluminum-responsive orthologous gene function could be evaluated by NtSTOP1-KD and Atstop1 as a host for hairy roots transformation. Future Science Ltd 2019-02-08 /pmc/articles/PMC6426172/ /pubmed/30906565 http://dx.doi.org/10.4155/fsoa-2018-0065 Text en © 2019 Hiroyuki Koyama This work is licensed under a Creative Commons Attribution 4.0 License (http://creativecommons.org/licenses/by/4.0/)
spellingShingle Research Article
Daspute, Abhijit A
Yunxuan, Xian
Gu, Minghua
Kobayashi, Yuriko
Wagh, Sopan
Panche, Archana
Koyama, Hiroyuki
Agrobacterium rhizogenes-mediated hairy roots transformation as a tool for exploring aluminum-responsive genes function
title Agrobacterium rhizogenes-mediated hairy roots transformation as a tool for exploring aluminum-responsive genes function
title_full Agrobacterium rhizogenes-mediated hairy roots transformation as a tool for exploring aluminum-responsive genes function
title_fullStr Agrobacterium rhizogenes-mediated hairy roots transformation as a tool for exploring aluminum-responsive genes function
title_full_unstemmed Agrobacterium rhizogenes-mediated hairy roots transformation as a tool for exploring aluminum-responsive genes function
title_short Agrobacterium rhizogenes-mediated hairy roots transformation as a tool for exploring aluminum-responsive genes function
title_sort agrobacterium rhizogenes-mediated hairy roots transformation as a tool for exploring aluminum-responsive genes function
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6426172/
https://www.ncbi.nlm.nih.gov/pubmed/30906565
http://dx.doi.org/10.4155/fsoa-2018-0065
work_keys_str_mv AT dasputeabhijita agrobacteriumrhizogenesmediatedhairyrootstransformationasatoolforexploringaluminumresponsivegenesfunction
AT yunxuanxian agrobacteriumrhizogenesmediatedhairyrootstransformationasatoolforexploringaluminumresponsivegenesfunction
AT guminghua agrobacteriumrhizogenesmediatedhairyrootstransformationasatoolforexploringaluminumresponsivegenesfunction
AT kobayashiyuriko agrobacteriumrhizogenesmediatedhairyrootstransformationasatoolforexploringaluminumresponsivegenesfunction
AT waghsopan agrobacteriumrhizogenesmediatedhairyrootstransformationasatoolforexploringaluminumresponsivegenesfunction
AT panchearchana agrobacteriumrhizogenesmediatedhairyrootstransformationasatoolforexploringaluminumresponsivegenesfunction
AT koyamahiroyuki agrobacteriumrhizogenesmediatedhairyrootstransformationasatoolforexploringaluminumresponsivegenesfunction