Bcd1p controls RNA loading of the core protein Nop58 during C/D box snoRNP biogenesis

Biogenesis of eukaryotic box C/D small nucleolar ribonucleoproteins (C/D snoRNPs) is guided by conserved trans-acting factors that act collectively to assemble the core proteins SNU13/Snu13, NOP58/Nop58, NOP56/Nop56, FBL/Nop1, and box C/D small nucleolar RNAs (C/D snoRNAs), in human and in yeast, re...

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Autores principales: Paul, Arnaud, Tiotiu, Decebal, Bragantini, Benoît, Marty, Hélène, Charpentier, Bruno, Massenet, Séverine, Labialle, Stéphane
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6426285/
https://www.ncbi.nlm.nih.gov/pubmed/30700579
http://dx.doi.org/10.1261/rna.067967.118
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author Paul, Arnaud
Tiotiu, Decebal
Bragantini, Benoît
Marty, Hélène
Charpentier, Bruno
Massenet, Séverine
Labialle, Stéphane
author_facet Paul, Arnaud
Tiotiu, Decebal
Bragantini, Benoît
Marty, Hélène
Charpentier, Bruno
Massenet, Séverine
Labialle, Stéphane
author_sort Paul, Arnaud
collection PubMed
description Biogenesis of eukaryotic box C/D small nucleolar ribonucleoproteins (C/D snoRNPs) is guided by conserved trans-acting factors that act collectively to assemble the core proteins SNU13/Snu13, NOP58/Nop58, NOP56/Nop56, FBL/Nop1, and box C/D small nucleolar RNAs (C/D snoRNAs), in human and in yeast, respectively. This finely elaborated process involves the sequential interplay of snoRNP-related proteins and RNA through the formation of transient pre-RNP complexes. BCD1/Bcd1 protein is essential for yeast cell growth and for the specific accumulation of box C/D snoRNAs. In this work, chromatin, RNA, and protein immunoprecipitation assays revealed the ordered loading of several snoRNP-related proteins on immature and mature snoRNA species. Our results identify Bcd1p as an assembly factor of C/D snoRNP biogenesis that is likely recruited cotranscriptionally and that directs the loading of the core protein Nop58 on RNA.
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spelling pubmed-64262852020-04-01 Bcd1p controls RNA loading of the core protein Nop58 during C/D box snoRNP biogenesis Paul, Arnaud Tiotiu, Decebal Bragantini, Benoît Marty, Hélène Charpentier, Bruno Massenet, Séverine Labialle, Stéphane RNA Article Biogenesis of eukaryotic box C/D small nucleolar ribonucleoproteins (C/D snoRNPs) is guided by conserved trans-acting factors that act collectively to assemble the core proteins SNU13/Snu13, NOP58/Nop58, NOP56/Nop56, FBL/Nop1, and box C/D small nucleolar RNAs (C/D snoRNAs), in human and in yeast, respectively. This finely elaborated process involves the sequential interplay of snoRNP-related proteins and RNA through the formation of transient pre-RNP complexes. BCD1/Bcd1 protein is essential for yeast cell growth and for the specific accumulation of box C/D snoRNAs. In this work, chromatin, RNA, and protein immunoprecipitation assays revealed the ordered loading of several snoRNP-related proteins on immature and mature snoRNA species. Our results identify Bcd1p as an assembly factor of C/D snoRNP biogenesis that is likely recruited cotranscriptionally and that directs the loading of the core protein Nop58 on RNA. Cold Spring Harbor Laboratory Press 2019-04 /pmc/articles/PMC6426285/ /pubmed/30700579 http://dx.doi.org/10.1261/rna.067967.118 Text en © 2019 Paul et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Article
Paul, Arnaud
Tiotiu, Decebal
Bragantini, Benoît
Marty, Hélène
Charpentier, Bruno
Massenet, Séverine
Labialle, Stéphane
Bcd1p controls RNA loading of the core protein Nop58 during C/D box snoRNP biogenesis
title Bcd1p controls RNA loading of the core protein Nop58 during C/D box snoRNP biogenesis
title_full Bcd1p controls RNA loading of the core protein Nop58 during C/D box snoRNP biogenesis
title_fullStr Bcd1p controls RNA loading of the core protein Nop58 during C/D box snoRNP biogenesis
title_full_unstemmed Bcd1p controls RNA loading of the core protein Nop58 during C/D box snoRNP biogenesis
title_short Bcd1p controls RNA loading of the core protein Nop58 during C/D box snoRNP biogenesis
title_sort bcd1p controls rna loading of the core protein nop58 during c/d box snornp biogenesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6426285/
https://www.ncbi.nlm.nih.gov/pubmed/30700579
http://dx.doi.org/10.1261/rna.067967.118
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